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Stem cell preparation for cornea repair and preparation method and application thereof

A stem cell preparation and stem cell technology, applied in the biological field, can solve problems such as poor repair efficiency and poor operability, and achieve the effects of improving repair ability, promoting healing, and stimulating cell proliferation

Inactive Publication Date: 2016-07-27
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a corneal repairing stem cell preparation and its Preparation method and application

Method used

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  • Stem cell preparation for cornea repair and preparation method and application thereof
  • Stem cell preparation for cornea repair and preparation method and application thereof

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preparation example Construction

[0025] Further, the preparation method of the stem cell preparation of the present invention comprises the following steps:

[0026] S1. Obtaining mesenchymal stem cells;

[0027] S2, extract polysaccharide;

[0028] S3. Mixing and preparing a stem cell preparation.

[0029] In step S1, the present invention preferentially uses bone marrow mesenchymal stem cells of any generation from the 3rd to the 5th generation, and the specific acquisition process is as follows:

[0030] S11. Screening mesenchymal stem cells by density gradient centrifugation combined with adherent culture;

[0031] Extract the bone marrow, fully mix with the basal medium, filter, add Percoll solution, centrifuge, collect the buffy coat layer, which is the mononuclear cells, wash, and then culture with the basal medium containing fetal bovine serum, penicillin and streptomycin, After 24 to 72 hours, the medium was replaced to remove non-adherent cells, and the medium was changed every 2 to 4 days until ...

Embodiment 1

[0046] S1. Obtaining mesenchymal stem cells;

[0047] S11. Screening mesenchymal stem cells by density gradient centrifugation combined with adherent culture;

[0048] Immediately inject the extracted bone marrow into a centrifuge tube containing 5ml of low-sugar DMEM and mix thoroughly. After filtering through a 200-mesh stainless steel filter, gently add the suspension to the preset equal volume of Percoll solution (density 1.082kg / L, Purchased from GE Company, the U.S., Percoll is a suspension of silica gel particles processed through polyvinylpyrolidone (PVP), which is non-toxic and irritating to cells), centrifuged at 2400r / min for 20min, and collected cloudy white film Layer mononuclear cells, wash twice with PBS (1200r / min, 10min), resuspend the cells with low-sugar DMEM medium containing 100mL / L fetal bovine serum, 100U / mL penicillin, 100u / mL streptomycin, and adjust the cell density Take 5×10 5 / mL was inoculated in a 50mL culture flask and placed in CO 2 Incubate ...

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Abstract

The invention relates to a stem cell preparation for cornea repair.The stem cell preparation comprises polysaccharide, KGF-2 and mesenchymal stem cells.By combining the polysaccharide, the KGF-2 and the mesenchymal stem cells, the anti-inflammatory effect is achieved, cornea surface cell proliferation can be promoted, cornea epithelial cell proliferation is stimulated, cornea epithelium repair ability and cornea transparency are improved, cornea excessive fibrosis can be prevented, and therefore healing of a damaged cornea is promoted.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a stem cell preparation for repairing cornea and its preparation method and application. Background technique [0002] Corneal chemical injury is one of the most common diseases in emergency department of ophthalmology, and it is also a relatively common blinding eye disease. Corneal chemical injury accounts for 8% of all eye injuries in foreign countries, while in China it accounts for 15% of eye injuries, and the incidence rate is much higher than that in developed countries such as Europe and America. At present, many studies have reported various methods for the treatment of corneal alkali burns, including drug treatment such as antibiotics, steroid hormones, ascorbic acid, collagenase inhibitors, etc., and surgical treatment such as corneal transplantation and amniotic membrane transplantation. the purpose of treatment. Nevertheless, these treatments do not adequately address ...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P27/02C08B37/00A61K35/28A61K31/715A61K31/727A61K31/7036A61K31/43
CPCA61K38/18A61K31/43A61K31/7036A61K31/715A61K31/727A61K35/28A61K36/898C08B37/00A61K2300/00
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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