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Preparation method and application of immunosensor based on Au-GQD@PtPd

An immunosensor, au-gqd technology, used in instruments, scientific instruments, measuring devices, etc., can solve the problems of cumbersome detection methods, high costs, and high detection limits, and achieve fast detection speed, avoid detection errors, and wide detection range. Effect

Inactive Publication Date: 2016-09-07
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for carcinoembryonic antigen. For example, the immunoassay methods commonly used to detect carcinoembryonic antigen mainly include radioimmunoassay, enzyme-linked immunoassay, fluorescence immunoassay and electrochemiluminescence immunoassay. , but most detection methods are cumbersome, complicated to operate, expensive, and have high detection limits. Therefore, it is of great significance to establish a fast, simple and sensitive detection method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. A method for preparing an immunosensor based on Au-GQD@PtPd, characterized in that the steps are as follows:

[0040] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0041] (2) HAuCl 4 (1 wt%) was electrodeposited onto the electrode surface from -0.2V by chronoamperometry, dried, rinsed with ultrapure water, and dried;

[0042] (3) Drop-coat 6 µL, 8 µg / mL of carcinoembryonic antigen capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0043] (4) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 3 µL, 0.5 mg / mL bovine serum albumin BSA solution on the electrode surface, 4 o C to dry in the refrigerator;

[0044] (5) After washing away unbound BSA with ultrapure water, add 6 µL, 1×10 -6 A series of human immunoglobulin solutions with different concentrations of ~ 10 ng / mL, incubated at room ...

Embodiment 2

[0046] Example 2. A method for preparing an immunosensor based on Au-GQD@PtPd, characterized in that the steps are as follows:

[0047] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0048] (2) HAuCl 4 (1 wt%) was electrodeposited onto the electrode surface from 0 V by chronoamperometry, dried in the air, rinsed with ultrapure water, and dried in the air;

[0049] (3) Drop-coat 6 µL, 10 µg / mL carcinoembryonic antigen capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0050] (4) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 3 µL, 1.0 mg / mL bovine serum albumin BSA solution on the electrode surface, 4 o C to dry in the refrigerator;

[0051] (5) After washing away unbound BSA with ultrapure water, add 6 µL, 1×10 -6 A series of human immunoglobulin solutions with different concentrations of ~ 10 ng / mL, ...

Embodiment 3

[0053] Example 3. A method for preparing an immunosensor based on Au-GQD@PtPd, characterized in that the steps are as follows:

[0054] (1) A glassy carbon electrode with a diameter of 5 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0055] (2) HAuCl 4 (1 wt%) was electrodeposited onto the electrode surface from 0.2V by chronoamperometry, dried, rinsed with ultrapure water, and dried;

[0056] (3) Drop-coat 6 µL, 12 µg / mL carcinoembryonic antigen capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0057] (4) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 3 µL, 1.5 mg / mL bovine serum albumin BSA solution on the electrode surface, 4 o C to dry in the refrigerator;

[0058] (5) After washing away unbound BSA with ultrapure water, add 6 µL, 1×10 -6 A series of human immunoglobulin solutions with different concentrations of ~ 10 ng / mL, incubated at room tem...

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Abstract

The invention belongs to the technical field of nano functional materials, immunoassay and biosensing, and provides a preparation method and application of an immunosensor based on Au-GQD@PtPd. A sandwich type electrochemical immunosensor for carcino-embryonic antigens is prepared by taking Au-GQD@PtPd as a detection antibody marker; due to excellent biocompatibility and high catalysis property of Au-GQD@PtPd, the prepared sensor has the advantages of high specificity, high sensitivity, low detection limit and the like.

Description

technical field [0001] The invention relates to a preparation method and application of an Au-GQD@PtPd-based immunosensor. Specifically, Au-GQD@PtPd is used as a detection antibody marker to prepare a sandwich type electrochemical immunosensor, which belongs to the technical fields of new functional materials, immune analysis and biosensing detection. Background technique [0002] Human beings are plagued by many diseases, such as lung cancer, ovarian cancer, breast cancer and cystadenocarcinoma, with high incidence, fast growth and metastasis, and great harm to human health. Carcinoembryonic antigen was originally found in colon cancer and fetal intestinal tissue. The increase of carcinoembryonic antigen content in human serum indicates that the human body is in an unhealthy state. The significance of carcinoembryonic antigen is that it can reflect the existence of a variety of tumors, and can be used to judge the curative effect, disease development, monitoring and progno...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N33/574
CPCG01N27/308G01N27/3278G01N33/57473
Inventor 刘青杨玉莹董云会刘会王平李月云
Owner SHANDONG UNIV OF TECH
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