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An optical rotation detection device for capillary electrophoresis

A technology of capillary electrophoresis and optical detection device, which is applied in measurement devices, material analysis by electromagnetic means, material analysis by optical means, etc. Effect of lack of enantiomeric standard

Inactive Publication Date: 2018-09-07
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the detection amount of the two differs too much and it is difficult to match
Moreover, due to the limitation of the detection optical path and mechanical processing in the optical rotation detection technology, it is difficult to further reduce the volume of the sample chamber for the optical rotation detection
At present, there is no report on the detection method of connecting capillary electrophoresis with an optical rotation detector

Method used

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  • An optical rotation detection device for capillary electrophoresis
  • An optical rotation detection device for capillary electrophoresis
  • An optical rotation detection device for capillary electrophoresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A self-made polarimeter is used, the minimum detection angle of the polarimeter is 0.002 degrees, and the integration time is set to 10 seconds. The on-line detection cell is fixed in the detection chamber of the polarimeter, and the outlet end of the capillary separation column 5 of capillary electrophoresis is inserted into the inlet of the PEEK tube 10 by 1 cm through a tee. The mandelic acid sample solution was injected by differential pressure, and the separation was performed by chiral capillary electrophoresis. figure 2 It is the chiral capillary electrophoresis spectrum of mandelic acid detected by optical rotation.

Embodiment 2

[0025] A self-made polarimeter is used, the minimum detection angle of the polarimeter is 0.002 degrees, and the integration time is set to 10 seconds. The on-line detection cell is fixed in the detection chamber of the polarimeter, and the outlet end of the capillary separation column 5 of capillary electrophoresis is inserted into the inlet of the PEEK tube 10 by 1 cm through a tee. The phenylglycine sample solution was injected by differential pressure, and resolved by chiral capillary electrophoresis. image 3 It is the chiral capillary electrophoresis structure map of phenylglycine detected by optical rotation.

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Abstract

The invention discloses an optical rotation detection device used for capillary electrophoresis, and belongs to the technical field of photoelectric detection. The basic scheme of the optical rotation detection device is disclosed in the abstract drawing. The invention aims to provide a chirality optical rotation detection method for the capillary electrophoresis, optical rotation detection can be provided for detected liquid output by the capillary electrophoresis, and the chirality characteristics of the detected liquid are analyzed. According to the characteristics of a capillary electrophoresis apparatus and of an optical rotation detector, the invention designs a special connection device, and auxiliary equipment and liquid flow are adopted to realize the compatible connection of small-volume capillary electrophoresis output liquid and large-volume optical rotation liquid to be detected. In addition, corresponding improvement is made for a subsequent optical rotation detector to improve the detection accuracy of the optical rotation detection device. The scheme adopted by the invention is the first on-line optical rotation detection scheme of the capillary electrophoresis in the world.

Description

technical field [0001] The invention is a new technical solution for optical rotation detection of on-line capillary electrophoresis, provides a basis for the optical rotation detection of capillary electrophoresis, and belongs to the fields of photoelectric detection technology and chromatographic optical rotation detection. Background technique [0002] Chiral compounds have the same molecular weight, but have completely different properties due to their different structural symmetries, so it is very important to separate and characterize their chiral properties. Since the UV-Vis and fluorescence detectors commonly used in separation techniques cannot distinguish the left-right characteristics of chiral substances, it is necessary to supplement with optical rotation detection technology to determine the left-handed or right-handed properties of compounds. Therefore, Polarization Detection technology has become a key technology to characterize the symmetry of chiral compoun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/21G01N27/447
CPCG01N21/211G01N27/447
Inventor 袁黎明李宏宁朱鹏静章俊辉陶勇
Owner YUNNAN NORMAL UNIV
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