Micro-fluidic chip for single-cell isolation

Abstract

The invention discloses a micro-fluidic chip for single-cell isolation. The micro-fluidic chip comprises a substrate and a micro-channel formed in the substrate, wherein the micro-channel comprises a cell sample feeding hole, a single-cell collecting pool, a cell separation unit and a liquid drop output channel, the cell separation unit and the liquid drop output channel are sequentially communicated between the cell sample feeding hole and the single-cell collecting pool, a liquid drop generation packaging unit is formed at the joint of the cell separation unit and the liquid drop output channel and is communicated with an oil phase conveying channel, and the cell separation unit is used for conveying cells arrayed in a single-row manner to the liquid drop generation packaging unit. According to the micro-fluidic chip, a cell solution is treated by virtue of a spiral disc type micro-channel, so that the cells can be arrayed in a pipeline in a single-row manner and can be singly packaged by virtue of the liquid drop generation packaging unit.

Description

technical field [0001] The application belongs to the field of microfluidic technology, relates to single cell detection technology, and is a simple and practical microfluidic chip system for encapsulating single cells with droplets. Background technique [0002] The study of single cells is of great significance in the early diagnosis and treatment of major diseases, drug screening and research on cell physiological and pathological processes. With the progress of the research on stem cells and tumor cells, the research based on the single cell level has been paid more and more attention and has become a research trend. Single-cell isolation technology is the basis for research at the single-cell level. How to obtain a sufficient number of single cells is the key to research. [0003] At present, the method that can achieve single cell sorting is mainly flow cytometry, which can realize multi-channel sorting of living cells and lay the foundation for single cell research....

AI Technical Summary

Problems solved by technology

However, flow cytometers are expensive, bulky, and require special personnel to operate, so they cannot be popularized in large areas.

In addition, there are cell electrophoresis and limiting dilution methods to obtain single cells. Cell electrophoresis is based on the different charges of various cells or the same cells in different physiological states, so the swimming in a certain electric field Sorting is achieved based on the principle of different speeds, which is not suitable for the sorting of the same kind of cells in the same physiological state; the limited dilution method is simple to operate, but in actual operation, the efficiency is not high, generally only 20%, and requires the experimenter to observe and exclude, which is time-consuming strenuous

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