Bacillus subtilis fertilizer and preparation method and application thereof
A technology of Bacillus subtilis and bacterial fertilizer, applied in the field of agricultural microorganisms, can solve the problems of reduced quality of potato pieces, infection, tuber rot, etc., and achieve the effects of improving yield and quality, non-toxic side effects, and robust growth
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Embodiment 1
[0043] Embodiment 1: Acquisition, identification and preservation of Bacillus subtilis JQ-001 strain
[0044] 1. Obtaining of Bacillus subtilis JQ-001 strain:
[0045] The Bacillus subtilis of the present invention is obtained from the rhizosphere soil of Jiansanjiang rice in Heilongjiang Agricultural Reclamation by adopting the soil dilution separation method to separate and obtain, and the separation method is:
[0046] 1. Soil sample collection: During the normal growth period of rice, dig out the rice roots, take the soil on the roots, take several samples from one place, mix them with coarse sieves, and take about 200g for separation;
[0047] 2. Culture medium plate preparation: Melt NA medium and LB medium, pour them into sterilized petri dishes respectively, and make NA and LB plates after cooling and solidifying;
[0048] 3. Weigh 1g of soil sample into a 250ml Erlenmeyer flask with glass beads and 100ml of sterilized water, and vibrate evenly on a shaker to make 10 ...
Embodiment 2
[0059] Embodiment 2: Determination of the antagonistic effect of JQ-001 bacterial strain
[0060] Solid medium: NA medium, Gao's No. 1 medium: soluble starch 20g, KNO 3 1g, NaCl0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g, FeSO 4 0.01g agar powder 16g, distilled water 1000ml, pH7.4, when preparing, first use a small amount of cold water to make starch into a paste, pour into boiling water, heat on the fire, add other ingredients while stirring, after melting, make up When the water content reaches 1000ml, then use 100ml Erlenmeyer flasks to evenly distribute into 13 bottles, and sterilize at 121°C for 30 minutes.
[0061] Liquid medium (subtilis fermentation medium): glucose 20g, corn steep liquor powder 5g, peptone 1g, yeast powder 0.5g, MnSO 4 20ml, MgSO 4 10ml, 500ml tap water, pH7.5, sterilized at 121°C for 30min.
[0062] 1. Experimental method: plate confrontation culture method
[0063] 1. Isolation, purification and cultivation of potato scab pathogen
[0064] Sampling l...
Embodiment 3
[0076] Embodiment 3: Preparation and application of Bacillus subtilis JQ-001 microbial fertilizer
[0077] 1. Preparation of bacterial fertilizer
[0078] 1. Activation of Bacillus subtilis strains
[0079] Inoculate the strains stored in the -20°C refrigerator into NA medium test tubes, and incubate at a constant temperature of 37°C for 24 hours;
[0080] 2. Preparation of primary seeds
[0081] Prepare an appropriate amount of NA medium, divide it into two eggplant-shaped bottles, and after the high-pressure sterilization is completed, take it out and tilt it to make a slant medium, insert the activated Bacillus subtilis JQ-001, and cultivate it in a 37°C constant temperature incubator 2d;
[0082] 3. Preparation of secondary seeds
[0083] The strains cultivated in eggplant-shaped bottles are then transferred to seed tanks containing fermentation medium for cultivation. The cultivation conditions are temperature 37°C and ventilation volume 30m 3 / h, the rotation speed ...
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