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HPLC fingerprint spectrum of pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and building method and application of HPLC fingerprint spectrum

A technology of kudzu polyphylla and fingerprint spectrum, which is applied in the field of HPLC fingerprint spectrum of the medicinal material of kudzu puerariae or total flavonoids of pueraria juntae and its establishment field, and achieves the effects of good stability and reproducibility.

Active Publication Date: 2016-09-21
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of an accurate method to detect the authenticity and quality of the medicinal materials of Pueraria radiata and the quality of the total flavonoids of Pueraria variegata

Method used

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  • HPLC fingerprint spectrum of pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and building method and application of HPLC fingerprint spectrum
  • HPLC fingerprint spectrum of pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and building method and application of HPLC fingerprint spectrum
  • HPLC fingerprint spectrum of pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and building method and application of HPLC fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Establishment of HPLC Fingerprints of Pueraria multibranches or Total Flavonoids of Pueraria multibranches

[0053] 1. Instruments and reagents

[0054] Waters SYNAPT G2-S Q-TOF mass spectrometer (Waters, USA)

[0055] ACQUITY UPLC ultra-high performance liquid chromatography (American waters company)

[0056] ACQUITY UPLC BEH SHIELD RP18 Column (21mm×150mm, 1.7μm)

[0057] AY120 Tray Electronic Analytical Balance (Shimadzu Corporation, Japan)

[0058] DK-S24 Electric Heating Constant Temperature Water Bath (Shanghai Jinghong Experimental Equipment Co., Ltd.)

[0059] LABOROTA4000 rotary evaporator (Heidolph, Germany).

[0060] Acetonitrile is chromatographically pure (Opsen brand), liquid phase water is Watsons distilled water, ethanol is technical ethanol, and other reagents are analytically pure.

[0061] The medicinal materials of Pueraria microphylla were purchased from Yulin, Guangxi, and were identified as Pouzolzia zeylanica (L.) Benn.var.microphy...

Embodiment 2

[0076] Example 2 Identification of characteristic peaks of HPLC fingerprints of kudzu medicinal material or total flavonoids of kudzu

[0077] For the preparation of the test solution, refer to Item 2.1 in Example 1.

[0078] Chromatographic conditions refer to Item 2.2 in Example 1.

[0079] Preparation of reference solution: Accurately weigh 6,7-dimethyl-1,4-dihydro-2,3-quinoxalinedione, apigenin-6-C-a-L-arabinose 8-C-β-D- Glucoside, apigenin-6-C-[α-L-arabinose (1→6)-β-D-glucose], kaempferol-3-O-[α-L-rhamnose (1→6) -β-D-glucose]-7-O-α-L-glucoside, quercetin-3-O-[α-L-rhamnose (1→6)-β-D-glucose]-7- O-α-L-rhamnoside, quercetin-3,7-2-O-α-L-rhamnopyranoside, kaempferol-3-O-[α-L-rhamnose (1→ 6) An appropriate amount of -β-D-glucose]-7-O-α-L-rhamnoside and kaempferol-3,7-2-O-α-L-rhamnopyranoside reference substance is dissolved in an appropriate amount of methanol Configured as a reference solution, that is, too.

[0080] Mass spectrometry detection conditions: electrospray io...

Embodiment 3

[0085] Example 3 The method of using standard fingerprints to detect the authenticity of the medicinal material of Pueraria japonica

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Abstract

The invention relates to the technical field of detection of a traditional Chinese medicinal material, and particularly discloses an HPLC fingerprint spectrum of a pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and a building method and application of the HPLC fingerprint spectrum. The building method comprises the following steps: S1, precisely weighing a pouzolzia zeylanica var. microphylla medicinal material to prepare a to-be-tested solution; and S2, enabling the to-be-tested solution to be subjected to high performance liquid chromatography to obtain a fingerprint spectrum. The fingerprint spectrum can overall reflect information of characteristic peaks of a reaction sample, and the method is good in stability and reproducibility. The fingerprint spectrum can be used for true and false or quality identification of the pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicinal materials, in particular to an HPLC fingerprint spectrum of a medicinal material or total flavonoids of Pueraria japonica and its establishment method and application. Background technique [0002] Multibranch Mishuige (scientific name: Pouzolzia zeylanica var. microphylla) is a variety of Urticaceae Mishuige. Widely distributed in tropical Asia, produced in southeastern Yunnan, Guangxi, Guangdong, Fujian, southern Jiangxi, and Taiwan; it has detoxification and anti-inflammatory effects; it is used to treat boils and sores. Flavonoids are one of the active components of Pueraria twigs. At present, there is a lack of an accurate method for detecting the authenticity and quality of the medicinal materials of Pueraria radiata and the quality of the total flavonoids of Pueraria variegata. [0003] Traditional Chinese medicine fingerprint refers to the spectrum of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/06
CPCG01N30/06G01N30/8686
Inventor 郭丽冰陶曙红陈卓瀚
Owner GUANGDONG PHARMA UNIV
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