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Method for detecting rhizoma coptidis in powder with effects of clearing acute communicable diseases and counteracting toxin

A technology of Qingwenbaidu powder and a detection method, which is applied in the detection field, can solve problems such as poor effect, and achieve the effect of excellent development performance, remarkable effect, and good separation of spots

Inactive Publication Date: 2016-06-01
SICHUAN DECHENG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: in the prior art, the thin-layer identification method of Coptis chinensis used in Qingwen Baidu powder has poor effect, and the purpose is to provide a detection method for Coptis chinensis in Qingwen Baidu powder with excellent detection effect

Method used

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  • Method for detecting rhizoma coptidis in powder with effects of clearing acute communicable diseases and counteracting toxin
  • Method for detecting rhizoma coptidis in powder with effects of clearing acute communicable diseases and counteracting toxin
  • Method for detecting rhizoma coptidis in powder with effects of clearing acute communicable diseases and counteracting toxin

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for detecting Coptis Rhizoma in Qingwenbaidu Powder, comprising the following steps:

[0028] (1) Use Qingwenbaidu powder, the prescription of Qingwen Baidu powder excluding Coptis chinensis, and Coptis chinensis to prepare the test solution, negative sample solution and control drug solution respectively; the specific preparation is as follows:

[0029] Take 2g of Qingwenbaidu Powder, add 25ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue to dissolve it and make the test solution;

[0030] Take 0.25g of Coptidis rhizome as the reference drug, add 25ml of methanol, sonicate for 30min, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue and dissolve it to make the reference drug solution;

[0031] Take 1 / 500 of the prescription amount of Qingwenbaidu Powder, add 25ml of methanol, ultrasonicate for 30min, filter, evaporate the filtrate to dryness, add 2ml of methanol t...

Embodiment 2

[0036] This embodiment is a comparative example of Example 1. In this embodiment, the ratio of the developer is different. In this embodiment, the ratio of n-butanol, glacial acetic acid and water in the developer is 7:1:2.

[0037] The test results of the developer are as follows: figure 2 As shown, when the developing agent at this ratio is tested, it is found that the bright yellow spots overlap with each other, which greatly affects the judgment of the accuracy of the detection effect.

Embodiment 3

[0039] This embodiment is a comparative example of Example 1. In this embodiment, the ratio of the developer is different. In this embodiment, the ratio of n-butanol, glacial acetic acid and water in the developer is 11:1:2.

[0040] The test results of the developer are as follows: image 3 As shown, the bright yellow spots cannot be completely separated when using the developer at this ratio.

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Abstract

The invention discloses a method for detecting rhizoma coptidis in powder with effects of clearing acute communicable diseases and counteracting toxin. By the aid of the method, the problem of poor effects of methods for identifying rhizoma coptidis thin layers in powder with effects of clearing acute communicable diseases and counteracting toxin in the prior art can be solved. The method includes (1), preparing test solution by the aid of the powder with the effects of clearing the acute communicable diseases and clearing the toxin; (2) sucking the test solution, dripping the test solution on silica gel thin-layer plates, developing the test solution by the aid of butanol, glacial acetic acid and water according to a proportion of 10:1:2, fetching the silica gel thin-layer plates and drying the test solution in the air; (3), visually inspecting the test solution under ultraviolet lamps. The butanol, the glacial acetic acid and the water are used as developing solvents. The method has the advantages that the developing performance is excellent, rhizoma coptidis detection effects are not affected by other components in the powder with the effects of clearing the acute communicable diseases and counteracting the toxin, spots are excellent in resolution under the condition of visual inspection by the aid of the ultraviolet lamps, and the like.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of coptis in Qingwenbaidu powder. Background technique [0002] Qingwenbaidu San is a famous prescription created by Yu Shiyu, a famous febrile disease scientist in the Qing Dynasty. It is contained in the book "Epidemic Disease Yide" written by him, and it is now included in the second volume of the 2010 edition of "The People's Republic of China Veterinary Pharmacopoeia" . This medicine is composed of 14 medicinal materials such as gypsum, rehmannia glutinosa, buffalo horn, and coptis. The prescription of Qingwenbaidu powder includes: raw gypsum 120g, rehmannia 30g, buffalo horn 60g, coptis 20g, gardenia 30g, moutan cortex 20g, scutellaria baicalensis 25g, red peony root 25g, scrophulariaceae 25g, anemarrhena 30g, forsythia 30g, Platycodon grandiflorum 25g, licorice 15g, and bamboo leaves 25g; it has the effect of purging fire, detoxification and cooling blood. It is m...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 潘春晖刘涛杨琼张煊张会峰
Owner SICHUAN DECHENG ANIMAL HEALTH PROD
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