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Method for detecting concentration of D-tryptophan in solution

A tryptophan and solution technology, applied in the direction of material electrochemical variables, can solve the problems of high detection limit, low accuracy, low sensitivity, etc., to reduce detection cost, simple and convenient operation, and improve sensitivity and accuracy Effect

Active Publication Date: 2016-08-24
苏州盛泽科技创业园发展有限公司
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Problems solved by technology

[0004] Existing methods for detecting amino acids with different configurations include cyclic voltammetry and AC impedance. These methods only use one of the indicators as a basis for simultaneous qualitative and quantitative analysis. For example, cyclic voltammetry only uses the magnitude of the current signal as a method to distinguish different configurations. Amino acids, the AC impedance is distinguished by the resistance value, its accuracy is low, the sensitivity is low, and the detection limit is high

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  • Method for detecting concentration of D-tryptophan in solution
  • Method for detecting concentration of D-tryptophan in solution

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Embodiment 1

[0048] A method for detecting the concentration of D-tryptophan in a solution, comprising the following steps:

[0049] Step 1, preparing the working electrode and building a three-electrode system, preparing multiple standard solutions of D-type tryptophan with a concentration of 0-100 μmol / L;

[0050] Among them, the preparation method of the working electrode, that is, the modified glassy carbon electrode is: immerse the reference electrode, the auxiliary electrode and the pretreated glassy carbon electrode in a mixed solution containing 2 mg / mL nitrogen-doped carbon dots and 0.01 mg / mL β-cyclodextrin In the process, the modified glassy carbon electrode was obtained by taking out the cyclic voltammetry scan and drying it. The scan parameters were set as follows: initial potential 0V, highest potential 1V, lowest point 0V, final potential 0V, scan rate 0.1V / s, scan times 100 times, sensitivity 10 -4 A / V, waiting time is 2s;

[0051] Wherein, the standard solution configura...

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Abstract

The invention discloses a method for detecting the concentration of D-tryptophan in a solution. D-tryptophan in the solution to be detected is detected through differential pulse voltammetry with a three-electrode system, and the concentration of D-tryptophan in the solution to be detected is obtained according to a linear equation of D-tryptophan, wherein working electrodes in the three-electrode system are modified glassy carbon electrodes obtained by modifying glassy carbon electrodes with nitrogen-doped carbon dots and beta-cyclodetrin. By means of the method, the concentration of D-tryptophan can be rapidly detected; besides, sensitivity and accuracy are high.

Description

technical field [0001] The invention relates to the technical field of isomeric amino acid detection. More specifically, the present invention relates to a method for detecting the concentration of D-type tryptophan in solution by differential pulse voltammetry based on nitrogen-doped carbon dots and β-cyclodextrin modified electrochemical sensing. Background technique [0002] Tryptophan is an important precursor substance for auxin biosynthesis in plants. Its structure is similar to indole acetic acid, and it is ubiquitous in higher plants. Auxin can be synthesized from tryptophan. This product is an important nutritional agent. It can participate in the renewal of plasma proteins in animals, and can promote riboflavin to play a role. It can also help the synthesis of niacin and heme. It can significantly increase the antibodies in the fetuses of pregnant animals, and it can promote lactation for lactating cows and sows. effect. When livestock and poultry lack tryptoph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 肖琦黄珊卢双燕黄初升苏炜崔建国
Owner 苏州盛泽科技创业园发展有限公司
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