A method of measuring fo to estimate the primary productivity of a water body
A technology of primary productivity and water body, applied in the direction of testing water, material inspection products, etc.
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Embodiment 1-3
[0034] Examples 1-3 Measuring the chlorophyll a content of pure Chromococcus, Acetococcus and Chlorella culture fluids by means of Fo.
[0035] Dilute the culture solution of Chromococcus, Acylella and Chlorella (algae species are all from the algae species bank of Institute of Hydrobiology, Chinese Academy of Sciences) cultured to the logarithmic phase in 2-fold gradients 5 times to obtain 6 concentration gradients.
[0036] Take 1 mL of each concentration of algae solution into a centrifuge tube, centrifuge at 12,000 rpm for 3 min at room temperature, discard the supernatant, add 1 mL of methanol to the pellet, shake and resuspend, and store at 4°C in the dark for more than 12 h. Then, centrifuge at 12000rpm for 5 minutes, transfer the supernatant to a cuvette, and measure the absorbance OD at 652.4nm and 665.2nm using a spectrophotometer (Shanghai Meipuda Instrument Co., Ltd.) 652.4 and OD 665.2 (Methanol is the blank control). Chlorophyll a content was calculated by the ...
Embodiment 4-5
[0043] Embodiment 4-5 Chlorophyll of aquaculture water body
[0044] The water sample of aquaculture used in the present invention comes from an aquaculture pond in Sheyang County, Yancheng City, Jiangsu Province. After testing, the water sample of the water body mainly contains the following photosynthetic microalgae: Cystis, Microcystis pisces, Anabaena, Pseudo Anabaena; Diatoms such as Cyclotella, Stratella, Coniferium, Navicula; Green algae, such as Chlamydomonas, Cystoma, Chlorella , Scenedesmus, Panstar algae; cryptophyta; euglena; dinoflagellates. On June 5, 2015, the water sample was collected in the pond, 300ml, and the sampling depth was 1m, concentrated to 6ml by suction filtration (Jinteng diaphragm vacuum pump, filter membrane aperture 0.45 μm), and established Fo- Chlorophyll a content correlation curve ( Figure 4 ), the correlation equation obtained is y=0.00008754x-0.31527133, R 2 =0.97082079, where x and y are Fo and chlorophyll a content (mg L -1 ).
[...
Embodiment 6-8
[0051] Examples 6-8 Measuring the cell density of the pure Plasmodium, Chlamydomonas, and Chlamydomonas culture fluids by means of Fo.
[0052] The culture solution of Planischidella, Cyclotella, and Chlamydomonas (the algae species are all from the algae species library of the Institute of Hydrobiology, Chinese Academy of Sciences) cultivated to the logarithmic phase was diluted 5 times with a 2-fold gradient to obtain 6 concentration gradients. Take 1 mL of each concentration of algae liquid for cell counting. The counting method is:
[0053] Clean the counting plate (XB-K-25, Shanghai Qiujing Biochemical Reagent Instrument Co., Ltd.) until there is no dirt in the microscope, dry it with a hair dryer, and cover with a cover glass; blow the algae solution with a pipette to make it evenly dispersed. Then drop it on the edge of the cover glass, let the algae solution automatically enter the counting chamber along the gap by capillary osmosis, and there should be no air bubbles...
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