A method for improving salt tolerance and waterlogging tolerance of cut chrysanthemum by transfecting cmsos1 gene

Abstract

The invention belongs to the field of genetic engineering technologies and transgenetic breeding and discloses a method for improving salt tolerance and flooding tolerance of Chrysanthemum morifolium by genetically modifying CmSOS1 genes. According to the method, a plant expression carrier containing the CmSOS1 genes is transferred into Chrysanthemum morifolium for overexpression, so that the salt tolerance and the flooding tolerance of Chrysanthemum morifolium are improved. The method comprises the following steps: (1), the plant expression carrier containing the CmSOS1 genes and used for overexpression is established; (2), Chrysanthemum morifolium is converted with a leaf-disc method adopting mediation with agrobacterium tumefaciens EHA105; (3), the salt tolerance and the flooding tolerance of a plant with genetically modified CmSOS1 genes are analyzed. PCR (polymerase chain reaction) detection and quantitative RT(reverse transcriptase)-PCR detection performed on resistant rooting plants verifies that CmSOS1 genes have been integrated into a genome of a transgenetic plant and subjected to transcriptional expression, salting and flooding treatment on the transgenetic plant indicates that the salt tolerance and the flooding tolerance of the overexpressed plant are improved, a novel and practical method is provided for breeding of stress-resistant varieties of chrysanthemums with a genetic engineering technology, and the breeding process of a chrysanthemum biotechnology is effectively promoted.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering technology and transgenic breeding, and relates to a method for improving the salt tolerance and waterlogging resistance of cut chrysanthemum through transgenic CmSOS1 gene, and specifically relates to the construction of plant expression vectors including CmSOS1 gene, transformed cells, and transgenic cut flowers with CmSOS1 gene Chrysanthemum cultivation, identification methods and application of transgenic plants. Background technique [0002] Salt stress and waterlogging stress are important environmental factors affecting plant growth and development among abiotic stresses. Chrysanthemum is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It has a wide range of uses and cultivation areas, has high ornamental and economic value, and occupies a very important position in modern flower production. However, in the annual cult...

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Problems solved by technology

Under normal growth conditions, SOS1mRNA is unstable, and its half-life of rapid degradation is about 10min. Reactive oxygen mediates the increase of the stability of SOS1mRNA under salt stress (Chung et al., 2008); under salt or oxidative stress, SOS1 can pass through its C-terminal The cytoplasmic long hydrophilic tail interacts with RCD1, an important signal regulator in the response to oxidative stress (Katiyar-Agarwal et al., 2006), indicating that the SOS1 gene is involved in the response of plants to oxidative stress. Regulation of water stress, but there is no related report in the field of plants

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