Sea cucumber compound feed
A compound feed and sea cucumber technology, applied in the field of sea cucumber breeding, can solve the problems of affecting the survival and growth of sea cucumbers, increasing the cost of breeding, restricting the use of soybean meal, etc., and achieve the effects of promoting the growth of sea cucumbers, reducing the bait coefficient, and reducing the cost of feed
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[0019] Specific embodiments of the present invention will be described below.
[0020] 1. Preparation of Aspergillus niger spore suspension:
[0021] Inoculate the preserved and rejuvenated Aspergillus niger fungus strains on the PDA medium by punching holes, and cultivate them at 28°C for 3 days. After the hyphae cover the entire plate, rinse the spores on the medium with sterile water A suspension of Aspergillus niger spores (i.e. fungal seed liquid) was obtained.
[0022] Koji fermentation:
[0023] Take a certain amount of soybean meal, add water according to the ratio of material to water 80%, mix well, autoclave at 121°C for 20min, and cool to room temperature. Put it into a fermentation container (sterile), insert the Aspergillus niger spore suspension (10% v / w inoculum), put it in a 31°C incubator, and cultivate it for 2 days. During the cultivation process, stir once every 24 hours and add sterile water (replenish the lost water according to the water content to ma...
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