Tissue engineering material with biologically active surface layer and preparation method thereof
A biological activity and tissue engineering technology, applied in the field of tissue engineering materials, tissue engineering materials and preparation methods and their preparation fields, can solve problems such as excessive degradation and insufficient mechanical strength, and achieve increased affinity, high mechanical strength, and promotion of adhesion. Effect
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Embodiment 1
[0019] Example 1: This embodiment relates to a tissue engineering material with biologically active groups, the base material of which is polylactic acid (PLA), and chitin active polymer is linked via dopamine.
[0020] The preparation method of the tissue engineering material comprises the following steps:
[0021] Step 1. Disinfect the PLA matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 2 days, and distilled water for 1.5 days to make it fully humidified;
[0022] Step 2, preparing the modification liquid solution, dissolving dopamine in Tris buffer solution, carrying out activation treatment, then adding chitin active polymer to make it fully dissolved and mixed evenly, wherein the concentration of dopamine is 2.5mg / ml, and the concentration of chitin is 3mg / ml;
[0023] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matrix ma...
Embodiment 2
[0026] Example 2: This example relates to a tissue engineering material with biologically active groups, the base material of which is poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV), and chitin active polymer is linked by dopamine .
[0027] The preparation method of the tissue engineering material comprises the following steps:
[0028] Step 1. Disinfect the PHBV matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 3 days, and distilled water for 1 day to make it fully humidified;
[0029] Step 2. Prepare the modification liquid solution, dissolve dopamine in Tris buffer, perform activation treatment, and then add gene peptide gel active polymer to fully dissolve and mix evenly. The concentration of dopamine is 3mg / ml, and the gene peptide Glue concentration is 3mg / ml;
[0030] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matri...
Embodiment 3
[0033] Example 3: This embodiment relates to a tissue engineering material with bioactive groups, the base material of which is polycaprolactone (PCL), and chitin active polymer is connected via dopamine.
[0034] The preparation method of the tissue engineering material comprises the following steps:
[0035] Step 1. Sterilize the PCL matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 3 days, and distilled water for 1 day to make it fully humidified;
[0036] Step 2. Prepare the modification liquid solution, dissolve dopamine in Tris buffer, perform activation treatment, and then add type I collagen active polymer to fully dissolve and mix evenly, wherein the concentration of dopamine is 4mg / ml, and type I collagen gel The concentration is 4mg / ml;
[0037] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matrix material, and incubat...
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Abstract
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