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Streptomyces actuosus LB-16 and method for preparing nosiheptide from same

An active Streptomyces, LB-16 technology, applied in microorganism-based methods, biochemical equipment and methods, mutant preparation, etc., can solve the problem of no extraction method, low yield of nosiheptide, unsuitable for industrialization, etc. problems, to achieve the effect of improving the generation rate, improving the efficiency of separation and purification, and meeting the needs of production

Active Publication Date: 2016-06-22
西藏天虹科技股份有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The method of producing nosiheptide is mainly fermentation method. The patent application number 201310027757.5 provides a method of using cysteine ​​to increase the production of nosiheptide, but the method is relatively simple and no extraction method is given. As an industrialized large-scale production, there are certain difficulties
The patent application number 201210469137.2 discloses a method of using active streptozyme bacteria to connect to the slant medium to obtain seed liquid, and then fermenting and culturing to obtain nosiheptide. Although the method is simple to operate, the yield of nosiheptide is low. , also not suitable for industrialization

Method used

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  • Streptomyces actuosus LB-16 and method for preparing nosiheptide from same

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Effect test

Embodiment 1

[0039] An active Streptomyces actuosus LB-16, which is classified and named as Active Streptomycesactuosus, and the active Streptomyces LB-16 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is: CGMCCNo.11732, and the preservation time For: November 25, 2015, the address of the preservation unit is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences.

Embodiment 2

[0041] A method for producing described active streptomyces actuosus LB-16, comprising the steps:

[0042] Step 1, irradiate the active Streptomyces monospore liquid with an ultraviolet lamp at a distance of 35 cm from the single spore liquid for 20 to 30 seconds, and after dilution and cultivation for 6 to 7 days, select a single colony with better growth and inoculate it on a slant medium to obtain Mutated active Streptomyces CN10.

[0043] Step 2, the active Streptomyces CN10 of the mutation that step 1 obtains is treated with the Tris-Hcl solution of pH6.0, obtains the second spore suspension, and nitrosoguanidine is dissolved in the Tris-Hcl solution of pH6.0, obtains nitrosoguanidine The stock solution of nitrosoguanidine, and then mix the second spore suspension and the stock solution of nitrosoguanidine at a volume ratio of 1:1, so that the concentration of nitrosoguanidine is 300-500 μg / mL, and then at 26-30°C Mutagenesis was performed for 120-130 minutes. After the ...

Embodiment 3

[0046] Such as figure 1 Shown, a kind of method utilizing described active streptomyces actuosus LB-16 to prepare nosiheptide, comprises the steps:

[0047] A. Pick fresh high-yield active Streptomyces LB-16 from the parent plant test tube, insert it into 80mL seed medium, and put the seed medium into a 500mL Erlenmeyer flask at a temperature of 22°C and a speed of 120rpm , shaking culture for 72h, as a primary seed.

[0048] B. Insert 50mL of the above-mentioned primary seeds on 200mL of seed medium, and put the seed medium into a 1000mL Erlenmeyer flask, and vibrate for 60 hours at a temperature of 22°C and a rotation speed of 120rpm, and then use it as a secondary seed.

[0049] C. Put the above-mentioned secondary seeds into a 100L fully automatic fermenter equipped with fermentation medium, with a liquid volume of 60L, a temperature of 22°C, a rotation speed of 150rpm, an air volume ratio of 1:0.4, and a pressure of 0.6 Under KPa, ferment and cultivate for 120h. When f...

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Abstract

The invention relates to Streptomyces actuosus LB-16, and further relates to a method for producing Streptomyces actuosus LB-16 and a method for preparing nosiheptide from the Streptomyces actuosus LB-16. In the method for producing the Streptomyces actuosus LB-16, ultraviolet and nitrosoguanidine are adopted for achieving combined mutagenesis for Streptomyces actuosus CN10, and the Streptomyces actuosus LB-16 is obtained through screening. In the method for preparing the nosiheptide from the Streptomyces actuosus LB-16, temperature-variable culture is adopted, the ventilation flow is controlled in a staging mode, and an organic solvent method, resin separation, and ethyl alcohol and water mixed precipitation extraction separation are adopted, so that the nosiheptide is obtained, and the refined nosiheptide is obtained by the adoption of an ion exchange chromatography through separation and purification. The Streptomyces actuosus LB-16 has the advantages of being low in production cost, high in production extraction rate and high in product biological value.

Description

technical field [0001] The present invention relates to an active streptomyces. More specifically, the present invention relates to a Streptomyces mobilis LB-16 and a method for preparing nosiheptide using it. Background technique [0002] Nosiheptide, also known as Nosiheptide, is a polypeptide antibiotic containing multiple thiazole rings and rich in sulfur. It is combined with Thiostreton, siomyein and Mierococcin ), Thiopeptin (Tiopeptin) belong to the same class of compounds. As a new type of feed additive, nosiheptide has been widely recognized at home and abroad. It can not only promote animal growth and improve feed efficiency, but also has the characteristics of not easy to produce drug resistance, no residue, high safety and environmental friendliness And other characteristics, the product is also widely used in Japan, Southeast Asia and China Taiwan and other places. But at present, nosiheptide needed by domestic enterprises mainly depends on imports. [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C12P21/02C12R1/465
CPCC12N15/01C12P21/02C12N1/205C12R2001/465
Inventor 张春颖
Owner 西藏天虹科技股份有限责任公司
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