Hybrid snakehead spermatozoa preserving fluid and application thereof
A technology for preserving liquid and sperm, applied in the field of fish farming, can solve the problems of sperm inactivation and asynchrony of gonad development, and achieve the effects of long storage life, good sperm motility, and easy operation.
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Embodiment 1
[0017] Accurately weigh 8.2 g of sodium chloride, 0.47 g of potassium chloride, 0.128 g of calcium chloride, 0.4 g of sodium bicarbonate, and 10 g of glucose, dissolve them in 1 liter of distilled water, and prepare a sperm preservation solution (total osmotic pressure of 343 mOsm / kg, pH 7.4).
Embodiment 2
[0019] Accurately weigh 8.5g of sodium chloride, 0.5g of potassium chloride, 0.15g of calcium chloride, 0.6g of sodium bicarbonate, and 14g of glucose, dissolve them in 1 liter of distilled water, and prepare a sperm preservation solution (the total osmotic pressure is 380mOsm / kg , adjust the pH to 7.4).
Embodiment 3
[0026] Example 3: Sperm motility assay
[0027] The hybrid snakehead spermatozoa and preservation solution were diluted at a ratio of 1:9, and stored at 4°C. After the sperm were preserved in the preservation solution at 4°C for 96 hours, their viability and movement time were measured.
[0028] Determination of sperm motility in Examples and Comparative Examples: 0.5 ml of diluted semen was added dropwise on a common glass slide, 0.5 ml of water was added to activate, and the motility was immediately observed under a 10×16 magnification microscope. The results are shown in Table 1:
[0029] Table 1
[0030] Fast motion time (s) Total life (s) Activation rate (%) Example 1 177.4 206.3 94.7 Example 2 152.7 189.4 90.4 Comparative example 1 127.3 140.2 83.7 Comparative example 2 135.6 167.8 85.9 Comparative example 3 110.8 130.5 81.4
[0031] As can be seen from the results in Table 1, the sperm preservation solut...
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