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Phaffia rhodozyma strain rich in astaxanthin and screening method and application of Phaffia rhodozyma strain

A technology of Phaffia rhodozyma and yeast strains, applied in the field of astaxanthin-rich Phaffia rhodozyme strains and its screening, to achieve the effects of fast production speed, easy high-density fermentation, and no need for light

Inactive Publication Date: 2016-08-17
吉林省希玛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Astaxanthin production using Phaffia rhodozyma is still at the basic stage of experimental research

Method used

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  • Phaffia rhodozyma strain rich in astaxanthin and screening method and application of Phaffia rhodozyma strain
  • Phaffia rhodozyma strain rich in astaxanthin and screening method and application of Phaffia rhodozyma strain
  • Phaffia rhodozyma strain rich in astaxanthin and screening method and application of Phaffia rhodozyma strain

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Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1 determines the screening temperature of Phaffia rhodozyme strain

[0045] Place the plates coated with the same concentration of mixed bacterial solution in incubators at 18°C, 16°C, 14°C, 12°C, and 10°C, respectively, and observe the time it takes for Phaffia rhodozyma colonies to grow into red and obvious colonies. The number of colonies of miscellaneous bacteria other than Phaffia rhodozyma. The results are shown in the table below:

[0046] Culture temperature 18℃ 16℃ 14℃ 12℃ 10℃ Phaffia rhodozyma formation time 3 days 4 days 5 days 7 days 10 days The number of bacteria colonies +++++ +++++ ++++ +++ ++

[0047] Phaffia rhodozyme has the characteristic of low temperature resistance. Using low temperature to screen Phaffia rhodozyme can effectively inhibit the growth of miscellaneous bacteria, making it easier to isolate Phaffia rhodozyme on the medium, which is beneficial to the large-scale screening of Phaffi...

Embodiment 2

[0048] Example 2 Low temperature culture method screening and isolation of Phaffia rhodozyma strain XM-1601 (Phaffia rhodozyma XM-1601) of the present invention

[0049] Step 1, the leaf layer with Phaffia rhodozyme is pretreated: prepare sterilized physiological saline and a beaker, then repeatedly soak the leaf layer with Phaffia rhodozyme in the beaker with sterilized physiological saline, Use a 0.22um cellulose acetate membrane to filter and dehydrate the immersion liquid, and redissolve the bacterial solution on the filter membrane with 10mL sterilized physiological saline to obtain a solution containing Phaffia rhodozyma cells. After dilution (diluted 10-1000 times), smear the plate, and the separation medium used is YM medium;

[0050] Pretreatment of surface water with Phaffia rhodozyme: dehydrate the surface water with Phaffia rhodozyme with 0.22um cellulose acetate membrane, redissolve the bacterial solution on the filter membrane with 10mL sterile saline, Appropria...

Embodiment 3

[0060] The detection of embodiment 3 astaxanthin content

[0061] First, the standard curve of astaxanthin was detected by spectrophotometer, such as Figure 4 shown.

[0062] The standard curve detection method of astaxanthin is as follows: Weigh 1.000mg of pure astaxanthin (purified astaxanthin produced by sigma company), dissolve in absolute ethanol (use 0.5mL dichloromethane to help dissolve) and dilute to 100mL , to obtain 10mg / L astaxanthin standard substance, measure astaxanthin standard substance 0mL, 0.5mL, 1mL, 2mL, 3mL, 4mL respectively, and dilute to 10mL with absolute ethanol to obtain concentrations of 0mg / L, 0.5 mg / L, 1mg / L, 2mg / L, 3mg / L, 4mg / L astaxanthin standard solutions, measure the absorbance of each standard solution at 478nm with a spectrophotometer, and the blank control is absolute ethanol. Such as Figure 4 Shown in terms of absorbance value OD 480 is the abscissa, and taking the astaxanthin content (mg / L) as the ordinate, obtains the linear relat...

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Abstract

The invention belongs to the field of biological fermentation engineering, and provides a Phaffia rhodozyma strain rich in astaxanthin and a screening method and application of the Phaffia rhodozyma strain in order to industrially produce astaxanthin through Phaffia rhodozyma. The Phaffia rhodozyma strain is named as Phaffia rhodozyma XM-1601 which is preserved in the China General Microbiological Culture Collection Center on May 3rd, 2016, and the preservation number is CGMCC No. 12413. The fermentation biomass dry weight of Phaffia rhodozyma reaches 65 g / L, the astaxanthin fermentation yield reaches 320 mg / L, and the astaxanthin content is about 4.92 mg / g. The Phaffia rhodozyma strain XM-1601 is widely applied to the fields of animal feed additives, food additives, healthcare products and the like.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a Phaffia rhodozyme strain rich in astaxanthin, a screening method and application thereof. Background technique [0002] Astaxanthin (3,3'-dihydroxy-4,4'-diketo-β,β'-carotene, Astaxanthin), also known as astaxanthin, is a kind of carotenoid with molecular formula C 40 h 52 o 4 , the molecular weight is 596.86, and its molecular structure is as follows figure 1 shown. Astaxanthin has the ability to promote antibody production, enhance host immune function, resist oxidation, and quench free radicals. It is of great significance for improving human immunity and reducing diseases. At the same time, astaxanthin also has the ability to inhibit tumor growth, improve vision and fertility. Ability and other aspects of biological functions. Therefore, astaxanthin has broad application prospects in food, feed, fish and animal husbandry, cosmetic...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02C12P23/00C12R1/645
CPCC12N1/02C12N1/16C12P23/00C12N1/145C12R2001/645
Inventor 王杰鹏郗大兴
Owner 吉林省希玛生物科技有限公司
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