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Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition

A technology of Lactobacillus reuteri and a composition is applied in the field of use for preparing blood lipid-lowering compositions, and can solve problems such as probiotics for lowering blood lipids that are rarely discussed

Active Publication Date: 2016-08-17
GENMONT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, according to the above studies, the application of probiotics in lowering blood lipids and the possible regulatory mechanisms are rarely discussed

Method used

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  • Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition
  • Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition
  • Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition

Examples

Experimental program
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Effect test

preparation example Construction

[0023] Preparation of GMNL-263 heat-killed bacteria: Lactobacillus reuteri GMNL-263 was stored in the strain preservation bank of Jingyue Biotechnology Co., Ltd. Inoculate the standard strain Lactobacillus reuteri GMNL-263 stored in the cryovial into the MRS medium, and cultivate it in an incubator at 37±1°C for 18-36 hours. After that, the cells of the fermentation broth are concentrated, and then heat-killed by high temperature. Finally, add a protective agent and freeze-dry to make Lactobacillus reuteri GMNL-2632×10 per gram 10 cells heat-killed bacteria powder.

[0024] In addition, the scope of practical application of the present invention can be further proved by the following specific examples, but it is not intended to limit the scope of the present invention in any form.

[0025] First, 6-week-old weaned male hamsters of the malet strain were purchased from the Taiwan National Experimental Animal Center (Taipei, Taiwan), and raised in plastic cages, with 8 hamsters...

Embodiment 1

[0035] Embodiment 1: Analysis of the biochemical value content of serum, liver and feces

[0036] Serum biochemical value analysis: the test items are lipid concentration in blood, including triglyceride (TG), total cholesterol (T-CHO), high-density lipoprotein cholesterol (HDL-CHO) and low-density lipoprotein cholesterol (LDL -CHO), the above-mentioned testing items were all analyzed by the National Laboratory Animal Center of Taiwan, China.

[0037] Analysis of malonaldehyde (MDA) content in blood: Use the commercially available TBARS Assay Kit (Cayman) to measure the content of malonaldehyde in serum, follow the manufacturer's instructions for the experimental operation steps, and finally analyze it with a disc spectrometer at a wavelength of 530nm The absorbance value was measured by the instrument, and the content of cytokines in the sample was calculated based on the concentration curve of the standard substance.

[0038] Lipid analysis of liver and feces: Take 0.1 g of...

Embodiment 2

[0045] Example 2: Analysis of the effect of GMNL-263 heat-killed bacteria on mRNA gene expression in liver and adipose tissue

[0046] RT-PCR method was used to detect (I) liver tissue and (II) adipose tissue mRNA gene expression, and the detected genes were:

[0047] 1. Pro-inflammatory factors: interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α), the expression of the above genes will lead to inflammation.

[0048] 2. Genes related to lipid synthesis: fatty acid synthase (fatty acid synthase, FAS), cholesterol regulatory element binding protein-1 (SREBP-1c), peroxisome proliferator-activated receptor γ ( peroxisome prolifera proliferator-activated receptorγ, PPARγ); among them, fatty acid synthase (FAS) and cholesterol regulatory element binding protein (SREBP-1c) genes are related to the synthesis of fatty acids, and peroxisome proliferator-activated receptor γ (PPARγ) It is related to the metabolism of fatty acids.

[0049] 3. Cholesterol metabolism-related gene...

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Abstract

The invention relates to a purpose of Lactobacillus reuteri GMNL-263 for preparing a hypolipidemic composition. A preservation number of the Lactobacillus reuteri GMNL-263 is BCRC 910452 and CCTCC M 209263, and the Lactobacillus reuteri can specifically inhibit the expression of related genes for synthesis of inflammation factors and lipid as well as can increase the expression of cholesterol metabolism-related genes; and thereby, the Lactobacillus reuteri GMNL-263 can be used for preparing the hypolipidemic composition, and reaches the purpose of treatment of hyperlipidemia.

Description

technical field [0001] The present invention relates to the use of Lactobacillus reuteri GMNL-263 for the preparation of blood lipid-lowering composition, especially refers to the composition containing Lactobacillus reuteri which has the ability to inhibit tumor necrosis factor α (TNF-α), fatty acid synthesis Enzyme (FAS), cholesterol regulatory element binding protein (SREBP-1c) gene expression, and enhance the ability of low-density lipoprotein receptor (LDLR) and cholesterol 7α-hydroxylase (CYP7A1) gene expression, to achieve the purpose of reducing blood lipids. Background technique [0002] Due to factors such as the increased chance of eating out and the refined diet of modern people, modern people consume too many calories and accumulate fat in the body, so they are prone to hyperlipidemia. According to research, hyperlipidemia not only easily leads to fatty liver or atherosclerosis, but also is an important risk factor for hypertension, heart disease, stroke, diabet...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P3/06A23L1/30
Inventor 陈奕兴陈雅惠楼子齐沈庭鋆
Owner GENMONT BIOTECH
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