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Evaluation method for tumour model effective intervention based on optical imaging technology

A technology of optical imaging and evaluation method, applied in the field of experimental animal and medical image processing and application, to achieve the effect of overcoming waste of funds, saving costs, and improving comparability

Inactive Publication Date: 2016-07-06
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
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AI Technical Summary

Problems solved by technology

Although acupuncture and moxibustion treatment of tumors are mainly based on strengthening the body and consolidating the root, the principle of acupoint selection is still based on syndrome differentiation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Establishing an Evaluation Method for Effective Intervention of Tumor Models Based on Optical Imaging Technology

[0030] Go through the following steps:

[0031] 1) Preparation of tumor cell lines carrying bioluminescence luciferase gene

[0032] Establish B16 malignant melanoma and 4T1 breast cancer cell lines carrying bioluminescence luciferase gene;

[0033] (1) Construction of a plasmid carrying the Luciferase gene expression cassette

[0034] Digest pGL4.0 (luc) to obtain a 1653bp luc gene fragment, insert the target fragment into the lentiviral vector pRRLsin, and construct the lentiviral vector pRRLsin-luc containing the luc gene;

[0035] (2) Lentiviral packaging part

[0036] Use jetPEI transfection reagent, pRRLsin-luc, lentiviral packaging plasmid Pcmvr8.74, and lentiviral envelope plasmid Pmd2.G to co-transfect 293T cells according to the ratio of 3:2:1. After 48 hours, the virus fluid containing Transfer the culture medium to a 15ml centrifug...

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Abstract

The invention belongs to the technical field of experiment animals and medical image processing and application, and specifically relates to an evaluation method for tumour model effective intervention based on an optical imaging technology. The method comprises: using bioluminescence and fluorescent techniques to mark tumour model cells, using optical detection instruments to directly detect activities of the marked cells of a tumour model and behaviors of marked genes, observing expression or interaction of specific cells, genes, and molecules, and meanwhile detecting a plurality of kinds of molecular events, tracking targets, and imaging and evaluating an intervening measure effect from molecular and cellular level. The method can observe growth and metastasis of tumour cells in vivo and reaction after intervention in real time. Further, the method can be used for simply, conveniently, and accurately screen out effective intervention measures or effective intervention compatibility measures for different histological pattern tumours.

Description

technical field [0001] The invention belongs to the field of experimental animal and medical image processing and application technology, and relates to right An evaluation method for effective intervention of tumor models, specifically relating to an evaluation method for effective intervention of tumor models based on optical imaging technology. Background technique [0002] According to reports, in recent years, with the deterioration of environmental pollution and the continuous changes in people's lifestyles, cancer has seriously threatened human health, and its morbidity and mortality have rapidly jumped to the top of the world. Studies have shown that tumor immunotherapy methods based on immunological principles and immunological techniques have gradually become the first choice for clinical treatment of advanced tumors because they can produce positive anti-cancer effects, have few side effects and cause little damage to the body. It is an important means and is pla...

Claims

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Application Information

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IPC IPC(8): A61B5/00C12N5/10C12N15/866A61K49/00
Inventor 周俊梅张必萌韦芳刘素君刘鹏
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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