Yeast microcapsules of artichoke polyphenols and preparation method thereof
A technology of microcapsules and artichokes, applied in the field of medicine, can solve the problems of expensive equipment, residual chemical reagents, etc., and achieve the effects of convenient application, easy preservation and wide source.
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Embodiment 1
[0024] 1) Take 2000 g of artichoke stems and leaves, pulverize them with a traditional Chinese medicine grinder, and pass through a 60-mesh sieve to obtain artichoke stems and leaves powder, add 40 times the amount of 95% ethanol, and extract 3 times with 800W ultrasonic waves, each time for 180 minutes; filter, combine the filtrates, Concentrate to obtain a clear paste with a relative density of 1.25 to 1.46 at 50°C, freeze-dry to obtain artichoke polyphenol freeze-dried powder;
[0025] 2) Take the commercially available Angel dry yeast, add 20 times the amount of 5% NaCl solution, shake in a water bath at 54°C for 5 hours, centrifuge at 5000rpm for 10 minutes, wash twice with water, and freeze-dry to complete the pretreatment of yeast cells;
[0026] 3) Mix the artichoke polyphenol freeze-dried powder obtained in step 1) with the pretreated yeast cells obtained in step 2) according to the ratio of core wall to 1:4, add 20 times the amount of distilled water, and at 70°C, Af...
Embodiment 2
[0029] 1) Take 1000 g of artichoke stems and leaves, pulverize them with a traditional Chinese medicine grinder, and pass through a 20-mesh sieve to obtain artichoke stems and leaves powder, add 40 times the amount of 95% ethanol, and extract 3 times with 400W ultrasonic waves, each time for 120 minutes; filter, combine the filtrates, Concentrate to obtain a clear paste with a relative density of 1.25 to 1.46 at 50°C, freeze-dry to obtain artichoke polyphenol freeze-dried powder;
[0030] 2) Take the commercially available Angel dry yeast, add 20 times the amount of 5% NaCl solution, shake in a water bath at 54°C for 5 hours, centrifuge at 5000rpm for 10 minutes, wash twice with water, and freeze-dry to complete the pretreatment of yeast cells;
[0031] 3) Mix the artichoke polyphenol freeze-dried powder obtained in step 1) with the pretreated yeast cells obtained in step 2) according to the ratio of core wall to 1:4, add 20 times the amount of distilled water, and at 60°C, Af...
Embodiment 3
[0033] 1) Take 1500 g of artichoke stems and leaves, pulverize them with a traditional Chinese medicine grinder, and pass through a 60-mesh sieve to obtain artichoke stems and leaves powder, add 40 times the amount of 95% ethanol, and extract 3 times with 800W ultrasonic waves, each time for 180min; filter, combine the filtrates, Concentrate to obtain a clear paste with a relative density of 1.25 to 1.46 at 50°C, freeze-dry to obtain artichoke polyphenol freeze-dried powder;
[0034] 2) Take the commercially available Angel dry yeast or the waste yeast recovered from the fermentation factory, add 20 times the amount of 5% NaCl solution, shake in a water bath at 54°C for 5 hours, centrifuge at 5000rpm for 10 minutes, wash twice with water, and freeze-dry to complete the pretreatment of yeast cells ;
[0035]3) Mix the artichoke polyphenol freeze-dried powder obtained in step 1) with the pretreated yeast cells obtained in step 2) according to the ratio of core wall to 1:1, add 2...
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