Thin-layer chromatography identification method for distinguishing antrodia camphorata and camphorwood

A technology of thin-layer chromatography and cinnamomum, which is applied in the field of TLC identification of Antrodia cinnamomea and cinnamomum cinnamomea, can solve problems such as inability to visually distinguish cinnamomea cinnamomea and cinnamomum cinnamomea, inability to reflect the quality of Antrodia cinnamomea well, and cumbersome operation, etc., to achieve detection Intuitive result, low cost, good effect

Pending Publication Date: 2022-05-13
SHAANXI JIAHE PHYTOCHEM
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the existing research method of liquid phase fingerprint identification of Antrodia cinnamomea, the spectrum is single, the operation is cumbersome, the quality of Antrodia cinnamomea cannot be well reflected, and the technical problem that Antrodia cinnamomea cannot be visually distinguished from Antrodia cinnamomea, while Provide a thin-layer chromatography identification method for distinguishing Antrodia cinnamomea and Cinnamomum camphora

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thin-layer chromatography identification method for distinguishing antrodia camphorata and camphorwood
  • Thin-layer chromatography identification method for distinguishing antrodia camphorata and camphorwood
  • Thin-layer chromatography identification method for distinguishing antrodia camphorata and camphorwood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Preparation of sample solution

[0039] Take the raw materials of Antrodia camphorata and Cinnamomum camphora, pass through 80 mesh sieves after being pulverized, place them in the triangle flasks with stoppers respectively, then add ethanol with 6 times the quality of the raw materials of Antrodia camphorata and Cinnamomum camphorae, and have a mass concentration of 70%, and perform ultrasonic extraction for 60 Minutes, let it cool to room temperature, then filter through a microporous membrane, and collect the filtrate as the sample solution. In order to ensure the accuracy of the results, two samples of Antrodia Cinnamomea and Cinnamomum camphora were prepared respectively, and the serial numbers of Antrodia Cinnamomea and Cinnamomum camphora samples were respectively marked as No. 1 and No. 2;

[0040] 2) Perform thin layer spotting

[0041] The sample solution prepared in step 1) is sequentially placed on the same silica gel G thin-layer plate at different posi...

Embodiment 2

[0056] 1) Preparation of sample solution

[0057] Take the raw materials of Antrodia Cinnamomea and Cinnamomum camphora, pass through an 80-mesh sieve after being crushed, place them in a conical flask with a stopper, then add ethanol with 8 times the mass of Antrodia Cinnamomea and Cinnamomum camphora in a mass concentration of 100%, and perform ultrasonic extraction After 60 minutes, let it cool to room temperature, then filter it with a microporous membrane, and collect the filtrate as the sample solution. In order to ensure the accuracy of the results, two samples of Antrodia Cinnamomea and Cinnamomum camphora were prepared respectively, and the serial numbers of Antrodia Cinnamomea and Cinnamomum camphora samples were respectively marked as No. 1 and No. 2;

[0058] 2) Perform thin layer spotting

[0059] The sample solution prepared in step 1) is sequentially placed on the same silica gel G thin-layer plate at different positions at the same height to form four spotting...

Embodiment 3

[0069] 1) Preparation of sample solution

[0070] Take the raw materials of Antrodia Cinnamomea and Cinnamomum camphora, pass through an 80-mesh sieve after being crushed, place them in a stoppered conical flask, add 6 times the amount of Antrodia Cinnamomum and Cinnamomum camphora raw materials, and ethanol with a mass concentration of 90%, and perform ultrasonic extraction After 30 minutes, let it cool to room temperature, then filter it with a microporous membrane, and collect the filtrate as the sample solution. In order to ensure the accuracy of the results, two samples of Antrodia Cinnamomea and Cinnamomum camphora were prepared respectively, and the serial numbers of Antrodia Cinnamomea and Cinnamomum camphora samples were respectively marked as No. 1 and No. 2;

[0071] 2) Perform thin layer spotting

[0072] The sample solution prepared in step 1) is sequentially placed on the same silica gel G thin-layer plate at different positions at the same height to form four s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a thin-layer chromatography analysis method, in particular to a thin-layer chromatography identification method for distinguishing antrodia camphorata and camphorwood. The technical problems that an existing liquid-phase fingerprint spectrum identification research method for antrodia camphorata is single in spectrum, tedious in operation and incapable of visually distinguishing antrodia camphorata and cinnamomum camphora wood are solved. The method comprises the following steps: 1) preparing a sample solution; 2) carrying out thin-layer sample application; 3) preparing a developing solvent; 4) unfolding; and 5) developing and identifying. The thin-layer chromatography identification method has the advantages of being rapid, low in cost, simple, convenient, efficient, high in specificity, visual in detection result, easy to identify and the like, the antrodia camphorata and the cinnamomum camphora wood can be rapidly identified and distinguished, and the quality of the antrodia camphorata is effectively guaranteed; meanwhile, the blank that thin-layer chromatography research on the antrodia camphorata and the cinnamomum camphora does not exist at present is filled up.

Description

technical field [0001] The invention relates to a thin-layer chromatographic analysis method, in particular to a thin-layer chromatographic identification method for distinguishing antrodia camphorata and camphor wood. Background technique [0002] Antrodia camphorata (Antrodia camphorata), also known as Antrodia camphorata, Antrodia camphorata, Antrodia camphorata, and Zhangku inner mushroom, usually grows saprophytically in the hollow trunks of camphor trees that are hundreds of years old. The camphor tree is the only host of Antrodia camphorata, and the areas suitable for growth are extremely limited, resulting in the scarcity of wild Antrodia camphorata resources. Antrodia is a very rare medicinal fungus. Studies have shown that Antrodia has various pharmacological effects, such as anti-tumor, anti-inflammation, anti-oxidation, immune regulation, and liver protection. It can also be used to treat food and drug poisoning, diarrhea, and abdominal pain , high blood pressur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N1/28G01N1/34
CPCG01N30/90G01N30/94G01N1/28G01N1/34
Inventor 高娟徐沛沛惠玉虎王春德樊艳红韦博
Owner SHAANXI JIAHE PHYTOCHEM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap