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Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components

A technology of carotene and capsicum, which is applied in the field of extraction of carotenoid components in capsicum leaves and quantitative detection of its content, can solve problems such as strong limitations and no industry measurement standards, and achieve a good linear relationship and convenient carotenoids Element components, method scientific and feasible effect

Active Publication Date: 2016-06-29
长治市全民大健康产业研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prior art provides an ultra-high performance liquid chromatography assay method for carotenoid content in wheat, which can completely separate lutein and zeaxanthin in wheat, but this method is limited to lutein, zeaxanthin quality, β-carotene, α-carotene, the extraction and determination of the content of 4 carotenoids; the prior art also provides a method for the determination of the content of strawberry carotenoids, using ultra-high performance liquid chromatography Determination of lutein, β-carotene, β-cryptoxanthin and lycopene in strawberry fruit, but with strong limitations
At present, there are few studies on the determination of carotenoids by reversed-phase high-performance liquid chromatography. In the existing studies, no method is suitable for all test materials. However, the separation and content determination of various carotenoid components in pepper leaves The method has not been reported in the literature at present, and there is no unified industry measurement standard

Method used

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  • Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components
  • Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components
  • Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The extraction method of carotenoids in pepper leaves is as follows:

[0063] 1) Grind in an ice bath, weigh 2.000g of the divided pepper leaf samples, add 0.010g of quartz sand and 0.1000g of antioxidant 2,6-di-tert-butyl-p-cresol (BHT), and add liquid nitrogen to the mortar Quickly grind, fully grind to obtain pepper leaf powder;

[0064] 2) Saponification: Place the pepper leaf powder ground in the ice bath in step 1) into a 50mL dark brown reagent bottle, add 8mL KOH-methanol saponification solution with a mass concentration of 20%, seal it and place it in a constant temperature water bath at 55°C for 30min. Then immediately cool to room temperature with cold water;

[0065] 3) Extraction: Add 16 ml (solid-liquid ratio of 1:8) mixed extraction solvent of acetone-ethyl acetate (V:V=1:2) to the saponified pepper leaves obtained in step 2), extract by ultrasonic for 40 min, and ultrasonically extract The temperature is 30℃;

[0066] 4) Centrifugation: centrifuge the...

Embodiment 2

[0069] The extraction method of carotenoids in pepper leaves is as follows:

[0070] 1) Grind in an ice bath, weigh 2.000g of the divided pepper leaf samples, add 0.010g of quartz sand and 0.1000g of antioxidant 2,6-di-tert-butyl-p-cresol (BHT), and add liquid nitrogen to the mortar Quickly grind, fully grind to obtain pepper leaf powder;

[0071] 2) Saponification: Place the pepper leaf powder ground in the ice bath in step 1) into a 50mL dark brown reagent bottle, add 12mL KOH-methanol saponification solution with a mass concentration of 15%, seal it and place it in a constant temperature water bath at 80°C for 20min. Then immediately cool to room temperature with cold water;

[0072] 3) Extraction: add 4 ml (solid-liquid ratio of 1:2) mixed extraction solvent of acetone-ethyl acetate (V:V=1:2) to the saponified pepper leaves obtained in step 2), extract by ultrasonic for 10 minutes, and ultrasonically extract The temperature is 30℃;

[0073] 4) Centrifugation: centrifuge...

Embodiment 3

[0076] The extraction method of carotenoids in pepper leaves is as follows:

[0077] 1) Grind in an ice bath, weigh 2.000g of the divided pepper leaf samples, add 0.010g of quartz sand and 0.1000g of antioxidant 2,6-di-tert-butyl-p-cresol (BHT), and add liquid nitrogen to the mortar Quickly grind, fully grind to obtain pepper leaf powder;

[0078] 2) Saponification: Place the pepper leaf powder ground in the ice bath in step 1) into a 50 mL light-proof brown reagent bottle, add 4 mL of KOH-methanol saponification solution with a mass concentration of 30%, seal it and place it in a constant temperature water bath at 40°C for 60 min. Then immediately cool to room temperature with cold water;

[0079] 3) Extraction: add 12 ml (solid-liquid ratio of 1:6) mixed extraction solvent of acetone-ethyl acetate (V:V=1:2) to the saponified pepper leaves obtained in step 2), extract by ultrasonic for 50 min, and ultrasonically extract The temperature is 30℃;

[0080] 4) Centrifugation: c...

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Abstract

The invention provides a method for extracting carotenoid components in pepper leaves. The method includes the steps that 1, liquid nitrogen is added into the fresh pepper leaves, and the fresh pepper leaves are fully ground into powder; 2, a KOH-methanol saponifying solution with the mass concentration of 15-30% is added into the pepper leaves to carry out saponification for 20-60 min at the temperature of 40-80 DEG C; 3, an acetone-ethyl acetate mixed extracting solvent is added into the saponified pepper leaves prepared in the step 2 to conduct supersonic extraction to obtain a carotenoid crude extract; 4, the carotenoid crude extract obtained in the step 3 is subjected to aftertreatment to obtain a solution containing carotenoid. The invention further provides a high performance liquid chromatography detection method for the carotenoid components, 10 kinds of carotenoid in the pepper leaves can be separated well, and the content of the 10 kinds of carotenoid can be quantitatively measured at the same time.

Description

technical field [0001] The invention relates to the extraction of ten carotenoid components in pepper leaves and a method for quantitative detection of the content by reverse-phase high-performance liquid chromatography. Background technique [0002] chili( Capsicumannuum.L. ), Solanaceae capsicum is an annual herbaceous plant. There are cultivation records in my country more than 400 years ago. At present, there are dozens of cultivars, which are planted all over the country. They are also the main vegetables cultivated in winter and spring in the northern region. Varieties, protected cultivation in winter and spring in the northern region is mainly affected by low temperature and weak light, which in turn affects the yield and quality of peppers. Screening out pepper varieties with strong resistance to low light and low temperature and low light can fundamentally solve the problem. [0003] Carotenoids are a class of natural pigments that widely exist in nature, with vario...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 颉建明郁继华吕剑李静李杰王小龙胡玉琴
Owner 长治市全民大健康产业研究院
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