Hypsizigus marmoreus strain, and culture method, planting method and application thereof
A technology of mushrooms and strains, applied in the field of cultivation, Finc-B-5 strains of mushrooms and their cultivation, can solve the problems of long cultivation period, long production cycle and unsatisfactory taste of mushrooms, and achieve the benefits of The effect of long-distance transportation, high yield per unit area, and high consumer acceptance
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Embodiment 1
[0050] Embodiment 1 hybridization obtains Finc-B-5
[0051] The strain Finc-B-5 was obtained by conventional cross-breeding method.
[0052] Specific steps are as follows:
[0053]1) Select parent strains: select bacterial strain TNN-11, bacterial strain H-W and bacterial strain Bodo A-264 as parents, and the fruiting bodies of these three parents can be purchased in the market;
[0054] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;
[0055] 3) 600 heterozygotes were obtained by conventional cross-breeding methods;
[0056] 4) Cultivation and screening: The obtained heterozygotes are subjected to cultivation tests according to conventional methods, and through systematic screening, a target bacterial strain is obtained from 600 heterozygotes, and the bacterial strain is named Finc-B-5. Its cultivation and The cultivation cycle is short, ...
Embodiment 2
[0057] Embodiment 2 Antagonism experiment
[0058] Take the mushroom strain Finc-B-5, strain TNN-11, strain H-W, strain Bodo A-264 and strain TNN-12 of the present invention as test materials, and the medium used is PDA medium.
[0059] Strain Finc-B-5, strain TNN-11, strain H-W, strain Bodo A-264, and strain TNN-12 were inoculated and cultured on a flat medium, respectively, with a distance of 3 cm between the inoculation points and cultured at a constant temperature of 23°C for 31 days. See Table 1 for the results.
[0060] Table 1 Antagonism test among different strains of jijiji mushrooms
[0061] Variety
TNN-11
H-W
Bodo A-264
TNN-12
Finc-B-5
+
+
+
+
[0062] Note: "+" indicates antagonism.
[0063] Such as Figure 1-8 As shown, on the front and back of the junction of confrontation culture colonies, strain Finc-B-5 has obvious antagonistic lines with strain TNN-11, strain H-W, strain Bodo A-264 and strain TNN-12,...
Embodiment 3
[0064] Embodiment 3 cultivation experiment
[0065] The mushroom strain Finc-B-5 of the present invention, the most widely planted bacterial strain TNN-12 among the existing mushroom varieties and the shortest cultivation cycle Finc-B-1 of the mushroom that we can obtain (disclosed in Chinese Patent In CN103004464A, the bacterial strain was preserved in the China Typical Culture Collection Center on August 13, 2012, and the preservation number is CCTCCM2012308) respectively carried out the optimization experiment of the cultivation cycle and the cultivation cycle, and each bacterial strain cultivated 80 bottles each time. The yield per unit area is compared, and the advantages and characteristics of the mushroom strain Finc-B-5 of the present invention are summarized.
[0066] The cultivation conditions that present embodiment adopts are as follows:
[0067] See cultivar culture medium for culture medium, no more details.
[0068] Cultivation bottle volume: 850cc.
[0069] ...
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