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Method for tissue culture of Canadian cercis chinensis

A technology of Canadian bauhinia and tissue culture, applied in the field of plant tissue culture, can solve the problem that there is no Canadian bauhinia tissue culture method, etc.

Inactive Publication Date: 2016-10-12
WUHU OUBIAO AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is no report on the tissue culture method of Bauhinia canadensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Selection of explants: Take the young stem tips of Bauhinia canadensis with a diameter of 2-5mm or the stem segments with at least one single bud as explants, cut off the leaves and cut the stem tips or at least one single bud Cut the stem section into 2-3cm long for later use;

[0028] 2) Disinfection of explants: put the above-mentioned stem section into a 20KHz ultrasonic cleaning filled with tap water and keep the temperature at 30°C for 30 minutes, then rinse with tap water for 10 minutes; then disinfect with 75% alcohol on the ultra-clean workbench for 25 seconds, Treat in 0.1% mercuric chloride solution for 7 minutes, then rinse 4 times with sterile water, and place in a sterile petri dish for later use;

[0029] 3) Initial induction culture: Cut off 2-3 mm of each explant after sterilization, and insert the base of the explant downward into the initial induction medium for culture. The culture conditions are light intensity 1500-2000Lx, photoperiod 10-14h / d ...

Embodiment 2

[0035] 1) Selection of explants: 1) Selection of explants: take the young shoot tips of Bauhinia canadensis with a diameter of 2-5mm in the current year or the stem section with at least one single bud as explants, cut off the leaves and remove the stems Cut the pointed or at least one single-bud stem section into 2-3cm long for later use;

[0036]2) Disinfection of explants: Put the above-mentioned stem section into a 20KHz ultrasonic cleaner filled with tap water and keep the temperature at 35°C for 45 minutes, then rinse with tap water for 20 minutes; then disinfect with 75% alcohol on an ultra-clean workbench for 45 seconds , treated in 0.1% mercuric chloride solution for 12 minutes, then washed 5 times with sterile water, and placed in a sterile petri dish for later use;

[0037] 3) Initial induction culture: Cut the sterilized explants by 2-3 mm each, and insert the base of the explants downward into the initial induction medium for culture. The culture conditions are li...

Embodiment 3

[0043] 1) Selection of explants: Take the young stem tips of Bauhinia canadensis with a diameter of 2-5mm or the stem segments with at least one single bud as explants, cut off the leaves and cut the stem tips or at least one single bud Cut the stems into 2-3cm long for later use;

[0044] 2) Disinfection of explants: Put the above-mentioned stem section into a 20KHz ultrasonic cleaner filled with tap water and keep the temperature at 35°C for 35 minutes, then rinse with tap water for 15 minutes; then disinfect with 75% alcohol on an ultra-clean workbench for 35 seconds , treated in 0.1% mercuric chloride solution for 10 minutes, then washed 5 times with sterile water, and placed in a sterile petri dish for later use;

[0045] 3) Initial induction culture: Cut the sterilized explants by 2-3 mm each, and insert the base of the explants downward into the initial induction medium for culture. The culture conditions are light intensity 1500-2000Lx, photoperiod 10-14h / d, the temp...

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PUM

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Abstract

The invention relates to a method for tissue culture of Canadian cercis chinensis. The method comprises a step 1) of explant selection, a step 2) of explant disinfection, a step 3) of initial induced culture, a step 4) of multiplication culture, a step 5) of rooting culture and a step 6) of seedling exercising and transplantation. By the adoption of the method for breeding the Canadian cercis chinensis, the production cost can be reduced, and the breeding speed and survival rate are improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the technical field of a Canadian bauhinia tissue culture method. Background technique [0002] Canadian redbud (Cercis canadensis) is a small tree of the genus Bauhinia in the family Fabaceae. It is native to the United States and is distributed in the eastern and midwestern United States and Ontario, Canada. The wood is dense, hard, with fine stripes, the height of the tree is 7-11m, the crown width is 7.5-10.5m, the main trunk is short, and there are several main branches; the flowering period is long, blooming from April to May, flowers first and then leaves, flowers with roses Pink, lavender, rarely white; leaves waxy; pods mature in July-August. It is 5cm long, 1.4cm wide in the middle, and the two ends are acuminate. There are 4-5 seeds in the pod. Canadian Bauhinia is hardy to cold. It likes plenty of sunshine, but is slightly shade-tolerant. It blooms we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 戴勤王冬梅李慧珍
Owner WUHU OUBIAO AGRI DEV
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