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Method for utilizing test tube potatoes for efficiently producing detoxication breeder's seeds

A test-tube potato and original seed technology, which is applied in the field of efficient production of virus-free original seed, can solve the problems affecting the healthy and rapid development of the virus-free original seed industry, increasing the labor cost of the virus-free original seed, and re-infection of bacteria. , to achieve the effect of promoting the production scale of detoxified original seeds, easy mechanized planting, and storage resistance

Inactive Publication Date: 2016-07-20
李文超 +2
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AI Technical Summary

Problems solved by technology

[0002] At present, the production of the virus-free original seed of potato is carried out under artificial isolation and insect-proof conditions and good cultivation conditions by using the tissue-cultured seedlings that have been stripped and detoxified from the shoot tips cultivated in the tissue culture room; The virus-free original seed has obvious growth advantages, which can not only increase the yield, but also improve economic benefits. It is an effective way to solve the degradation of potatoes; however, the production of virus-free tissue culture seedlings requires a lot of labor and has seasonal limitations. Propagate to a large enough scale during the planting season; the tissue culture seedlings are relatively weak, the survival rate is low, and it is easy to attract the re-infection of pathogens after cultivation; the tissue culture seedlings are not resistant to storage and long-distance transportation, and can only be carried out at the production site Propagation of detoxified original seedlings; planting of tissue cultured seedlings can only be carried out manually, and cannot be mechanized at all, and the work efficiency is low, which increases the labor cost of the production of detoxified original seedlings; due to the above-mentioned problems of tissue cultured seedlings, it directly affects The healthy and rapid development of the virus-free original seed industry, therefore, it is urgent to improve the cultivation method of the virus-free original seed of potato to meet the planting needs

Method used

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Embodiment 1

[0027] A method for efficiently producing the virus-free original seed by using test tube tubers of potato, comprising the following steps:

[0028] 1) Preparing for the warm net room: install insect-proof nets, plastic shed films and sunshade nets, and insect-proof nets of 60 mesh to ensure tightness and no loopholes;

[0029] 2) Germination of test-tube tubers: before sowing, test-tube tubers that break dormancy should be placed at 20-25°C in room temperature and dark conditions for 15-20 days of germination, and the concentration of tuber tubers that have not broken dormancy is 15 mg L -1 Soak in the gibberellin solution for 5-10 minutes, take it out and put it in a tray, cover it with damp gauze, keep it moist every day, put it at room temperature of 20-25°C, and germinate for 10-15d;

[0030] 3) Substrate preparation: put the fungus residue into a plastic turnover basket and autoclave for 30 minutes; then mix the vermiculite and fungus residue with a volume ratio of 1:2 t...

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Abstract

The invention discloses a method for utilizing test tube potatoes for efficiently producing detoxication breeder's seeds.The method comprises the following steps of green net house preparation, test tube potato germination acceleration, matrix preparation, cultivation frame preparation, environmental disinfection, sowing, management after planting and breeder's seed harvesting and storing, wherein the test tube potatoes whose dormancy is not broken are soaked with a gibberellin solution for germination acceleration; a matrix is formed by mixing vermiculite and mushroom dreg, and potassium sulfate compound fertilizer is applied to the matrix; sowing is carried out according to the amount of 250 particles / m<2>, the planting distance is 5 cm, and the line distance is 8 cm; management after planting comprises temperature management, water management, matrix cultivation, fertilization management and disease and pest preventing management; after being harvested, the breeder's seeds are sorted and packaged into detoxification seed potato net bags and stored at the temperature ranging from 2 DEG C to 4 DEG C.The method has the advantages of being high in yield, high in economic benefit, storable and convenient to transport, secondary infection of pathogenic bacteria is eradicated, the potatoes can be planted mechanically, convenience is brought to cultivation and management, the survival rate is high, and the method has great significance in demonstration and popularization of production scales of detoxication breeder's seeds.

Description

technical field [0001] The invention relates to the technical field of potato cultivation, in particular to a method for efficiently producing virus-free original seeds by using test tube tubers of potato. Background technique [0002] At present, the production of the virus-free original seed of potato is carried out under artificial isolation and insect-proof conditions and good cultivation conditions by using the tissue-cultured seedlings that have been stripped and detoxified from the shoot tips cultivated in the tissue culture room; The virus-free original seed has obvious growth advantages, which can not only increase the yield, but also improve economic benefits. It is an effective way to solve the degradation of potatoes; however, the production of virus-free tissue culture seedlings requires a lot of labor and has seasonal limitations. Propagate to a large enough scale during the planting season; the tissue culture seedlings are relatively weak, the survival rate is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G31/06
CPCA01G31/00A01G31/06Y02P60/21
Inventor 李文超陈理清唐艳梅
Owner 李文超
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