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Preparation method of fluorescent probe and oxytetracycline detection method based on same

A technology of fluorescent probes and detection methods, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of long detection time, complex instruments, cumbersome steps, etc., and achieve strong anti-interference ability and good selectivity , synthesize simple effects

Inactive Publication Date: 2016-08-24
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods either have cumbersome steps, and most of the reagents used are some environmentally unfriendly reagents; or they need to use complex and expensive instruments and long detection time, so the practicability is poor

Method used

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  • Preparation method of fluorescent probe and oxytetracycline detection method based on same
  • Preparation method of fluorescent probe and oxytetracycline detection method based on same
  • Preparation method of fluorescent probe and oxytetracycline detection method based on same

Examples

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preparation example Construction

[0035] A method for preparing a fluorescent probe, comprising the following steps:

[0036] (1) Weigh pyrrole and glutaric anhydride and dissolve them in an appropriate amount of dichloromethane to obtain a mixture, and then quickly add the mixture to a three-necked flask, under N 2 Under protection, use a dropping funnel to add boron trifluoride etherate complex dropwise into a three-necked flask, fully stir at room temperature, and react for 8-9 hours;

[0037] The molar ratio of pyrrole, glutaric anhydride, and boron trifluoride etherate complex is 2:1:6-8;

[0038] (2) In an ice-bath environment, slowly add triethylamine into a three-necked flask with a dropping funnel, and stop the reaction after stirring for 3.5-4 hours;

[0039] The molar ratio of added triethylamine to boron trifluoride etherate complex is 1:1-1.25;

[0040] (3) the reaction solution that above-mentioned step obtains is extracted 3 times with the mixed solution of sherwood oil and dichloromethane, co...

Embodiment 1

[0049] A method for preparing a fluorescent probe, comprising the following steps:

[0050] (1) Weigh pyrrole and glutaric anhydride and dissolve them in an appropriate amount of dichloromethane to obtain a mixture, and then quickly add the mixture to a three-necked flask, under N 2 Under protection, add boron trifluoride etherate complex dropwise into the three-necked flask with a dropping funnel, fully stir at room temperature, and react for 8 hours;

[0051] The molar ratio of pyrrole, glutaric anhydride, and boron trifluoride etherate complex is 2:1:6;

[0052] (2) Under the environment of ice bath, slowly add triethylamine in the there-necked flask with dropping funnel, stop reaction after stirring for 3.5 hours;

[0053] The molar ratio of added triethylamine to boron trifluoride etherate complex is 1:1;

[0054] (3) the reaction solution that above-mentioned step obtains is extracted 3 times with the mixed solution of sherwood oil and dichloromethane, collects the big...

Embodiment 2

[0057] A method for preparing a fluorescent probe, comprising the following steps:

[0058] (1) Weigh pyrrole and glutaric anhydride and dissolve them in an appropriate amount of dichloromethane to obtain a mixture, and then quickly add the mixture to a three-necked flask, under N 2 Under protection, use a dropping funnel to add boron trifluoride etherate complex dropwise into a three-necked flask, fully stir at room temperature, and react for 9 hours;

[0059] The molar ratio of pyrrole, glutaric anhydride, and boron trifluoride etherate complex is 2:1:8;

[0060] (2) Under the environment of ice bath, slowly add triethylamine in the there-necked flask with dropping funnel, stop reaction after stirring for 4 hours;

[0061] The molar ratio of added triethylamine to boron trifluoride etherate complex is 1:1.25;

[0062] (3) the reaction solution that above-mentioned step obtains is extracted 3 times with the mixed solution of sherwood oil and dichloromethane, collects the bi...

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Abstract

The invention discloses a preparation method of a fluorescent probe and an oxytetracycline detection method based on the same. The oxytetracycline detection method includes that the fluorescent probe high in water solubility is synthesized by structurally modifying a fluorine-boron fluorescent dye master ring; on the basis that oxytetracycline has good and high-sensitivity fluorescence quenching effect on the probe, a linear formula is built according to changing values of fluorescent strength of the fluorescent probe and concentration of acid oxytetracycline before and after an oxytetracycline standard solution is added; the fluorescent strength of the probe before and after an oxytetracycline sample is added is detected in same conditions, and then content of oxytetracycline in the sample can be calculated according to changing of the fluorescent strength. Related toxic reagents are avoided being used, expensive instruments do not need to be used, detection time is shortened, detection sensitivity and specificity are improved, and the oxytetracycline detection method has good application prospect.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for preparing a fluorescent probe and a method for detecting oxytetracycline based on the fluorescent probe. Background technique [0002] Antibiotics are a class of special drugs that can be used to treat infections in human diseases, or as veterinary drugs to prevent bacterial infections in livestock and increase their growth rate. Oxytetlacycline (OTC), also known as oxytetracycline, dilimycin, oxytetracycline, tetracycline, etc., belongs to tetracycline antibiotics and is widely used in the treatment of infectious diseases in animals. In animal feed, it is often widely used as veterinary drug antibiotics and production accelerators, which has led to its accumulation in food, such as meat, milk and egg products, and has caused serious threats to human health. my country is a big country in the production and use of antibiotics. In 2003 alone, ...

Claims

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Application Information

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IPC IPC(8): C07F5/02C09K11/06C09B57/00G01N21/64
CPCC07F5/022C09B57/00C09K11/06C09K2211/104G01N21/6428
Inventor 许小平胥智群欧敏锐祝令辉谷俊杰
Owner FUZHOU UNIV
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