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Molecular marker detecting ischemic cerebralvascular accident and application thereof

A technology of ischemic stroke and molecular markers, applied in the field of biomedicine, can solve the problem of lack of molecular markers

Inactive Publication Date: 2016-08-31
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, molecular markers in cerebrovascular disease are still relatively lacking

Method used

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  • Molecular marker detecting ischemic cerebralvascular accident and application thereof
  • Molecular marker detecting ischemic cerebralvascular accident and application thereof
  • Molecular marker detecting ischemic cerebralvascular accident and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The collection of embodiment 1 sample

[0055] From June 2015 to December 2015, patients with ischemic stroke who were treated in PLA Rocket Army General Hospital were collected. A total of 6 cases were collected in the case group. A total of 6 healthy volunteers were collected, and the specific information is shown in Table 1. Obtain 5ml of cubital median venous blood from all research subjects, put it in a 4-degree refrigerator for 1 hour, centrifuge at 1500 rpm for 10 minutes, take the supernatant, centrifuge at 1500 rpm for 10 minutes, take the supernatant, and store in a -80-degree refrigerator.

[0056] Table 1 Sample information

[0057] factor

Embodiment 2

[0058] Example 2 iTRAQ experiment screening differential proteins

[0059] 1. Instruments and reagents used

[0060] Vortex oscillator (Haimen Qilinbeier Instrument Manufacturing Co., Ltd., model: QL-901)

[0061] Constant temperature incubator (Shanghai Pudong Rongfeng Scientific Instrument Co., Ltd., model: HH.S4)

[0062] Vacuum freeze dryer (Thermo, model: SPD2010-230 )

[0063] RIGOL L-3000 HPLC system (Beijing Puyuan Jingdian Technology Co., Ltd.), mobile phase A: 98% ddH2O, 2% acetonitrile (pH 10); mobile phase B: 98% acetonitrile, 2% ddH 2O (pH 10) high performance liquid chromatography: (ThermoScientic EASY-nLC 1000 System (Nano HPLC)), mobile phase A: 100% ultrapure water, 0.1% formic acid; mobile phase B: 100% acetonitrile, 0.1% formic acid mass spectrometry System (Thermo, model: Q-Exactive )

[0064] Blue Albumin

[0065] IgG Removal Kit (Sigma-Aldrich, Cat. No.: PROTBA-1KT )

[0066] DTT (Bio-Rad, catalog number: 161-0611, USA )

[0067] Iodoacetamide (B...

Embodiment 3

[0133] Example 3 Large sample verification of expression of differential protein PIGR

[0134] (1) Dilution of the standard substance Add 1mL of the standard substance diluent to each bottle of the standard substance, cover it and let it stand at room temperature for about 10 minutes. Prepare 7 EP tubes of diluted standard, add 150 μL of standard diluent to each EP tube, dilute into different gradients, and directly use the standard diluent (0pg / mL) as a blank well.

[0135] (2) Adding samples: set up blank wells (blank control wells do not add samples and enzyme-labeled reagents, and the rest of the steps are the same), standard wells, and sample wells to be tested. Accurately add 50 μl of the standard substance on the enzyme-labeled plate, add 40 μl of the sample diluent to the well of the sample to be tested, and then add 10 μl of the sample to be tested (the final dilution of the sample is 5 times). Adding the sample Add the sample to the bottom of the well of the microti...

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Abstract

The invention discloses a molecular marker detecting ischemic cerebralvascular accident. The molecular marker comprises PIGR protein or active fragments thereof, mRNA encoding the PIGR protein, and gene encoding the PIGR protein. The PIGR protein relevant to cerebralvascular accident is disclosed, and it is further proved that expression the PIGR protein or active fragments thereof is up-regulated in cerebralvascular tissue. By detecting ischemic cerebralvascular accident with the protein, early detection can be conducted quickly and effectively, and treating targets and important basis are provided for clinical application including gene therapy and drug therapy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a molecular marker for detecting ischemic stroke and its application. Background technique [0002] Stroke is also called "stroke" or "cerebrovascular accident" (cerebral vascular accident, CVA). It is an acute cerebrovascular disease, which is a group of diseases that cause brain tissue damage due to the sudden rupture of blood vessels in the brain or the inability of blood to flow into the brain due to vascular obstruction, including ischemic and hemorrhagic stroke. [0003] Ischemic stroke, also known as cerebral infarction, is called stroke in traditional Chinese medicine. The disease is caused by a variety of causes of blood supply disturbance in the local brain tissue, leading to ischemic and hypoxic lesions and necrosis of the brain tissue, resulting in clinically corresponding neurological deficits. Ischemic stroke is divided into main types such as cerebral thrombosis, cereb...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6812G01N2333/78
Inventor 刘昊
Owner 北京致成生物医学科技有限公司
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