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Kit for predicting myopia risk of child and application thereof

A technology of myopia and kit, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., which can solve the problems of unpredictable risk and difficulty in evaluating the results of myopia risk, and achieve low false positive and false negative rates, The effect of high accuracy and low equipment requirements

Inactive Publication Date: 2016-07-06
TIANJIN MARVEL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The problem of the prior art that the present invention needs to solve is: the existing myopia risk prediction products only aim at genetic factors, cannot predict the risk of exposure to the environment, and it is difficult to evaluate the result of myopia risk caused by the joint action of genetic factors and environmental factors

Method used

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  • Kit for predicting myopia risk of child and application thereof
  • Kit for predicting myopia risk of child and application thereof
  • Kit for predicting myopia risk of child and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 provides a kit for predicting the risk of myopia in children. The invention includes a first reagent for detecting the mutation site rs10034228 of the MYP11 gene and a second reagent for detecting the mutation site rs188663068 of the APLP2 gene. The first reagent includes a reagent for MYP11 gene amplification and a reagent for MYP11 gene restriction detection, and the second reagent includes a reagent for APLP2 gene amplification and a reagent for APLP2 gene restriction detection.

[0062] The reagents for MYP11 gene amplification include: primer 1 and primer 2, the sequence of primer 1 is SEQ ID No.1, and the sequence of primer 2 is SEQ ID No.2;

[0063] The reagents for APLP2 gene amplification include: primer 3 and primer 4, the sequence of the primer 3 is SEQ ID No.3, and the sequence of the primer 4 is SEQ ID No.4.

[0064] The reagents used for MYP11 gene digestion detection include restriction endonuclease RasI;

[0065] The reagents used for dete...

Embodiment 2

[0098] The difference between the second embodiment and the first embodiment is that

[0099] The PCR amplification products were subjected to restriction endonuclease digestion, and the reaction was prepared according to the restriction endonuclease digestion system in Table 3 and Table 4, and reacted in a 37°C incubator for 2 hours, followed by electrophoresis.

[0100] Select another 6 samples respectively, the source of the samples is kindergarten children aged 3-5, and name them as sample 7 to sample 12 respectively. Wherein, the electrophoresis result of embodiment two is as follows image 3 and Figure 4 shown. in, image 3 It is the electropherogram of the MYP11 gene fragment digested with RsaI. Among them, lanes 1 to 7 are respectively from left to right, lanes 1-6 are test samples, and lane 7 is DNAMarker. According to the interpretation of the electrophoresis results in Table 5, there is only one band in the sixth lane, and the corresponding sample genotype is TT...

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Abstract

The invention relates to the field of gene detection engineering, in particular to a kit for predicting the myopia risk of a child and application thereof.The kit includes a first reagent for detecting a mutation site rs10034228 of an MYP11 gene and a second reagent for detecting a mutation site rs188663068 of an APLP2 gene.The kit reflects the myopia risk brought by two factors of inheritance and environment through polymorphism detection on the APLP2 gene and the MYP11 gene.In addition, a restriction endonuclease method is adopted to detect the polymorphism of SNP sites of the gene MYP11 and APLP2, the accuracy is high, the specificity is good, false positive and false negative rates are low, the operation is simple and convenient, the requirements for instruments and devices are low, and the adaptability is good.

Description

technical field [0001] The invention relates to the field of gene detection engineering, in particular to a kit for predicting the risk of myopia in children and an application thereof. Background technique [0002] Myopia refers to the optical state in which parallel light rays (5 meters away) pass through the refractive system of the eyeball and form a focal point in front of the retina when the eye is in a relaxed state. Myopia, also known as short-sightedness, is an eye that can only see near but not far. After being refracted by the refractive system of the eye, the parallel light from infinity gathers into a focal point in front of the retina, and then forms an unclear image on the retina. The distance vision is obviously reduced, but the near vision is still normal. [0003] In recent years, the incidence of myopia has soared, and the proportion of people with myopia has been increasing. In some parts of Asia, nearly 80 percent of young people are nearsighted. Stat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q1/6858C12Q2600/118C12Q2600/156C12Q2521/301C12Q2531/113
Inventor 刘鹏飞田彩娟韩雪姬晓兵
Owner TIANJIN MARVEL BIOTECH
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