Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cobra venom nerve growth factor purification and preparation method

A technology of nerve growth factor and cobra venom, applied in the direction of nerve growth factor, peptide preparation method, growth factor/inducing factor, etc., can solve the problems of many influencing factors, poor repeatability, low NGF activity, etc.

Inactive Publication Date: 2016-07-06
GUANGXI MEDICAL UNIVERSITY
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing cobra venom NGF separation and purification mainly adopts the combination of gel column chromatography and ion exchange chromatography, and after separation and purification, methods such as PC12 cell method, two-way immunodiffusion method or SDS-PAGE electrophoresis are used to separate the NGF. The results were verified, but due to the many factors affecting the column efficiency and it is difficult to control, the repeatability of this method is poor, and the activity of the obtained NGF is low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cobra venom nerve growth factor purification and preparation method
  • Cobra venom nerve growth factor purification and preparation method
  • Cobra venom nerve growth factor purification and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1 Materials and methods

[0028] 1.1 Apparatus and reagents Freeze-dried powder of Guangxi cobra venom (provided by the Snake Venom Research Institute of Guangxi Medical University, cobra is the genus Cobra of the family Reptilia, Squama, Snake, and Najaatra, and the Latin name is najaatra), electronic analytical balance (British Sartorius company ), L18-4584-01 system (PharmaciaBiotech, USA), BioLogicDuoFlow system (BIO-RAD, USA), CO 2 Incubator (ThermoForma, USA), 1640 culture medium (Gbico, USA), fetal bovine serum (Wisnt, Canada), horse serum (Hyclone, USA), and other reagents were all domestic analytical grade.

[0029] 1.2 Experimental method

[0030] 1.2.1 PC12 cell culture PC12 cells were treated with 1640 medium containing 10% horse serum and 5% fetal bovine serum in 5% CO 2 , cultured in a 37°C incubator. The PC12 cells in good growth state were inoculated into 48-well plates for later use.

[0031] 1.2.2 Sample preparation Weigh 1 g of cobra venom freeze-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cobra venom nerve growth factor purification and preparation method. Firstly, a DEAE CL-6B anion exchange column is adopted and then a Sephadex G-50 gel chromatography column, a Macro-prep High S cation exchange column is utilized for separation in sequence, a PC12 cell test method is adopted to dilute collected peaks with ultra-pure water after each time of separation, and then testing is performed to select the peaks having the highest cobra venom nerve growth factor activity as samples for subsequent separation, wherein the minimum concentration of NGF induced PC12 cell differentiation is determined through dilution. An inventor finds peaks having the highest NGF activity in the cobra venom separation process and determines that the minimum concentration of the NGF induced PC12 cell differentiation is up to 0.1 micrograms / ml. Tests show that electrophoresed pure NGF can be obtained by applying the method and have high purity and high activity.

Description

technical field [0001] The invention belongs to the field of preparation of snake venom nerve growth factor, in particular to a method for purifying and preparing cobra venom nerve growth factor. Background technique [0002] Nerve growth factor (NGF) is an important nutrient component of the nervous system and plays an important role in ensuring the normal function of the nervous system. In addition, NGF is also closely related to endocrine, immunity, reproductive system, Alzheimer's disease, etc. . Its main function has four aspects: <1> Promote the growth and development of the nervous system during the development of the body; <2> Protect normal nerve cells after the body matures; <3> Nerve damage (such as cerebrovascular accident and brain trauma) ) can promote neuron regeneration and functional recovery; <4> improve learning and memory functions. [0003] Cobra venom nerve growth factor, as the main active ingredient of the drug, has good cura...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/48C07K1/18C07K1/16
CPCC07K14/48
Inventor 张学荣侯瑞雪张可星罗小玲廖明班建东孙林蔡凤桃陈缨农君李洪涛
Owner GUANGXI MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com