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Preparation method of microcapsule for DNA information storage

An information storage and microcapsule technology, applied in the field of information storage, can solve the problem that the storage medium cannot keep up with the growth rate of digital information, and achieve the effect of not being easily disturbed and suppressed, short in time, and large in storage capacity

Pending Publication Date: 2021-01-29
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of electronic technology and the Internet, the resulting explosive growth of digitally stored data has brought great pressure and challenges to data storage methods. The capacity of existing storage media cannot keep up with the growth of digital information. speed, so the traditional data storage method is in urgent need of improvement and breakthrough, and it is of great significance to study an emerging data storage technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] A method for preparing microcapsules for DNA information storage is as follows: SiO 2 The microspheres were evenly dispersed in the solution, and polyethyleneimine PEI solution and deoxyribonucleic acid DNA solution were assembled layer by layer onto SiO by layer-by-layer self-assembly method. 2 On the surface of the microspheres, the outer layer is coated with deoxyribonucleic acid DNA, and the inner layer is coated with polyethyleneimine PEI SiO 2 Microspheres, and then soak the obtained microspheres in the nucleation solution to melt away the SiO 2 The inner core of the microsphere obtains a microcapsule for storing DNA information.

[0019] The outer layer is coated with deoxyribonucleic acid DNA, and the inner layer is coated with polyethyleneimine PEI SiO 2 The preparation method of microsphere comprises the following steps:

[0020] Step 1, the SiO 2 The microspheres are soaked in a positively charged polyethyleneimine PEI solution; after centrifugation to re...

Embodiment 1

[0026] SiO 2 The microspheres were evenly dispersed by shaking, and then the SiO 2 Soak the microspheres in the positively charged PEI solution for about 8-10 minutes, and the concentration of the PEI solution is 1mg / mL; after centrifuging to remove the supernatant, wash it with deionized water for several times (3-5 times), and the centrifugation speed is 8000-9000rpm , centrifugation time 10 ~ 12min. After immersing it in a negatively charged DNA solution for a period of time, the DNA concentration was 1.8 mM. Similarly, soak for 8-10 minutes, then centrifuge to absorb the supernatant, the centrifugation speed and time are the same as above. Repeat the above process several times to obtain SiO with layer-by-layer adsorption of PEI and DNA. 2 Microspheres, and finally soak the obtained microspheres in 10mL fusion nucleation solution to melt away the SiO 2 Microsphere core until the solution is clear and transparent. After centrifugation, remove the supernatant and wash wi...

Embodiment 2

[0028] SiO 2 The microspheres were evenly dispersed by shaking, and then the SiO 2 Soak the microspheres in the positively charged PEI solution for about 4-6 minutes, and the concentration of the PEI solution is 0.5mg / mL; after centrifuging to remove the supernatant, wash it with deionized water several times (3-5 times), and the centrifugation speed is 6000- 7000rpm, centrifugation time 8 ~ 10min. After immersing it in a negatively charged DNA solution for a period of time, the DNA concentration was 0.9 mM. Similarly, soak for 5-7 minutes, then centrifuge to absorb the supernatant, and the centrifugation speed and time are the same as above. Repeat the above process several times to obtain SiO with layer-by-layer adsorption of PEI and DNA. 2 Microspheres, and finally soak the obtained microspheres in 10mL fusion nucleation solution to melt away the SiO 2 Microsphere core until the solution is clear and transparent, centrifuge to remove the supernatant, and then wash with ...

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Abstract

The invention discloses a preparation method of a microcapsule for DNA information storage, belonging to the technical field of information storage. The preparation method comprises the following steps: soaking a silicon dioxide microsphere in a positively charged polyethyleneimine solution, conducting centrifugal cleaning, soaking the silicon dioxide microspher in a negatively charged deoxyribonucleic acid solution, conducting centrifugal cleaning with deionized water for a period of time, repeating the above operations multiple times, assembling PEI and DNA on the surfaces of the silicon dioxide microsphere multiple times, soaking the microsphere having been subjected to layer-by-layer self-assembling into a core melting solution, and dissolving out the core of the silicon dioxide microsphere. Due to the fact that the positively charged PEI solution and the negatively charged DNA solution can be adsorbed through electrostatic interaction, the microcapsule can be prepared through a layer-by-layer self-assembling method. The method is simple and convenient to operate, used materials are cheap and easy to obtain, and preparation time is short.

Description

technical field [0001] The invention belongs to the technical field of information storage, and in particular relates to a preparation method of microcapsules for DNA information storage. Background technique [0002] With the rapid development of electronic technology and the Internet, the resulting explosive growth of digitally stored data has brought great pressure and challenges to data storage methods. The capacity of existing storage media cannot keep up with the growth of digital information. Therefore, the traditional data storage methods are in urgent need of improvement and breakthrough, and it is of great significance to study an emerging data storage technology. [0003] Deoxyribonucleic acid (DNA) is a biological molecule composed of deoxyribose, phosphate, and bases (adenine, thymine, cytosine or guanine), and is an important biological information storage medium. Utilizing DNA for data storage is efficient, stable and resource-saving. [0004] DNA storage is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02G06F16/901C12N15/10
CPCB01J13/02G06F16/901C12N15/10
Inventor 葛丽芹骆晨曦张云起
Owner SOUTHEAST UNIV
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