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Bacillus endophyticus from anti-nematode variety and application of bacillus endophyticus

A Bacillus and plant technology, applied in application, nematicide, plant growth regulator, etc., can solve problems such as affecting practical value and difficult to dominate

Active Publication Date: 2016-07-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The vast majority of plant root-knot nematode biocontrol bacteria are isolated and screened from soil or plant rhizosphere soil microorganisms, but these soil microorganisms are easily affected by external conditions and are difficult to inhabit with soil, plant rhizosphere or leaf peripheries. long-term advantage in the competition of microorganisms, thus greatly affecting its practical value

Method used

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  • Bacillus endophyticus from anti-nematode variety and application of bacillus endophyticus

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Embodiment 1

[0038] Example 1, Isolation and Identification of Bacillus JZB126 in Plants

[0039] 1.1 Strain isolation

[0040] In November 2014, the young roots of nematode-resistant tomatoes (variety: Holland Hard Powder No. 8) were collected from the tomato field of Luao Greenhouse Base in Shunyi District, Beijing, put into ziplock bags, and brought back to the laboratory for processing. Weigh 1 g of young roots, rinse with 75% alcohol for 30 seconds, wash with sterile water three times, cut the young roots into pieces with sterile scissors, put them into a sterilized mortar and add quartz sand for grinding. Add the grinding solution to a centrifuge tube filled with 9 mL of sterile water, and the sample concentration is 10 -1 , shake well, and then make 10 -2 、10 -3 、10 -4 、10 -5Gradient dilution. Draw 200 μL of different gradient dilutions, and evenly spread them on the beef extract peptone plate, and repeat 3 times for each concentration. Placed in a 28°C incubator for cultivat...

Embodiment 2

[0054] Embodiment 2, plant root-knot nematode inhibitor and nematicidal activity thereof

[0055] 1. Cultivation of Bacillus endophyticus JZB126

[0056] Beef extract peptone medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 15.0g agar, distilled water to 1000ml, mix and pack, sterilize at 121°C for 20min. Bacillus endophyticus (Bacillus endophyticus) JZB126 was streaked three times on the beef extract peptone medium plate to obtain a single colony.

[0057] 2. Nematicidal activity of Bacillus endophyticus JZB126

[0058] 2.1 Obtaining eggs, oocysts and second instar larvae of M. incognita

[0059] Meloidogyne incognita was inoculated in indoor pots with the susceptible tomato variety Jiafen No. 15. After 40 days of inoculation, a large number of oocysts appeared in the tomato root system. Gently rinse the root system with water, carefully remove the oocysts, put them in 0.5% sodium hypochlorite solution for disinfection for 3 minutes, and then rinse them with sterile wa...

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Abstract

The invention discloses bacillus endophyticus from an anti-nematode variety and application of the bacillus endophyticus. The bacterial strain number of the bacillus endophyticus is JZB126, and the register number of the bacillus endophyticus in General Microorganism Center, China Committee for Culture Collection of Microorganisms is CGM CC No. 11846. The corrected death rate of the bacillus endophyticus JZB126 for root-knot nematode in the South is 99. 1%, after four generations are cultured continuously, the corrected death rate for root-knot nematode in the South is 97. 1%, the mean ovum incubation inhibition rate for root-knot nematode in the South is 99. 0%, under the condition that no auxiliary agent is added, on the 45 day, the prevention effect for root-knot nematode disease of cucumbers reaches 80. 7%, and 80 days later, the prevention effect for root-knot nematode disease of cucumbers still reaches 65. 1%. The bacillus endophyticus JZB126 and / or mcetabolin thereof can be applied to prevention and treatment of root-knot nematode of plants.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to an intraplant bacillus derived from a nematode-resistant variety and its application. Background technique [0002] Root-knot nematode Meloidogynes pp. is a class of soil-borne pathogen that seriously endangers agricultural production. It can parasitize various crops, especially vegetables, and has caused serious yield reduction in tropical and subtropical regions (KiewnickS, SikoraRA.2006. 38(2):179-187.). In recent years, with the continuous expansion of vegetable planting area in my country, the incidence of vegetable root-knot nematode has increased year by year, and it has become one of the difficult diseases that seriously affect the safety of vegetable products (Zhao Zhixiang et al. Construction of genomic library and screening of a nematicide protease gene. Acta Microbiology, 50(8): 1072-1079). [0003] At present, nematode control is still domin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 董丹刘霆刘伟成张涛涛吴慧玲张殿朋王进福
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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