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Chinese angelica acidic polysaccharide component, and preparation method and application thereof

A technology of acidic polysaccharides and extraction methods, applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as activity differences, and achieve the goal of improving tolerance, significant anti-radiation effect, and enhancing immunity The effect of action

Inactive Publication Date: 2016-10-05
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] According to the above, it can be seen that there are many polysaccharide components of Angelica sinensis isolated at present, but the activities of different components are very different due to the different polysaccharide structures and properties.

Method used

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  • Chinese angelica acidic polysaccharide component, and preparation method and application thereof
  • Chinese angelica acidic polysaccharide component, and preparation method and application thereof
  • Chinese angelica acidic polysaccharide component, and preparation method and application thereof

Examples

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Embodiment 1

[0051] Weigh 1 kg of angelica root, crush it into a coarse powder; reflux extract with 4000mL of absolute ethanol for 2 hours (skim off), filter out the ethanol extract, and reprocess the root residue of angelica root (hereinafter also referred to as medicinal residue) according to the above method once , filter out the ethanol extract, evaporate the residue to dry ethanol, dry at 40°C, and extract with 6000 mL of 1.0 mol / L NaOH reflux three times, each time for 2 hours. The combined water extracts were concentrated to 1000mL under reduced pressure with a rotary evaporator at 60°C, pre-cooled at 4°C for 4 hours, then added 2 times the volume of absolute ethanol, left standing at 4°C for 12 hours, centrifuged at 4000g for 15 minutes, and the precipitate was collected. The precipitate was dissolved in 400 mL of water, freeze-thawed 5 times, centrifuged, and the precipitate was discarded; the Sevag method (i.e. V 多糖溶液 :V 氯仿 :V 正丁醇 =20︰4︰1 Mix and shake for half an hour, centrif...

Embodiment 2

[0053] Weigh 1 kg of angelica root, crush it into a coarse powder; reflux extract with 8000mL 95% ethanol for 2 hours, filter out the ethanol extract, evaporate the dregs to dry with ethanol, dry at 50°C, then reflux extract twice with 8000mL 1.2mol / L NaOH , 3 hours each time. The water extracts were combined and concentrated to 800mL under reduced pressure with a rotary evaporator at 60°C. After pre-cooling at 4°C for 6 hours, 2 times the volume of absolute ethanol was added. After standing at 4°C for 12 hours, centrifuge at 3000g for 15 minutes to collect the precipitate. The precipitate was dissolved in 300 mL of water, freeze-thawed 10 times, centrifuged, and the precipitate was discarded. The aqueous solution was dialyzed against distilled water for 48 hours to remove small molecule impurities using a dialysis membrane with a molecular weight cut-off of 8000 Da, and the solution in the dialysis bag was collected and concentrated to 120 mL under reduced pressure by a rotar...

Embodiment 3

[0055]Weigh 1kg of angelica root and crush it into a coarse powder; wet it with 2000mL 95% ethanol; percolate with 8000ml 95% ethanol, evaporate the dregs to dry the ethanol, dry at 50°C, and reflux extract twice with 8000mL 1.0mol / L KOH , 3 hours each time. The water extracts were combined and concentrated to 800mL under reduced pressure with a rotary evaporator at 60°C. After pre-cooling at 4°C for 6 hours, 3 times the volume of absolute ethanol was added. After standing at 4°C for 12 hours, centrifuge at 3000g for 10 minutes to collect the precipitate. The precipitate was dissolved in 300mL of water, repeated freezing and thawing 5 times, centrifuged, and the precipitate was discarded. Add calcium hydroxide alkali solution to adjust the pH to 11, place it overnight at 4°C, filter it with suction, adjust the filtrate to pH 5 with sulfuric acid solution, place it at 4°C overnight, and then filter it with suction, add KOH alkali solution to the filtrate to adjust pH 7, and use...

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Abstract

The invention relates to a Chinese angelica acidic polysaccharide component, a preparation method thereof, and an application thereof in the preparation of drugs and health products for preventing cancers, enhancing immunity and preventing radiation damages, and belongs to the field of traditional Chinese medicine modernization. The method comprises the following steps: degreasing Chinese angelica roots, carrying out refluxing extraction with alkaline water, precipitating polysaccharides with alcohol, removing proteins, carrying out dialysis, carrying out anion exchange column DEAE serial column grading, eluting a part size exclusion chromatograph with 0.5mol / L of NaCl, further purifying, carrying out reduced pressure concentration on the above obtained 50kD or above component, and freeze-drying the obtained concentrate to obtain the Chinese angelica acidic polysaccharide component. The Chinese angelica acidic polysaccharide component has substantial cancer prevention, immunity enhancement and radiation damage prevention effects.

Description

technical field [0001] The invention relates to an angelica acidic polysaccharide component, an extraction method, and its application in the preparation of anti-cancer, immune-enhancing, and anti-radiation damage drugs, belonging to the field of traditional Chinese medicine modernization. Background technique [0002] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a famous and commonly used traditional Chinese medicine. Scholars at home and abroad have analyzed and studied the chemical components of Angelica Sinensis using modern extraction, separation and content determination methods, and found that the main active components of Angelica Sinensis are polysaccharides and volatile oils. In the past two decades, due to the development of molecular and cell biology, people have gradually realized that polysaccharides and their complexes have extremely important biological functions. Studies have found that Angelica polysaccharides have antithrombotic...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P35/02A61P37/04A61P39/00
Inventor 曹蔚李小强李卫燕王四旺周暄宣张雅
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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