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Test method for radix rehmanniae in scourge-clearing toxin-vanquishing powder

A technology of Qingwenbaidu powder and detection method, applied in the field of detection, can solve problems such as poor effect, and achieve the effect of excellent development performance, remarkable effect and excellent spot separation degree.

Active Publication Date: 2016-06-22
SICHUAN DECHENG ANIMAL HEALTH PROD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: in the prior art, the thin-layer identification method for rehmannia glutinosa used in Qingwenbaidu powder has poor effect, and the purpose is to provide a detection method for rehmannia glutinosa in Qingwen Baidu powder with excellent detection effect

Method used

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  • Test method for radix rehmanniae in scourge-clearing toxin-vanquishing powder

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Effect test

Embodiment 1

[0028] The detection method used for rehmannia glutinosa in Qingwenbaidu powder comprises the following steps:

[0029] (1) Use Qingwenbaidu powder, Rehmannia glutinosa, and Qingwen Baidu powder excluding rehmannia to prepare the test solution, control medicinal material solution, and negative sample solution; the specific preparation process is as follows:

[0030] Take 2g of Qingwenbaidu powder, add 25ml of methanol, sonicate for 30min, filter, evaporate the filtrate to dryness, add 10ml of water to the residue to dissolve, then shake and extract twice with ethyl acetate, 10mL each time, combine the ethyl acetate solution, evaporate Dry, add methanol 2mL to dissolve the residue, make the test solution;

[0031] Take 1 g of the reference drug Rehmannia glutinosa, add 25 ml of methanol, sonicate for 30 min, filter, evaporate the filtrate to dryness, add 10 ml of water to the residue to dissolve, then shake and extract twice with ethyl acetate, 10 mL each time, combine the ethy...

Embodiment 2

[0037] This embodiment is a comparative example of embodiment 1. In this embodiment, the composition and ratio of the developing agent are different. In this embodiment, the developing agent adopts dichloromethane, methanol and ammonia water. The dichloromethane, methanol and ammonia water The ratio is 12:1:0.1.

[0038] The test results of the developer are as follows: figure 2 As shown, from left to right in the picture are the control medicinal material solution, the test solution, and the negative sample solution. When using the developer at this ratio, it is found that the negative has interference, and the effect is not ideal, which greatly affects the accuracy of the detection effect. degree of judgment.

Embodiment 3

[0040] This embodiment is a comparative example of embodiment 1. In this embodiment, the composition and ratio of the developing agent are different. In the present embodiment, the developing agent adopts chloroform, methanol and ammonia water, and the chloroform, methanol and ammonia water The ratio is 12:1:0.1. Using the developing agent of this embodiment, the detection results are as follows: image 3 As shown, from left to right are the control drug solution, the test solution, and the negative sample solution, each solution is two. When using the developer at this ratio, the polarity of the developer is too high, and the resolution is not good.

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Abstract

The invention discloses a test method for radix rehmanniae in scourge-clearing toxin-vanquishing powder.The problem that a thin-layer identification method for radix rehmanniae in the scourge-clearing toxin-vanquishing powder has poor effect in the prior art is solved.The test method includes: (1) preparing test solution with the scourge-clearing toxin-vanquishing powder; (2) sucking the test solution prior to dropwise adding the test solution onto a thin-layer silica gel plate, and taking trichloromethane, methyl alcohol and ammonia water in the proportion of 14:1:0.1 as a developer for developing prior to taking out and airing; (3) spraying vanillin-sulfuric acid solution prior to heating till spots become clear.The test method has the advantages that developing performance is excellent, other components in the scourge-clearing toxin-vanquishing powder have no influence on the radix rehmanniae test effect, the degree of spot separation in the map is extraordinary, and the like.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for rehmannia glutinosa in Qingwenbaidu powder. Background technique [0002] Qingwenbaidu San is a famous prescription created by Yu Shiyu, a famous febrile disease scientist in the Qing Dynasty. It is contained in the book "Epidemic Disease Yide" written by him, and it is now included in the second volume of the 2010 edition of "The People's Republic of China Veterinary Pharmacopoeia" . This medicine is composed of 14 medicinal materials such as gypsum, rehmannia glutinosa, buffalo horn, and coptis. The prescription of Qingwenbaidu powder includes: raw gypsum 120g, rehmannia 30g, buffalo horn 60g, coptis 20g, gardenia 30g, moutan cortex 20g, scutellaria baicalensis 25g, red peony root 25g, scrophulariaceae 25g, anemarrhena 30g, forsythia 30g, Platycodon grandiflorum 25g, licorice 15g, and bamboo leaves 25g; it has the effect of purging fire, detoxification and cooling ...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 潘春晖刘涛杨琼张煊
Owner SICHUAN DECHENG ANIMAL HEALTH PROD
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