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TLC Identification of Motherwort in Yixuan Granules

A thin-layer identification and Yixuan technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as unsatisfactory repeatability, poor spot separation, and inconspicuous spots, achieving good spot separation and short development time , the effect of simple operation

Active Publication Date: 2017-09-29
广西万寿堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention patent with the notification number CN102937635A discloses a thin-layer identification method of motherwort in Yixuean granules. The pretreatment method of Yixuean granules is to take 15g of finished Yixuean granules, add 100-300ml of cold absolute ethanol, shake Shake and extract for 30 minutes, filter, evaporate the filtrate to dryness, add 2 to 4 ml of absolute ethanol to dissolve the residue, and then use a mixed solution of acetone, absolute ethanol, and concentrated hydrochloric acid with a volume ratio of 6 to 8:4 to 6:1 as the developing solution. In this method, aiming at the imperfect quality standard of the existing Yixuan granules, a mixed solution of acetone, absolute ethanol, and concentrated hydrochloric acid is used as a developing agent to identify the Motherwort in Yixuan granules, but in the actual operation experiment , due to the instability of stachydrine hydrochloride, the development time of the thin-layer identification test is long and the development effect is not ideal, the spots are not well separated or the spots are not obvious, and the repeatability is not ideal. Therefore, there is a lack of a stable Yi Rapid Qualitative Method for Motherwort in Xue'an Granules

Method used

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  • TLC Identification of Motherwort in Yixuan Granules
  • TLC Identification of Motherwort in Yixuan Granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of the test solution: Take 15g of Yixuan granules of three different batch numbers, grind to 100-300 mesh, add 50ml of absolute ethanol, shake and extract for 30 minutes, filter, evaporate the filtrate to dryness, add anhydrous Dissolve in 1ml of ethanol as the test solution.

[0021] Preparation of the reference solution: Dissolve 0.1mg of stachydrine hydrochloride in every 1ml of absolute ethanol.

[0022] Preparation of negative control sample solution: Yixuean granules lacking motherwort, prepared according to the preparation method of the test solution.

[0023] According to the thin-layer chromatography (Appendix VI B of the Chinese Pharmacopoeia 2010 edition), draw 10 μL each of the above-mentioned test solution and reference solution, and respectively spot on the same thin layer of silica gel GF254 with carboxymethylcellulose sodium as the binder. On the plate, use the mixed solution of acetone, ethyl acetate and hydrochloric acid with a volume rati...

Embodiment 2

[0026] Preparation of the test solution: take 15g of Yixuan granules, grind to 100-300 mesh, add 50ml of absolute ethanol, shake and extract for 30 minutes, filter, evaporate the filtrate to dryness, add 1ml of absolute ethanol to dissolve the residue, and use as The test solution.

[0027] Preparation of the reference solution: Dissolve 0.1mg of stachydrine hydrochloride in every 1ml of absolute ethanol.

[0028] According to the thin-layer chromatography (Appendix VI B of the Chinese Pharmacopoeia 2010 edition), draw 10 μL each of the above-mentioned test solution and reference solution, and respectively spot on the same thin layer of silica gel GF254 with carboxymethylcellulose sodium as the binder. On the board, use the mixed solution of acetone, ethyl acetate and hydrochloric acid with a volume ratio of 5:7:1 as the developer, place it in a developing cylinder pre-saturated with the developer, develop it, take it out, dry it, and spray it with modified bismuth iodide Pot...

Embodiment 3

[0031] Preparation of the test solution: take 15g of Yixuan granules, grind to 100-300 mesh, add 50ml of absolute ethanol, shake and extract for 30 minutes, filter, evaporate the filtrate to dryness, add 1ml of absolute ethanol to dissolve the residue, and use as The test solution.

[0032] Preparation of the reference solution: Dissolve 0.1mg of stachydrine hydrochloride in every 1ml of absolute ethanol.

[0033] According to the thin-layer chromatography (Appendix VI B of the Chinese Pharmacopoeia 2010 edition), draw 10 μL each of the above-mentioned test solution and reference solution, and respectively spot on the same thin layer of silica gel GF254 with carboxymethylcellulose sodium as the binder. On the board, use the mixed solution of acetone, ethyl acetate and hydrochloric acid with a volume ratio of 5:7:1 as the developer, place it in a developing cylinder pre-saturated with the developer, develop it, take it out, dry it, and spray it with modified bismuth iodide Pot...

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Abstract

The invention discloses a thin-layer identification method for leonurus japonicus houtt. in Yixuean granules. Stachydrine hydrochloride is adopted as a reference, and acetone, ethyl acetate and hydrochloric acid are adopted as developers. The method has good separation effects, and is high in reproducibility and easy to operate, leonurus japonicus houtt. can be rapidly and qualitatively analyzed, and effective control over the quality of the Yixuean granules is reliably guaranteed.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a thin-layer identification method for motherwort in Yixuan granules. Background technique [0002] Yixue'an Granules is a Chinese patent medicine for treating gynecological anemia. Clinically, it is used for persistent postpartum lochia, unclean uterine bleeding after artificial abortion, and blood stasis syndrome in TCM. [0003] The minister drug of Yixuean Granules is Motherwort, which is the fresh or dry aerial part of Motherwort [Leonurus japonicus Houtt.] of Lamiaceae; it tastes pungent, slightly bitter, and slightly cold in nature, enters the pericardium and liver meridian, and has the function of promoting blood circulation and regulating menstruation. , The effect of diuresis and swelling. The main chemical components in motherwort are alkaloids, flavonoids, diterpenoids, phenylpropanol glycosides, fatty acids, volatile oils, cyclic polypeptides, etc., and contain zinc, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 李修善毛金玲农常东雷飞风
Owner 广西万寿堂药业有限公司
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