Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Technology for enhancing fluorescence and improving anti-oxidant activity of DNA-Ag NCs

A technology of antioxidant activity and silver nanoclusters, applied in nanotechnology and other fields

Active Publication Date: 2016-06-29
CENT SOUTH UNIV
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The improvement of the antioxidant activity of DNA-AgNCs is mainly achieved through the precise design of template molecules, but there are relatively few reports that can simultaneously enhance the fluorescence and antioxidant activity of DNA-AgNCs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Synthesis of silver nanoparticles stabilized by sodium citrate: 1mL, 40mM trisodium citrate solution and 15mM silver nitrate solution were mixed in equal volumes under dark conditions, then added secondary water to make the volume to 50mL, and kept stirring for 30min. Then quickly add 1mL, 15mM fresh NaBH 4 The ice-water mixture was subjected to reduction treatment, and the mixture was continuously shaken at room temperature for 3 hours. Transfer to a 4°C refrigerator to stand for use.

[0012] Synthesis of DNA-AgNCs: Under dark conditions, 127 μL of 10 μM cytosine-rich oligonucleotide was mixed with 60 μM, 12.7 μL of silver nitrate solution and stirred continuously for 30 min, then quickly added 60 μM, 12.7 μL of freshly prepared NaBH 4 The ice-water mixture was subjected to reduction treatment, and the mixture was continuously shaken at room temperature for 2 hours.

[0013] 100 μL of the above DNA-AgNCs aqueous solution was mixed with silver nanoparticles stabilize...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a technology for enhancing fluorescence and improving anti-oxidant activity of DNA-Ag NCs synthesized by the DNA template method. The fluorescence intensity of the DNA-Ag NCs synthesized by taking a cytosine-rich oligonucleotide as a template is remarkably enhanced in the presence of Ag nano-particles with stable sodium citrate, and the anti-oxidant activity of the DNA-Ag NCs in saturated dissolved oxygen is also improved obviously. The technology provided by the invention has the advantages of simple operation, high speed and efficiency, low cost and the like.

Description

technical field [0001] The invention relates to a process for enhancing the fluorescence of silver nanoclusters and improving their antioxidant activity, in particular to a process for enhancing the fluorescence of silver nanoclusters synthesized with DNA as a template and improving their antioxidant activity. Background technique [0002] Silver nanoclusters (DNA-AgNCs) synthesized by DNA as a template have the characteristics of wide emission wavelength range, good biocompatibility and excellent antibacterial performance, and have been widely used in biosensing and cell imaging and other fields. However, silver nanoclusters emit blue light because they are easily oxidized by air or oxidants in the reaction system, and the fluorescence intensity is relatively low. Therefore, it is necessary to develop a process to effectively enhance the fluorescence intensity of DNA-AgNCs and improve their antioxidant activity. [0003] At present, the process of enhancing the fluorescenc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB22F9/24B82Y40/00
Inventor 王建秀胡盛强衣馨瑶叶宝玉胡蓝双羿甜甜
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com