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Tissue culture fast propagation method for garcinia paucinervis

A technology of tissue culture rapid propagation and golden plum, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high requirements for seed preservation, high processing requirements, and long breeding time, and achieve the goal of promoting the flow of auxin Plant system, good root tropism, and the effect of promoting the growth of tap roots

Inactive Publication Date: 2016-06-22
覃伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It can be seen that Golden Plum reproduces offspring by directly sowing seeds. The disadvantages are high requirements for seed preservation and treatment, long breeding time and low emergence rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for tissue culture and rapid propagation of golden silk plum, comprising the following steps:

[0026](1) Disinfection of explants: Pick plum leaves with bright green color, completeness, and no pests and diseases at 1:00 p.m. in June, use 20% dimethoate emulsion to dilute 400 times for soaking for 5 minutes, take them out and put them on an ultra-clean workbench Use 4% vitamin C aqueous solution to soak for 30 minutes, then disinfect with 3% hypochlorous acid for 5 minutes, then rinse with sterile water for 3 times, and finally use sterile filter paper to absorb the sterile water on the surface of Prunus chinensis leaves;

[0027] (2) Callus culture: use a scalpel to cut out leaf strips with a length of 15 cm and a width of 10 cm along the stem position of the Prunus chinensis leaf, and put 3 leaf strips into the petri dish that has been placed in 1 / In a petri dish of 3 volumes of callus medium, lightly press the leaf strips, cultivate in the dark for 8 days...

Embodiment 2

[0031] A method for tissue culture and rapid propagation of golden silk plum, comprising the following steps:

[0032] (1) Disinfection of explants: Pick plum leaves with bright green color, completeness, and no pests and diseases at 2 o'clock in the evening in July, use 40% dimethoate emulsion to dilute 600 times of liquid to soak for 10 minutes, take them out and put them on an ultra-clean workbench Use 4% vitamin C aqueous solution to soak for 30 minutes, then disinfect with 3% hypochlorous acid for 10 minutes, then rinse with sterile water for 5 times, and finally use sterile filter paper to absorb the sterile water on the surface of the leaves of Prunus chinensis;

[0033] (2) Callus culture: use a scalpel to cut out leaf strips with a length of 18 cm and a width of 15 cm along the stem position of the Prunus chinensis leaf, and put 5 leaf strips into the petri dish that has been placed in 1 / In a petri dish with 2 volumes of callus medium, lightly press the leaf strips,...

Embodiment 3

[0037] A method for tissue culture and rapid propagation of golden silk plum, comprising the following steps:

[0038] (1) Disinfection of explants: Pick plum leaves with bright green color, completeness, and no pests and diseases at 1:00 pm in June, use 30% dimethoate emulsion to dilute 500 times for immersion for 8 minutes, take them out and put them on an ultra-clean workbench Use 4% vitamin C aqueous solution to soak for 30 minutes, then disinfect with 3% hypochlorous acid for 8 minutes, then rinse with sterile water for 4 times, and finally use sterile filter paper to dry the sterile water on the surface of Prunus chinensis leaves;

[0039] (2) Callus culture: use a scalpel to cut out leaf strips with a length of 17 cm and a width of 13 cm along the stem position of the Prunus chinensis leaf, and put 4 leaf strips into the petri dish that has been placed in 1 / In a petri dish of 3 volumes of callus medium, lightly press the leaf strips, cultivate at 30°C for 10 days, and...

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PUM

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Abstract

The invention belongs to the technical field of tissue culture production and relates to a tissue culture fast propagation method for garcinia paucinervis.The method includes the following steps of 1, disinfection of an explant; 2, callus culture; 3, callus bud differentiation; 4, bud rooting.The formula of a root inducing medium is MS + 6-8g / L agar + 25-29g / L saccharose + 0.8-2.5mg / L indoleacetic acid + 0.5-1.5mg / L kinetin + 0.8-1.5mg / L activated carbon + 0.1-0.5mg / L metaxamin + 0.5-1.5mg / L compound sodium nitrophenolate + 0.3-0.8mg / L vitamin D2 + 0.15-0.35mg / L microcystin.The tissue culture fast propagation method has the advantages that propagation cost can be reduced, the growth period is short, the propagation rate is high, management is convenient, industrial production and automatic monitoring are benefited, and manpower, material resources and land for field crops are greatly saved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture production, and relates to a tissue culture and rapid propagation method of golden silk plum. Background technique [0002] Plum chinensis is an evergreen tree with a height of 30 meters and a DBH of more than 1.5 meters; the bark is grayish brown. Cannulate opposite, leathery, oblong, 6-18cm long, 2-6cm wide, entire, apex acuminate or short acuminate, blunt, base broadly cuneate, lateral veins 5-8 pairs, below Conspicuously raised; petiole 6-15 mm long. Flowers polygamy on the same plant; male flowers in axillary and terminal cymes, sepals 4, suborbicular, petals 4, ovate, stamens numerous, filaments short, connate into 4-lobed rings, anthers 2-loculed, longitudinally fissured, Pistil nearly quadrangular, stigma peltate protruding; female flower usually solitary in leaf axils, larger than male flower, stigma peltate, middle raised, ovary spherical, about 2.5 mm in diameter, with 1 basal ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 覃伟
Owner 覃伟
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