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Rhodobacter capsulatus strain as well as screening method and application thereof

A technology of capsular rhodobacteria and strains, applied in the field of bioengineering, can solve problems such as deterioration of aquaculture water bodies and frequent occurrence of aquaculture diseases

Active Publication Date: 2016-08-17
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve many problems that the existing high-density aquaculture model easily leads to frequent aquaculture diseases and serious deterioration of aquaculture water, the present invention provides a bacterial strain with antibacterial and digestive enzyme activity and its screening method and application. A novel strain isolated from the intestinal tract of Penaeus vannamei with antimicrobial activity and protease, amylase and lipase production activities

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  • Rhodobacter capsulatus strain as well as screening method and application thereof
  • Rhodobacter capsulatus strain as well as screening method and application thereof
  • Rhodobacter capsulatus strain as well as screening method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] The Litopenaeus vannamei used in this example was taken from Xinyongfeng Prawn Farm in Hangu District, Tianjin;

[0042] The 2216E medium used in this example was prepared with a nine-salt solution;

[0043] The 2216E medium used in this example includes the weight ratio of the following components: peptone 5g / L, yeast extract 1g / L, iron phosphate 0.1g / L, agar 20g / L pH7.6-7.8.

[0044] The indicator bacteria described in this example is Vibrio anguillarum, which was purchased from the Institute of Microbiology, Chinese Academy of Sciences and preserved in the Hydrobiology Laboratory of Tianjin Normal University.

[0045] The screening method of the Rhodobacter capsulatus R3 bacterial strain of the present embodiment:

[0046] Isolation and purification of bacterial strains: Under sterile conditions, take the whole intestine of prawns in a homogenizer, grind in an ice bath, and dilute the grinding liquid with a nine-salt solution in a gradient of 10-1 to 10-8; choose a ...

Embodiment 2

[0049] This embodiment has a screening method for the Rhodobacter capsulatus R3 strain that produces digestive enzyme activity, and its separation and purification method is the same as that in Example 1, and will not be repeated here.

[0050] The digestive enzyme activity test was carried out on the purified strain:

[0051] A. Transfer the purified strain to the liquid culture medium at 28°C for constant temperature shaking culture overnight, use sterile tweezers to pick up a piece of sterile filter paper and place it in the culture medium to soak the bacteria solution, and place it in the prepared skim milk medium At the corresponding position, culture at a constant temperature of 28°C for 24 hours, and screen the strains that can decompose proteins and produce transparent circles.

[0052] B. Transfer the purified strain to the liquid culture medium with constant temperature shaking at 28°C overnight, use sterile tweezers to pick up a piece of sterile filter paper and pla...

Embodiment 3

[0058] The Rhodobactercapsulatus R3 strain obtained in the above-mentioned examples with antibacterial activity and digestive enzyme activity was selected for identification and analysis. The specific identification and analysis methods and corresponding identification results are as follows.

[0059]The Rhodobactercapsulatus R3 strain with antibacterial activity and digestive enzyme activity screened by the method of the above-mentioned examples was inoculated on a 2216E medium plate, cultured upside down at 28°C for 24 hours, and the growth state, strain morphology and physiology of the colonies were observed. The biochemical test refers to the technical manual of industrial microbiological test (Zhuge Jian, Wang Zhengxiang, technical manual of industrial microbiological test [M], China Light Industry Press, 1994) and Berger's bacterial identification manual (R.E Buchanan, N.E Gibbons, Burger Jay's Bacteria Identification Manual [M], Science Press, Beijing, 1984). Observe it...

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Abstract

The invention discloses a screening method of rhodobacter capsulatus and an application thereof. The collection register number of the rhodobacter capsulatus is CGMCC No:11753. In the invention, the rhodobacter capsulatus is used as a feed additive for preparing a prawn feed; the feeding result indicates that the growth speed of the prawns eating the feed added with the rhodobacter capsulatus is remarkably increased in comparison with that the prawns eating common feed while the immunity is obviously improved; a method for preparing the feed by adopting the rhodobacter capsulatus has the advantages that production cycle is short, production cost is low, growth conditions are easy to control, antibacterial activity is high, immunity is obviously improved and the like; and moreover, the rhodobacter capsulatus totally has potential for industrial production and gets relatively good production and application prospect.

Description

[0001] The present invention is funded by the 973 project "Immunological approaches and key technical principles for the prevention and treatment of prawn white spot syndrome", project number 2012CB114405, and Tianjin Normal University Biology Outstanding Young Teacher Funding Project, project number ZX110QN008. technical field [0002] The invention relates to a capsulated rhodobacter strain and its screening method and application, belonging to the technical field of bioengineering. Background technique [0003] Litopenaeus vannamei (Litopenaeus vannamei), also known as White Prawn, Litopenaeus vannamei is delicious in meat, and it is also known as the world's three major shrimps together with Chinese prawn and Penaeus monodon. In the shrimp farming industry of our country play an extremely important role. However, with the development of intensive culture of Litopenaeus vannamei, the outbreak of prawn diseases has caused serious harm to the culture of Litopenaeus vannamei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/52C12N9/26C12N9/20A23K10/18A23K50/80C02F3/34A61P31/04C12R1/01
CPCC02F3/34C12N9/20C12N9/2411C12N9/52C12N1/205C12R2001/01
Inventor 左志晗孙金生窦春萌
Owner TIANJIN NORMAL UNIVERSITY
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