Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of essential oil of alpinia oxyphylla miq. as penetration enhancer in fields of drug transdermal delivery and cosmetics

A technology for cosmetics and penetration enhancers, which can be used in cosmetic preparations, cosmetic preparations, pharmaceutical formulas, etc., and can solve problems such as toxicity and irritation that limit clinical applications.

Inactive Publication Date: 2016-10-05
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH +1
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the toxicity and irritation of some synthetic penetration enhancers (such as: dimethyl sulfoxide) limit their clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of essential oil of alpinia oxyphylla miq. as penetration enhancer in fields of drug transdermal delivery and cosmetics
  • Application of essential oil of alpinia oxyphylla miq. as penetration enhancer in fields of drug transdermal delivery and cosmetics
  • Application of essential oil of alpinia oxyphylla miq. as penetration enhancer in fields of drug transdermal delivery and cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Penetration Test

[0020] (1) Essential oil preparation:

[0021] a. Accurately weigh 300g of the pulverized fruit and leaf powder that has passed the No. 2 sieve, put it in a 5L round bottom flask, add 10 times the amount of water, and soak for 6 hours; heat the round bottom flask in a heating mantle to keep it slightly boiling , extracted by steam distillation for 6 hours; after stopping the heating, let it stand for a while, open the piston at the lower end of the tester to release the water slowly until the upper end of the oil layer reaches the 5mm mark; leave it for more than 1 hour, open the piston to lower the oil layer to its upper end Just flush with the scale 0, read the amount of volatile oil, and calculate the yield of volatile oil;

[0022] b. Essential oil storage: put the extracted essential oil in a centrifuge tube, add an appropriate amount of anhydrous sodium sulfate to dry; place in a centrifuge at 13,000 rpm, and centrifuge for 5 minutes...

Embodiment 2

[0034] Example 2: GC-MS Analysis of Components of Fruit and Leaf Essential Oils

[0035] 1. Sample treatment: Use steam distillation to obtain fruit and leaf essential oils respectively, accurately measure 10 μL of essential oils, put them in a 1 mL volumetric flask, dilute to the mark with ethyl acetate (chromatographic grade), and shake well.

[0036] 2. Chromatographic conditions: Thermo GC-MS gas chromatography-mass spectrometry instrument, AI / AS3000 sampling tower; carrier gas: nitrogen (purity 99.99%); GC column TR-5MS (30m×0.25mm i.d., 0.32μm); Injection port temperature: 250°C; sample volume: 1.0μL; splitless injection; temperature program, initial temperature 50°C, keep for 3min, increase temperature to 90°C at 6°C / min, hold for 5min, then increase temperature at 6°C / min Raise the temperature to 140°C, then raise the temperature to 145°C at 0.2°C / min, keep for 2min, then raise the temperature to 260°C at 15°C / min, and keep for 6min. Ionization mode (EI), scan time 0....

Embodiment 3

[0038] Example 3: Safety test of fruit essential oil and leaf essential oil on rabbit skin

[0039] 1. Select healthy New Zealand big-eared white rabbits, four in each group, half male and half male, and depilate on both sides of the back 24 hours before the experiment. The depilatory agent is 8g sodium sulfide, and 7g starch is dissolved in 100mL water (short the hair first, and then depilate) agent 2-3min), the range of hair removal is 3×3cm 2 , After hair removal, wash the hair removal area with an appropriate amount of warm water.

[0040]2. Take clean water as the blank control, dilute the base oil of the essential oil as the negative control, use 0.5mL each time, wash it with warm water four hours after applying it, and administer it to the same part continuously within a week, with the same administration time each time, observe Experimental results.

[0041] The results of the skin irritation test are shown in Fig. Figure 4

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

According to the invention, essential oil of alpinia oxyphylla miq. is taken as a major research object, and the effect of the essential oil on promoting drug transdermal permeation is investigated. On the basis of systematic investigation, both fruit essential oil and leaf essential oil of the alpinia oxyphylla miq. are relatively strong in penetration enhancing effect; and when the concentration of the essential oil is 3%, the penetration enhancing effect of the fruit essential oil and the leaf essential oil on drugs is obviously better than that of a common skin penetration enhancer, namely azone. Based upon GC-MS analysis, as for major ingredients, the fruit essential oil and the leaf essential oil are different in contents of alkane terpene and oxy-terpene ingredients; therefore, the fruit essential oil and the leaf essential oil keep a certain difference in drug penetration enhancing effect. In addition, the result of a skin irritation test proves that the fruit essential oil and the leaf essential oil, at an appropriate concentration, can avoid any erythema or edema in animal skin. Therefore, the essential oil of the alpinia oxyphylla miq., with the significant penetration enhancing effect and safety to the skin, has a broad development and application prospect in the fields of drug delivery and cosmetics.

Description

technical field [0001] The present invention relates to a method for researching the essential oil of the medicinal material Nootropia in Hainan as a skin penetration enhancer. The steam distillation method is used to extract the essential oils of the fruit and the leaf, and the isolated skin on the back of healthy rats is used as the penetration barrier. Indole Mexin was used as the model of the stimulated drug, the in vitro Franz diffusion cell was used as the osmosis device, UPLC-PDA was used as the detection technology for content analysis, GC-MS was used to analyze and compare the components of the two essential oils, and New Zealand big-eared white rabbits were used to conduct a skin irritation test to investigate the essential oils safety, which belongs to the technical field of medicine and cosmetics. Background technique [0002] The barrier function of the stratum corneum of the skin makes the transdermal penetration rate of most drugs difficult to meet the treatme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A61K8/97A61K31/405
Inventor 杨美华冯欢欢赵祥升魏建和
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com