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A kind of preparation method of copper ion chelating type nano particle biological coating

A nanoparticle and bio-coating technology, applied in coatings, polyamide coatings, hyaluronic acid coatings, etc., can solve problems such as poor blood compatibility, and achieve remarkable effects, wide application range, and strong controllability

Inactive Publication Date: 2018-03-23
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the poor hemocompatibility of 316L stainless steel and no inhibitory effect on vascular intimal hyperplasia and inflammation are the main aspects restricting its application.
At present, there is no relevant report on the use of copper ion chelated PLL / CS nanoparticles as a biological coating for the biochemical modification of the surface of 316L stainless steel materials.

Method used

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  • A kind of preparation method of copper ion chelating type nano particle biological coating
  • A kind of preparation method of copper ion chelating type nano particle biological coating
  • A kind of preparation method of copper ion chelating type nano particle biological coating

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Embodiment 1

[0025] see figure 1 , the first specific embodiment of the present invention is, a kind of preparation method of copper ion chelating nanoparticle biological coating, and its steps are:

[0026] A, preparation of copper ion chelated nanoparticles: CuCl with a concentration of 0.1mM 2 The aqueous solution was mixed with a polylysine (PLL, molecular weight 150-300 KDa) solution with a concentration of 2.0 mg / ml in equal volumes, and allowed to stand at room temperature for 1 hour. Then the above-mentioned composite liquid is mixed with the chondroitin sulfate solution whose concentration is 10 mg / ml in equal volumes to obtain copper ion chelated nanoparticles;

[0027] B. Deposition of polydopamine coating: Deposit polydopamine coating on the surface of 316L medical stainless steel, and heat treatment at 60°C for 12 hours; on the one hand, dry the sample, and on the other hand, accelerate the conversion of phenolic hydroxyl group to quinone group in the dopamine coating transf...

Embodiment 2

[0030] A preparation method of a copper ion chelating nanoparticle biological coating, the steps of which are:

[0031] A, preparation of copper ion chelated nanoparticles: CuCl with a concentration of 10.0mM 2 The aqueous solution was mixed with a polylysine (PLL, molecular weight 150-300KDa) solution with a concentration of 5.0 mg / ml in equal volumes, and allowed to stand at room temperature for 3 hours. Then the above-mentioned composite solution is mixed with the chondroitin sulfate solution whose concentration is 50 mg / ml in equal volumes to obtain copper ion chelated nanoparticles;

[0032] B. Deposition of polydopamine coating: deposit polydopamine coating on the surface of 316L medical stainless steel, and heat treatment at 60°C for 12 hours;

[0033] C. Preparation of nanoparticle biological coating: Soak the sample deposited with polydopamine coating in step B in the nanoparticle suspension obtained in step A, shake and react at 40°C for 6 hours, rinse with double d...

Embodiment 3

[0035] A preparation method of a copper ion chelating nanoparticle biological coating, the steps of which are:

[0036] A, the preparation of copper ion chelating type nanoparticle: the CuCl that concentration is 5mM Aqueous solution and the polylysine (PLL, molecular weight 150-300KDa) solution equivolume mixing that concentration is 3.0mg / ml, leave standstill under room temperature 2 Hour. Then the above-mentioned composite liquid is mixed with the chondroitin sulfate solution whose concentration is 25 mg / ml in equal volumes to obtain copper ion chelated nanoparticles;

[0037] B. Deposition of polydopamine coating: deposit polydopamine coating on the surface of 316L medical stainless steel, and heat treatment at 60°C for 12 hours;

[0038] C. Preparation of nanoparticle biological coating: Soak the sample deposited with polydopamine coating in step B in the nanoparticle suspension obtained in step A, shake and react at 30°C for 12 hours, and rinse with double distilled wat...

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Abstract

The invention discloses a preparation method of a copper ion chelated nano particle biological coating. The preparation method comprises the following steps: at first, chelating PLL and copper ions to obtain a PLL / copper ion compound; utilizing the characteristic that PLL and chondroitin sulfate can carry out electrostatic interaction to mix PLL / copper ion compound with chondroitin sulfate so as to form copper ion chelated nano ions; then preparing a DM coating on the surface of 316L stainless steel, utilizing the characteristic that DM and primary amino group can carry out Schiff base reactions to fix nano particles containing amino groups onto the surface of a sample so as to establish a multifunctional surface that can prevent coagulation and proliferation and promote endothelial regeneration. By establishing a nano particle coating, which has multiple biological functions, on the surface f 316L stainless steel, the anti-coagulation and anti-proliferation functions of stainless steel are prominently enhanced, and at the same time, the performance of repairing endothelial injury is strengthened.

Description

technical field [0001] The invention relates to nanoparticle preparation technology and inorganic material surface modification technology, in particular to a biological modification method for the surface of stainless steel-based cardiovascular implant materials. Background technique [0002] 316L stainless steel has good mechanical properties and excellent corrosion resistance, and is widely used in the field of biomedical materials such as bone materials, dental implants, and cardiovascular implant materials. However, for some special application areas, such as the treatment of coronary heart disease as a stent material, its safety and biocompatibility are far from meeting the clinical requirements. Among them, the poor hemocompatibility of 316L stainless steel and its non-inhibitory effect on vascular intimal hyperplasia and inflammation are the main aspects restricting its application. [0003] By biochemically modifying the material surface, constructing a reasonable ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D179/00C09D105/08C09D177/04C09D7/61A61L27/28A61L27/54A61L31/08A61L31/16
CPCA61L27/28A61L27/54A61L31/08A61L31/16A61L2300/102A61L2300/412A61L2300/416A61L2300/602A61L2300/608A61L2420/04A61L2420/08C09D7/61C09D105/08C09D179/00C08L77/04C08K3/16
Inventor 刘涛曾峥刘诗卉潘长江陈俊英丁红燕
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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