A kind of anti-type O foot-and-mouth disease vaccine composition and its preparation and application

Abstract

The invention discloses three kinds of O-type foot-and-mouth disease virus-like particles, a vaccine composition against O-type foot-and-mouth disease and a preparation method thereof. In the virus-like particle structure provided by the invention, the epitope of foot-and-mouth disease is effectively presented on the surface of the virus-like particle, which can well stimulate B and T cells and cause an immune response; the half-life in the serum in the body is long, the stability is good, and the validity period Long-term, continuous stimulation of immune response, especially O-type FMD virus-like particles, which are CATHAY-type mutants, solve the problem of poor immunogenicity of CATHAY-type mutants, and can effectively respond to the invasion of CATHAY-type mutants; The present invention also provides an optimized virus-like particle structure, which improves the stability of the virus-like particle, not only increases the temperature stability and acidic environment stability, but also unexpectedly finds that the immune duration of the vaccine composition of the optimized virus-like particle remarkable growth.

Description

technical field [0001] The invention relates to the field of veterinary biological products. More specifically, the present invention relates to a vaccine composition against type O foot-and-mouth disease. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease that can spread quickly and over long distances. It is the most contagious disease among mammals, and the infection of artiodactyls will cause significant economic losses worldwide. Animals affected by FMD include cattle, sheep, goats and pigs. The causative agent, foot-and-mouth disease virus (FMDV), is an aphthous sore virus of the picornavirus family. The virus is divided into seven serotypes (A, O, C, Asia 1, SAT1, SAT2, and SAT3), of which the O-type foot-and-mouth disease virus is the most prevalent. Vaccine immunization is an effective measure to control the disease and protect livestock from harm. Traditional vaccines include inactivated vaccines and attenua...

AI Technical Summary

Problems solved by technology

However, there are also the following disadvantages: 1. The virus must be produced in highly sealed equipment to prevent contamination of the surrounding environment; during the production process, the virus may not be completely killed or fully attenuated, which will cause the vaccine to contain highly toxic pathogens. The disease substance, which in turn causes the disease of the immunized animals and causes the disease to spread on a large scale

2. The validity period is very short, and often need to be frozen, which increases the difficulty of promoting the use of vaccines in rural areas

3. Inactivated vaccine immunity is indistinguishable from FMD-infected animals, which limits the export of animals

4. Production depends on FMDV, so FMDV cannot be completely eliminated

[0005] In addition, the antigenicity of each subtype of O-type FMD is different, and they cannot be mutually immune to each other. In my country, O-type FMD CATHAY and its variant strains have been prevalent for a long time, and the prevalence of SEA type in recent years has made O-type FMD Epidemics are becoming more complicated, and new vaccines that can simultaneously prevent and treat three subtypes of infection are urgently needed

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