Transplanting and domestication method of strawberry pollen detoxified tissue culture seedlings

Abstract

The invention discloses a transplanting and acclimatizating method of virus-free tissue culture seedlings of strawberry pollen. The method comprises the following steps: (1) collecting, washing and sterilizing pollen; (2) inducing differentiation of pollen into seedlings; (3) rapidly propagating seedlings and rooting; (4) emerging seedlings and washing the seedlings; (5) transplanting; and (6) acclimatizating. The method is capable of transplanting by directly washing a culture medium without the need of hardening the seedlings; a plastic arched shed is built on a seedling bed; subsequently, the plastic arched shed is sealed for three days; then the temperature, the substrate humidity, the illumination time and the illumination intensity in an arched film are effectively controlled. Through the method, the survival rate of the virus-free tissue culture seedlings of the pollen can reach 100%; the probability of obtaining virus-free seedlings is greatly improved; meanwhile, the virus removal rate of the strawberry pollen can reach 100%; the method is free of virus detection process; the period from virus removal to large-scale production of the seedlings is shortened; the production cost and the labor cost are effectively reduced; the production benefit is greatly improved; large batches of high-quality virus-free seedlings are provided; the technical support is supplied to the sustainable and healthy industrialization development of strawberry.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture seedlings, in particular to a method for transplanting and domesticating strawberry pollen detoxification tissue culture seedlings. Background technique [0002] The test-tube seedlings of pollen plants mainly rely on the sugar in the medium for heterotrophic metabolism in the medium, and the humidity and temperature in the test-tube are relatively stable. Once moved into the soil, the physiological and ecological environment will undergo great changes. Rapid adaptation to this change will cause a large number of deaths, especially when the natural temperature changes frequently at the right time for transplanting, and it is difficult to survive on schedule. At present, it is generally adopted that the bottle seedlings have two stages of seedling hardening process: one is to move the tissue culture bottle seedlings to the outside and place them under natural light for 2 d to 3 d to tr...

AI Technical Summary

Problems solved by technology

[0002] The test-tube seedlings of pollen plants mainly rely on the sugar in the medium for heterotrophic metabolism on the medium, and the humidity and temperature in the test tube are relatively stable. Quickly adapt to this change and cause a large number of deaths, especially when transplanting is suitable for frequent changes in natural temperature, it is difficult to survive when transplanting on schedule

At present, it is generally adopted that bottled seedlings have two stages of seedling hardening process: one is to move the bottled seedlings outdoors and place them under natural light for 2-3 days to practice seedlings; It takes a short time to change from heterotrophic to autotrophic environment, and the seedlings are not suitable yet. Open the bottle cap again, and the pollution rate will rise rapidly to 30% to 60%, which will greatly reduce the resistance of the seedlings, and the survival rate will drop significantly after transplanting.