2385 polymorphism detection kit for LRRK2 gene

A technology of polymorphism detection and kit, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effects of simple operation, easy automation, and cost saving

Inactive Publication Date: 2016-08-17
XIAMEN RENRUI BIOMEDICAL TECH CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

At present, there is no report of using real-time fluorescent PCR method to study the 2385 polymorphism of LRRK2 gene

Method used

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  • 2385 polymorphism detection kit for LRRK2 gene
  • 2385 polymorphism detection kit for LRRK2 gene
  • 2385 polymorphism detection kit for LRRK2 gene

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0026] Example 1 Real-time fluorescent PCR detection of three types of LRRK22385 polymorphic sites (rs34778348).

[0027] 1. Materials

[0028] 1. Instruments: real-time fluorescent PCR instrument, pipette, centrifuge.

[0029]2. Design of primers and probes: The present invention designs primers that can specifically amplify the DNA fragment containing the 2385 polymorphic site of the LRRK2 gene, and a probe that specifically recognizes the 2385 polymorphic site of the LRRK2 gene. LRRK2 gene 2385 polymorphism site (rs34778348) c.7153 base is G labeled as FAM, LRRK2 gene 2385 polymorphic site (rs34778348) c.7153 base is A probe is labeled as HEX. Therefore, in the reaction tube where the c.7153 base is G / G as the template, the FAM channel should have an amplification signal, and the HEX channel should have no amplification signal; the c.7153 base is the A / A sample as the template. In the reaction tube, the HEX channel should have an amplification signal, and the FAM channel ...

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Abstract

The invention discloses a 2385 polymorphism detection kit for an LRRK2 gene, and relates to gene polymorphism detection. The 2385 polymorphism detection kit comprises a primer, a fluorescent probe and a reagent, wherein the primer comprises a DNA fragment which is for specific amplification and comprises 2385 sites of the LRRK2 gene; the base sequence of the primer is as shown in SEQ ID NO:1 and SEQ ID NO:2; the fluorescent probe is used for recognizing 2385 polymorphism of the LRRK2 gene; a fluorescent group is marked by the 5'-end of the fluorescent probe; a quenching group is marked by the 3'-end of the fluorescent probe; two nucleic acid probes for marking two different fluorescent group markers are adopted to indicate situation of two polymorphisms in one reaction tube simultaneously, and the base sequences of the polymorphisms are as shown in SEQ ID NO:3 and SEQ ID NO:4; the reagent comprises MgCl2, hot start Taq enzyme, UNG enzyme, dATP, dCTP, dGTP, dTTP, dUTP and a PCR reaction buffer liquid.

Description

technical field [0001] The invention relates to gene polymorphism detection, in particular to a LRRK2 gene 2385 polymorphism detection kit. Background technique [0002] Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease (AD). The prevalence of PD among people over 65 years old in my country is 1.17%, which is similar to developed countries in Europe and the United States. This ratio rises to 3% among people over 75 years old. Therefore, Parkinson's disease has become a serious threat to human health in modern society in my country. one of the major diseases. [0003] The average age of Parkinson's disease diagnosis is 70 years old, but its symptoms appear earlier than the clinical diagnosis. Therefore, finding early biological markers of Parkinson's disease and performing genetic diagnosis are extremely important for its risk prediction and later prevention. Parkinson's disease usually lasts 15 years from diagnosis to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 郭奇伟谢勇壮钟力许华曦卜国军曹甜甜
Owner XIAMEN RENRUI BIOMEDICAL TECH CO LTD
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