Method for preparing prunus armeniaca kernel antioxidative peptide through enzymolysis of prunus armeniaca kernels

Abstract

The invention discloses a method for preparing prunus armeniaca kernel antioxidative peptide through enzymolysis of prunus armeniaca kernels. Through the single-factor experiment and the response surface optimization experiment, the optimal technological conditions for preparing the prunus armeniaca kernel antioxidative peptide through the composite enzymolysis of alkaline protease and flavourzyme are determined as follows: the dosage of enzyme: 6725U / g, the substrate concentration: 3%, the enzymolysis temperature: 60 DEG C, the pH: 8.28, the three times of parallel tests prove that the average value of the clearance rate of the obtained enzymatic hydrolysate for OH is 84.18%, the average value of DH is 20.87%, the average value of reducing power is 0.743, meanwhile, a dialysis bag, the gel chromatography technology, the high performance liquid chromatography (HPLC) and the reversed phase high-performance liquid chromatograph (RP-HPLC) are adopted for carrying out further separation and purification on AAMH, and the matrix-assisted laser desorption tandem time-of-flight mass spectrometry technology and the Edman degradation method are adopted for identifying the structure of the obtained prunus armeniaca kernel antioxidative peptide.

Description

technical field [0001] The invention relates to a method for preparing antioxidative peptides from almonds, in particular to a method for preparing antioxidative peptides from almonds by enzymolyzing almonds. Background technique [0002] With the continuous development of nutrition and biotechnology, researchers have found that after protein is decomposed by digestive tract enzymes, free amino acids are not the main form of absorption, but are absorbed in the form of short peptides between amino acids and proteins. The body absorbs. Bioactive peptides are short peptides between amino acids and proteins. They have the characteristics of easy absorption and utilization by the human body, high safety, and low production costs, and act as potential physiological effectors of metabolism during intestinal digestion. In recent years, a large number of studies have shown that bioactive peptides can be prepared by hydrolyzing food-derived proteins with suitable proteases, and a cla...

AI Technical Summary

Problems solved by technology

The protein content of degreased almond meal is as high as 50%, but most of them are currently used as feed or made into almond meal with poor functionality, with low economic utilization value and product added value

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