Method for efficiently and rapidly inhibiting endogenous-gene expression of cynodon dactylon
A bermudagrass and fast technology, applied in the fields of genetic engineering and biology, can solve the problem of lack of bermudagrass virus-induced gene silencing technology, etc., and achieve the effect of improving the infection efficiency
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[0021] Example 1 The gene silencing of Bermudagrass phytoene dehydrogenase leads to albinism of plant leaves.
[0022] State-approved bermudagrass variety 'Yangjiang' bermudagrass and 'common' bermudagrass imported from the United States: Grass Industry Research Center, Institute of Botany, Chinese Academy of Sciences, Jiangsu Province. pRTBV-MVIGS plasmid: Department of Plant Molecular Biology, South Campus, University of Delhi, India (References: Purkayastha A, Mathur S, Verma V, Sharma S, Dasgupta I. Virus-induced gene silencing in rice using avector derived from a DNA virus. Planta, 2010, 232: 1531-1540). Escherichia coli DH5α and Agrobacterium EHA105: Grassland Research Center, Institute of Botany, Chinese Academy of Sciences, Jiangsu Province.
[0023] 1. Cloning of Bermudagrass phytoene dehydrogenase gene (phytoenedesaturase, PDS).
[0024] The total RNA of 'Yangjiang' bermudagrass leaves was extracted using the RNAprep plant total RNA extraction kit from Tiangen Bioch...
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