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Bacillus subtilis and application thereof

A technology of Bacillus subtilis and strains, applied in the field of ecology, can solve problems such as deficiencies, and achieve the effects of low cost, good control effect, and environmental safety

Active Publication Date: 2016-07-20
QINGHAI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of Bacillus subtilis isolated and purified from highland barley distiller's grains and its biocontrol application. The technical problem aimed at solving is to address the deficiencies in the existing spring wheat field and highland barley field oat weed weed control technology

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Experimental materials and methods

[0023] 1.1 Isolation and purification of strains and preparation of fermentation broth

[0024] In the highland barley wine cellar, press the top, middle and bottom to retrieve the fresh highland barley distiller's grains, take 5g of highland barley distiller's grains samples respectively, put them in 45mL sterile water, shake at room temperature for 30min, pipette 1mL and add 9mL sterile water to prepare 10 -1 bacterial suspension. And so on, dilute step by step 10 -2 、10 -3 . Take 0.2 mL of the bacterial suspension and spread it on the beef extract peptone medium, and place it in a 30°C incubator for cultivation. After 3 days of cultivation, take mycelium pieces from the edge of the colony and inoculate them into a culture bottle with 200mL NYD culture solution, 5 pieces per bottle, 30°C, 180r / min -1 After culturing for 5 days, the culture solution was vacuum filtered, and the filtrate was filtered through a 0.45 μm microp...

Embodiment 2

[0062] According to the method described in Example 1, the fermentation filtrate was prepared, and the outdoor preliminary test of Bacillus subtilis JZ2-4-5 controlling spring wheat and highland barley field weeds was tested. The outdoor experiment was carried out in the experimental field of the Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences. The weeds are mainly wild oats (2-4 leaf stage), and the area of ​​the plot is about 4m 2 , spraying amount 200ml, repeated 3 times, 20 days and 40 days after spraying, the control effect and fresh weight effect of wild oats were investigated. The results are shown in Table 3.

[0063] Table 3 Control effect of Bacillus subtilis JZ2-4-5 on spring wheat and highland barley field oat

[0064]

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Abstract

The invention discloses a strain of bacillus subtilis obtained from fermented grains of highland barley liquor through separation and purification and biocontrol application of the bacillus subtilis. A classification name of the strain is bacillus subtilis, a complete name is bacillus subtilis JZ2-4-5, and the strain is collected in the China General Microbiological Culture Collection Center in 3#, Yard 1, West Beichen Road, Chaoyang District, Beijing, China with the collection number CGMCC No.12219 on March 16, 2015. The bacillus subtilis JZ2-4-5 is used for controlling grassy weeds, namely avena fatua, in spring wheat and highland barley fields, and has good biocontrol effects on avena fatua plants in a 2-leaf 1-top shoot period and a 4-leaf 1-top shoot period, and avena fatua. seed germination and seedling growth can be effectively inhibited.

Description

technical field [0001] The invention belongs to the field of ecology, and in particular relates to a bacillus subtilis strain derived from highland barley distiller's grains and its biocontrol application. Background technique [0002] Weeds in farmland directly or indirectly affect agricultural production, affecting the yield, quality and quality of crops. At present, chemical herbicides are still the mainstay of farmland weed control. With the widespread use of chemical herbicides, the negative effects are becoming more and more obvious. my country has put forward the goal of "double reduction (reduction of chemical fertilizers and pesticides)" in the "13th Five-Year Plan" of agriculture. Herbicides account for more than 50% of the total pesticide market. Therefore, in order to successfully achieve this goal, we must find ways to significantly reduce the amount of chemical herbicides used. The development of biological herbicides is an important basis for replacing and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/02A01P13/02C12R1/125
CPCA01N63/10C12N1/02C12N1/20C12N1/205C12R2001/125
Inventor 李玮沈硕郭青云
Owner QINGHAI UNIVERSITY
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