A kind of sampling device and sampling method of plant rhizosphere and non-rhizosphere soil

Abstract

The invention discloses a plant rhizosphere and non-rhizosphere soil sampling device and sampling method. The device includes a culture box body and a plurality of collecting tubes; the culture box body comprises a cylindrical tube body and a base plate; the cylindrical box body is composed of two half tubes, two silicone rubber strips and a plurality of clamping hoops; two half tubes are in butt joint, and two butt joint parts of the two half tubes are sealed by the two silicone rubber strips respectively; after butt joint, the two half tubes are clamped tightly to form the cylindrical tube body through the clamping hoops; the lower end of the cylindrical tube body is fixed on the base plate; the collecting tubes are respectively and vertically inserted into a soil medium in the cylindrical tube body and are in honeycomb-shaped distribution. The method comprises the following five steps: 1) preparing culture soil; 2) filling the culture soil and burying the collecting tubes; 3) sowing plant seeds; 4) culturing plants; and 5) classifying and collecting soil samples. The sampling device has the advantages of simple structure, and can accurately distinguish areas surrounding root systems. The method is based on the device, is simple and easy to implement, and has accurate sampling and high sampling efficiency.

Description

technical field [0001] The invention relates to the technical field of plant rhizosphere soil sampling, in particular to a sampling device and a sampling method for plant rhizosphere and non-rhizosphere soil. Background technique [0002] The concept of rhizosphere (Rhizosphere) was first proposed by Lorenz Hiltner in 1904, and the soil mass around the root system that is several millimeters away and affected by root growth is defined as the rhizosphere. The rhizosphere is the part where plant roots and soil are in direct contact, and its physical, chemical and microbiological properties are jointly affected by plant roots and soil, and it is also the most active place for soil microorganisms. The current device for distinguishing rhizosphere and non-rhizosphere soil samples is a compartmental root box, which is generally a square or cylindrical box. The nylon nets that the roots cannot pass through are divided into different areas, and the area on one side of the crop plan...

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Problems solved by technology

In order to improve the representativeness of the sample, the rhizosphere area is usually designed to be relatively narrow. With the growth of the root system, a vertical, horizontal root surface or cylindrical root column is finally formed, which greatly limits the free growth of the root system in the horizontal and vertical directions. It is far from the "jie"-shaped root system under natural conditions in the field, and with the extension of planting time, the volume of the root system increases, and the soil bulk density in the root bag continues to increase, which greatly limits the distribution of soil nutrients to the rhizosphere. Migration, easy to cause the lack of nutrients in the later stage of plant growth

At the same time, the compartmental root box has undergone many times of alternating dry and wet during the plant cultivation process, and it is easy to form natural cracks through the soil at the nylon mesh, which seriously affects the continuity and consistency of the soil and causes the migration of nutrients and water in the soil. difficulty

In addition, the classic root shaking method artificially shakes off the non-rhizosphere soil when the soil moisture content and soil texture are suitable, and collects a small amount of soil attached to the root system as rhizosphere soil, but when the soil moisture content is too low and the soil sand content In the case of high water content, there is too little soil attached to the root system, and the sampling efficiency is low; while in soil with high water content, such as paddy soil, there is a sampling problem that the non-rhizosphere soil is difficult to shake off

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