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A method of measuring fo to estimate the primary productivity of a water body

A technology of primary productivity and water body, applied in the direction of testing water, material inspection products, etc.

Inactive Publication Date: 2018-02-23
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature or patent information to describe the use of modulating the chlorophyll fluorescence parameter Fo to characterize the content of chlorophyll a or the density of microalgae cells

Method used

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  • A method of measuring fo to estimate the primary productivity of a water body
  • A method of measuring fo to estimate the primary productivity of a water body
  • A method of measuring fo to estimate the primary productivity of a water body

Examples

Experimental program
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Effect test

Embodiment 1-3

[0034] Examples 1-3 Measuring the chlorophyll a content of pure Chromococcus, Acetococcus and Chlorella culture fluids by means of Fo.

[0035] Dilute the culture solution of Chromococcus, Acylella and Chlorella (algae species are all from the algae species bank of Institute of Hydrobiology, Chinese Academy of Sciences) cultured to the logarithmic phase in 2-fold gradients 5 times to obtain 6 concentration gradients.

[0036] Take 1 mL of each concentration of algae solution into a centrifuge tube, centrifuge at 12,000 rpm for 3 min at room temperature, discard the supernatant, add 1 mL of methanol to the pellet, shake and resuspend, and store at 4°C in the dark for more than 12 h. Then, centrifuge at 12000rpm for 5 minutes, transfer the supernatant to a cuvette, and measure the absorbance OD at 652.4nm and 665.2nm using a spectrophotometer (Shanghai Meipuda Instrument Co., Ltd.) 652.4 and OD 665.2 (Methanol is the blank control). Chlorophyll a content was calculated by the ...

Embodiment 4-5

[0043] Embodiment 4-5 Chlorophyll of aquaculture water body

[0044] The water sample of aquaculture used in the present invention comes from an aquaculture pond in Sheyang County, Yancheng City, Jiangsu Province. After testing, the water sample of the water body mainly contains the following photosynthetic microalgae: Cystis, Microcystis pisces, Anabaena, Pseudo Anabaena; Diatoms such as Cyclotella, Stratella, Coniferium, Navicula; Green algae, such as Chlamydomonas, Cystoma, Chlorella , Scenedesmus, Panstar algae; cryptophyta; euglena; dinoflagellates. On June 5, 2015, the water sample was collected in the pond, 300ml, and the sampling depth was 1m, concentrated to 6ml by suction filtration (Jinteng diaphragm vacuum pump, filter membrane aperture 0.45 μm), and established Fo- Chlorophyll a content correlation curve ( Figure 4 ), the correlation equation obtained is y=0.00008754x-0.31527133, R 2 =0.97082079, where x and y are Fo and chlorophyll a content (mg L -1 ).

[...

Embodiment 6-8

[0051] Examples 6-8 Measuring the cell density of the pure Plasmodium, Chlamydomonas, and Chlamydomonas culture fluids by means of Fo.

[0052] The culture solution of Planischidella, Cyclotella, and Chlamydomonas (the algae species are all from the algae species library of the Institute of Hydrobiology, Chinese Academy of Sciences) cultivated to the logarithmic phase was diluted 5 times with a 2-fold gradient to obtain 6 concentration gradients. Take 1 mL of each concentration of algae liquid for cell counting. The counting method is:

[0053] Clean the counting plate (XB-K-25, Shanghai Qiujing Biochemical Reagent Instrument Co., Ltd.) until there is no dirt in the microscope, dry it with a hair dryer, and cover with a cover glass; blow the algae solution with a pipette to make it evenly dispersed. Then drop it on the edge of the cover glass, let the algae solution automatically enter the counting chamber along the gap by capillary osmosis, and there should be no air bubbles...

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Abstract

The invention provides a method for measuring Fo to represent chlorophyll a content. The method comprises the following steps: establishing an Fo-chlorophyll a content coherency equation; measuring the Fo of a sample; substituting the Fo into the obtained equation, and calculating the chlorophyll a content. The invention further provides a method for estimating primary productivity of a water body, and the method comprises the step of substituting the chlorophyll a content obtained in the previous method into an equation of P=K.r.c (Chla).DH so as to obtain the primary productivity of the water body. The method further provides a method for measuring algae cell density, and the method comprises the following steps: establishing an Fo-algae cell density coherency equation; measuring the Fo of a sample; substituting the Fo into the obtained equation, and calculating the algae cell density. By adopting all the methods, chlorophyll content and algae cell density in a photosynthetic microalgae culture solution, an aquaculture water body or a natural water body can be easily measured, the primary productivity in the aquaculture water body or the natural water body can be easily estimated, the health level of the water body is assessed, and further a necessary basis is provided for aquaculture, environment monitoring and the like.

Description

technical field [0001] The present invention relates to the field of microalgae, and more particularly, the present invention relates to the use of Fo to measure the chlorophyll a content and algal cell density in a water body containing microalgae, and thereby estimate the primary productivity of the water body. Background technique [0002] Primary (or primitive) productivity, also known as water primary productivity, is the ability of photosynthetic plants (including bacteria) in water to fix the energy of sunlight to produce organic matter per unit area and unit time. Primary productivity is one of the important evaluation bases in the fields of aquaculture feeding amount and water environment nutrient type. In order to estimate the primary productivity, it is often necessary to measure the content of chlorophyll a, and then use the formula P=K r c(Chla) DH, where: P is the primary productivity, mg / (m 3 d); r is the assimilation coefficient; c(Chla) is the content of ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18
CPCG01N33/18
Inventor 王强吴宗文谢伟陈辉何晨柳陈为先
Owner INST OF AQUATIC LIFE ACAD SINICA
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