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Filling and acquiring devices for extracting hematopoietic stem cells from placenta

A hematopoietic stem cell and collection device technology, applied in the field of perfusion collection devices, can solve the problems of high pollution rate, low perfusion efficiency, and damage to placental integrity

Active Publication Date: 2016-06-22
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the perfusion efficiency of the existing perfusion method is low, and the placenta is interspersed many times, which destroys the integrity of the placenta and causes problems such as high pollution rate.

Method used

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  • Filling and acquiring devices for extracting hematopoietic stem cells from placenta
  • Filling and acquiring devices for extracting hematopoietic stem cells from placenta

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0056] The present invention also provides a method for preparing placental hematopoietic stem cells, including the following steps:

[0057] a) The first perfusion needle and the second perfusion needle in the perfusion device according to any one of the above technical solutions are respectively introduced into the two umbilical arteries of the placenta, and the collection in the collection device according to any one of the above technical solutions The needle is introduced into the umbilical vein of the placenta;

[0058] b) After the perfusion treatment is performed by the perfusion device according to any one of the above technical solutions, the collection fluid is collected by the collection device according to any one of the above technical solutions, and directly subjected to post-processing to obtain placental hematopoietic stem cells.

[0059] The preferred solutions on the above-mentioned device of the present invention are the same as those described in the previous dev...

Embodiment 1

[0066] First, a perfusion device for extracting hematopoietic stem cells from the placenta is provided, which includes a perfusion inlet, a tee, a first perfusion needle and a second perfusion needle. One end of the perfusion inlet is connected to the inlet end of the Y-shaped tee through a catheter with an inner diameter of 5mm, and the first outlet end of the Y-shaped tee is connected to the first perfusion needle through a first catheter with an inner diameter of 5mm. The second outlet end of the Y-shaped tee is connected to a second intravenous infusion needle (No. 7) through a second catheter with an inner diameter of 5 mm. The first perfusion needle is an intravenous infusion needle (No. 7), the first perfusion needle is provided with a needle handle and a fixing thread, the second perfusion needle is an intravenous infusion needle (No. 7), and the second perfusion needle is provided with a needle handle And fixed with thin wires. See figure 1 .

[0067] Secondly, a colle...

Embodiment 2

[0069] The perfusion device and collection device provided in Example 1 of the present invention are used in the process of extracting hematopoietic stem cells from the placenta, that is, in the process of preparing placental hematopoietic stem cells, the first perfusion needle and the second perfusion needle of the perfusion device are respectively introduced into the placenta In the two umbilical arteries, the collection needle in the collection device is introduced into the umbilical vein of the placenta;

[0070] Use a 50 mL disposable syringe to push the perfusate into the two arteries in the placenta until the serum in the placenta is cleaned. Use a 50mL disposable syringe to suck the enzymatic hydrolysate into the two arteries in the placenta. When the fluid flowing out of the vein is enzymatic hydrolysate, ligate the arteries and veins and place them at room temperature for enzymolysis. Loosen the vein, a 50mL disposable syringe sucks the lavage fluid into the two arterie...

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Abstract

The invention provides a filling device and an acquiring device for extracting hematopoietic stem cells (HSC) from a placenta. The filling device comprises a filling inlet, a T-joint, a first filling needle and a second filling needle, wherein one end of the filling inlet is connected with the inlet end of the T-joint, the first outlet end of the T-joint is connected with the first filling needle, and the second outlet end of the T-joint is connected with the second filling needle. The acquiring device comprises a catheter and an acquisition needle, wherein one end of the catheter is connected with the acquisition needle, and the acquisition needle is provided with a fixing device. By the adoption of double syringe needles, the filling needles can be fixed for placenta filling, contact between HSC and the external environment is avoided all through the process of separating and extracting HSC from the placenta, and the contamination rate is reduced greatly; furthermore, continuous filling can be achieved, and filling efficiency is improved greatly.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a perfusion collection device for extracting hematopoietic stem cells from the placenta. Background technique [0002] Hemopoietic stem cells (Hemopoietic Stemcell, HSC) are a group of primitive hematopoietic cells that exist in hematopoietic tissues. They are not fixed tissue cells, but can exist in hematopoietic tissues and blood. Hematopoietic stem cells are the origin of all hematopoietic cells and immune cells, and have the ability to self-renew and multi-directional differentiation. Its basic characteristic is self-renewal ability, that is, after a cell cycle activity, it can produce two hematopoietic stem cells with the same properties as before division, and at the same time have the ability of multidirectional differentiation, that is, under certain environmental conditions, hematopoietic stem cells have The ability to differentiate into various blood cells. [0003] With the in-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/0789
CPCC12M29/10C12M47/04C12N5/0647C12N2509/00
Inventor 葛啸虎陈海佳王一飞黎娟妹
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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