Prawn feed additive and application thereof
A technology for shrimp feed and additives, applied in the application, animal feed, additional food elements, etc., to achieve the effect of reducing mortality, significant economic and social benefits, and broad market prospects
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Embodiment 1
[0025] Embodiment 1, the multiplication situation of white spot syndrome virus in the prawn body after artificial infection
[0026] Select Litopenaeus vannamei with a body weight of 3.0±0.5g, and inject 50 μL of 10 8 White spot syndrome virus of copy number (with prawn saline as solvent), 10 tail prawns were randomly selected at each time point in each group, and each group was set up with 3 parallel repetitions, and 6, 24, and 48 hours after injection of white spot syndrome virus were collected. Shrimp gills and muscle tissue were mixed, total RNA was extracted, and the proliferation of white spot syndrome virus was detected by semi-quantitative PCR (see the article published by the applicant's research group for the detection method and primer sequence: DisAquaOrg, 2011, 96:9-20 ). It was found that the prawns used before the artificial infection were negative for white spot baculovirus particles, and all the prawns were infected with virions by injecting only once. As tim...
Embodiment 2
[0027] Embodiment 2, after adding L-arginine to the feed, the change of the mortality rate of Litopenaeus vannamei
[0028] The above-mentioned Litopenaeus vannamei without WSSV infection with a body weight of about 3 g were selected and randomly divided into 4 groups, namely group 1 to group 4, with 3 replicates in each group and 10 prawns in each replicate. The prawn basal feed without adding L-arginine (Guangdong brand, commercially available) is used as a control group (group 1), and 10 g (group 2) and 20 g (group 3) of L-arginine are added in every kilogram of prawn basal feed , 40g (group 4), 80g (group 5) (obtained by adding L-arginine to the commercially available Yuehai brand feed respectively), feeding twice a day, after 7 days of continuous feeding, groups 1 to 4 were fed Infected with white spot syndrome virus dead shrimp muscle challenge virus (prawns have the habit of nibbling sick and weak shrimp or dead shrimp of the same kind, eating sick shrimp infected with ...
Embodiment 3
[0032] Example 3, the effect of artificial injection of L-arginine on the in vivo proliferation of Litopenaeus vannamei against white spot syndrome virus.
[0033] Using the above-mentioned prawns, group 1 is the control group, each prawn is injected with 50 μL of prawn physiological salt solution, and the prawn white spot syndrome virus group is injected, and group 2 is the experimental group, and each prawn is injected with 0.4 mg / g body weight of L-arginine (dissolved in 50 μL prawn physiological saline solution); 24 hours after the injection of group 1 and group 2, artificial injection of 10 8 White spot syndrome virus particles (dissolved in 50 μL prawn physiological salt solution) of the copy number were used to challenge the virus, and the gills and tissues of the prawns were collected 6h, 24h and 48h after the challenge, and the presence of white spot syndrome virus in the shrimp was detected by semi-quantitative PCR. Proliferation (the detection method and the primers...
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