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Radix bupleuri virus free tissue culture rapid propagation method

A technology of tissue culture rapid propagation and Bupleurum, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of pollution, low survival rate, can not completely remove viruses, etc. The effect of simple poisoning and improved survival rate

Inactive Publication Date: 2016-07-20
岳兰兰
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem that this shoot tip meristem detoxification method exists is: to cut very small shoot tip meristem, need to operate under microscope, has certain difficulty; The cut shoot tip tissue is too large, and the virus cannot be completely removed, and it is easy to cause pollution; therefore, a better method of virus removal is still needed

Method used

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Embodiment l

[0029] 1) Selection and sterilization of explants: Select new seeds harvested in the same year that grow vigorously, have no pests and diseases, and have full grains, put the seeds in water at 55°C and keep stirring until room temperature, and remove the seeds floating on it. Then wash the plump grains that sink to the bottom, soak in 75% alcohol for 0.5-1.0min, then sterilize with 0.1% mercuric chloride solution for 20min, and finally rinse with sterile water for 3-5 times, and use it as a foreign tissue for detoxification tissue culture. Implant;

[0030] 2) Basic medium: select MS basic medium, sucrose 25g / L, agar 8g / L, peptone 3-8g / L, humic acid 0.5g / L, pH6.2;

[0031] 3) Induction culture: under sterile conditions, inoculate the seeds in the induction medium of MS+6-BA0.5mg / L+α-sodium naphthalene acetate 0.1mg / L+NAA0.15mg / L+vitamin B20.001mg / L Above, the callus is formed first, and then the callus is transplanted to the differentiation medium of MS+6-BA2.5mg / L+NAA0.2mg / L...

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Abstract

The invention discloses a radix bupleuri virus free tissue culture rapid propagation method. The method comprises the following steps: choosing strong and plump seeds, which are harvested in the current year and do not have any disease or pest; soaking seeds in water with a temperature of 55 DEG C, continuously stirring the water until the temperature of the water reaches the room temperature; removing the floating empty seeds, cleaning the plump seeds in the bottom, soaking the seeds by alcohol (75%) for 0.5 to 1.0 minute, then sterilizing the seeds for 20 to 30 minutes by mercury dichloride, finally washing the seeds by sterile water for 3 to 5 times, taking the seeds as explants; carrying out induction culture, proliferation culture, rooting culture, transplanting, and virus detection, and largely breeding virus free tissue culture seedlings and virus free plants. Compared with the conventional radix bupleuri breeding method, the provided method has the advantages of low pollution rate of inoculation, high survival rate of transplanting, and high quality of radix bupleuri.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation of Chinese herbal medicine, and in particular relates to a method for tissue culture and rapid propagation of Bupleurum bupleurum. Background technique [0002] Bupleurum is a perennial herb with a plant height of 40-85cm, compound umbels, yellow flowers, irregularly branched roots, varying in thickness, taproot is often not obvious, and the skin is black or white. Stems and leaves grow mainly, and roots grow later. The rhizome of Bupleurum bupleurum is used as medicine, which has the effects of soothing the liver, relieving heat, and relieving pain. It is mainly used to treat colds, fever, chronic hepatitis, rib pain or intercostal neuralgia, and gynecological diseases. According to related reports, the annual demand for Bupleurum bupleurum is more than 3 million kilograms, which has the characteristics of high medicinal value, promising development prospects, and st...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 岳兰兰
Owner 岳兰兰
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