Method for rapidly propagating fraxinus mandshurica by utilizing suspension culture technology

Abstract

The invention discloses a method for rapidly propagating fraxinus mandshurica by utilizing a suspension culture technology. The method comprises the steps of selecting seeds of the fraxinus mandshurica, soaking the selected seeds into an alcohol solution, washing with sterile water, putting the seeds into a sodium hypochlorite solution for sterilizing, and then washing with the sterile water again; taking out mature embryos, and inoculating into a seed germination medium for culturing; after 7-10 days, transferring into a liquid proliferation medium for culturing for 15-20 days; cutting off lateral buds, and continuously transferring into the liquid proliferation medium for culturing; carrying out acclimation on strong seedlings for a week, and then transplanting into a matrix treated by sterilization until the seedlings survive. The method has the advantages that the practical technological method for inducing the fraxinus mandshurica to rapidly propagate by utilizing the suspension culture method is simple and convenient, a production bottleneck caused by low seed germinate rate is broken, and culture period is shortened, thus improving propagation coefficient, and reducing excessive consumption of manpower and material resources in a tissue culture experiment process; therefore, the working efficiency is fundamentally improved, the production cost is lowered, and the aim of industrialized seedling production is achieved.

Description

technical field [0001] The invention relates to a method for rapidly propagating mandshurica mandshurica seedlings by means of suspension culture technology, and belongs to the technical field of tree tissue culture and breeding. Background technique [0002] Fraxinus mandschurica Rupr. Oleaceae, Ash genus, tall deciduous tree. It takes 15 to 20 years to bloom and bear fruit under the natural growth environment, and the fruit growing period is from September to October. Mandshurica mandshurica is an ancient relic plant with a wide distribution area, but most of them are scattered. It is one of the precious trees known as the "three hard broads" in Northeast my country. The wood material is fine and the texture is beautiful. It is widely used in various fields. Due to the early development history of Mandshurica mandshurica, its natural forest resources have become less and less in recent years. Due to excessive logging, its number has been decreasing day by day. At present,...

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Problems solved by technology

Most of the above-mentioned research materials choose axillary buds and tender stem tips, and the tree shape of Mandshurica mandshurica is tall, so the material has great limitations, and the pollution control of microorganisms inside Mandshurica mandshurica is difficult, so even if the multiplication culture is carried out, the multiplication will fail due to pollution. In Zhang Huijun's research, immature embryos were selected, but the differentiation rate was low during subculture differentiation, which did not meet the requirements for rapid propagation of seedlings in tissue culture.

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