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Prawn feed additive and application thereof

A technology for shrimp feed and additives, applied in the application, animal feed, additional food elements, etc., to achieve the effect of reducing mortality, significant economic and social benefits, and broad market prospects

Inactive Publication Date: 2016-07-06
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few reports on the effective immunostimulants for white spot syndrome

Method used

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  • Prawn feed additive and application thereof
  • Prawn feed additive and application thereof
  • Prawn feed additive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the multiplication situation of white spot syndrome virus in the prawn body after artificial infection

[0026] Select Litopenaeus vannamei with a body weight of 3.0±0.5g, and inject 50 μL of 10 8 White spot syndrome virus of copy number (with prawn saline as solvent), 10 tail prawns were randomly selected at each time point in each group, and each group was set up with 3 parallel repetitions, and 6, 24, and 48 hours after injection of white spot syndrome virus were collected. Shrimp gills and muscle tissue were mixed, total RNA was extracted, and the proliferation of white spot syndrome virus was detected by semi-quantitative PCR (see the article published by the applicant's research group for the detection method and primer sequence: DisAquaOrg, 2011, 96:9-20 ). It was found that the prawns used before the artificial infection were negative for white spot baculovirus particles, and all the prawns were infected with virions by injecting only once. As tim...

Embodiment 2

[0027] Embodiment 2, after adding L-arginine to the feed, the change of the mortality rate of Litopenaeus vannamei

[0028] The above-mentioned Litopenaeus vannamei without WSSV infection with a body weight of about 3 g were selected and randomly divided into 4 groups, namely group 1 to group 4, with 3 replicates in each group and 10 prawns in each replicate. The prawn basal feed without adding L-arginine (Guangdong brand, commercially available) is used as a control group (group 1), and 10 g (group 2) and 20 g (group 3) of L-arginine are added in every kilogram of prawn basal feed , 40g (group 4), 80g (group 5) (obtained by adding L-arginine to the commercially available Yuehai brand feed respectively), feeding twice a day, after 7 days of continuous feeding, groups 1 to 4 were fed Infected with white spot syndrome virus dead shrimp muscle challenge virus (prawns have the habit of nibbling sick and weak shrimp or dead shrimp of the same kind, eating sick shrimp infected with ...

Embodiment 3

[0032] Example 3, the effect of artificial injection of L-arginine on the in vivo proliferation of Litopenaeus vannamei against white spot syndrome virus.

[0033] Using the above-mentioned prawns, group 1 is the control group, each prawn is injected with 50 μL of prawn physiological salt solution, and the prawn white spot syndrome virus group is injected, and group 2 is the experimental group, and each prawn is injected with 0.4 mg / g body weight of L-arginine (dissolved in 50 μL prawn physiological saline solution); 24 hours after the injection of group 1 and group 2, artificial injection of 10 8 White spot syndrome virus particles (dissolved in 50 μL prawn physiological salt solution) of the copy number were used to challenge the virus, and the gills and tissues of the prawns were collected 6h, 24h and 48h after the challenge, and the presence of white spot syndrome virus in the shrimp was detected by semi-quantitative PCR. Proliferation (the detection method and the primers...

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Abstract

The invention provides a prawn feed additive and application thereof, and relates to a feed additive.The prawn feed additive is L-arginine and has the Chinese nicknames of arginine, L-2-amino-5-guanidinovaleric acid, guanidinovaleric acid and L-guanidinovaleric acid; the molecular formula of L-arginine is C6H14N4O2; the molecular weight of L-arginine is 174.20.The prawn feed additive can be applied to preparation of feed for resisting a prawn white spot syndrome virus.The prawn feed additive L-arginine is added into prawn feed to be premixed and then dried at the temperature of 50 DEG C-60 DEG C, and then the feed for resisting the prawn white spot syndrome virus is obtained.Through experiments, it is verified that the death rate of prawns attacked by the white spot syndrome virus can be effectively decreased by adding L-arginine in the feed, and the multiplication speed of the prawn white spot syndrome virus in prawn bodies can be significantly inhibited by injecting L-arginine.

Description

technical field [0001] The invention relates to a feed additive, in particular to a prawn feed additive and its application. Background technique [0002] Shrimp white spot syndrome virus (Whitespotsyndromevirus, WSSV) is a double-stranded DNA virus. It mainly infects and parasitizes important systems such as gills, muscles, hematopoietic tissues, stomach, intestines, blood cells, heart, eyes, and nerves of prawns, causing organ and tissue lesions, and even systemic necrosis. The infection rate of this disease is extremely high, and it is also the virus that causes the highest mortality rate of cultured Litopenaeus vannamei. Due to the strict cell parasitic characteristics of the virus, there is no effective chemical drug to prevent and treat the disease caused by white spot syndrome virus in shrimp. It is the most feasible way to prevent white spot syndrome virus infection in production through feed administration, but At present, there are not many feed additives that ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/142
Inventor 姚翠鸾胡文燕
Owner JIMEI UNIV
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