New lactic acid bacterium, natural immunostimulant having new lactic acid bacterium as active ingredient, and food or drink containing new lactic acid bacterium

A technology of natural immunity and lactic acid bacteria, which is applied in the field of food and beverages containing new lactic acid bacteria, can solve the problems of undiscovered CRISPR-Cas9 and achieve high resistance effect

Active Publication Date: 2016-06-01
GENOME PHARMA INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is believed that double-stranded RNA is formed in the CRISPR-Cas9 pathway, but CRISPR-Cas9 has not been found in the genome sequence of Lactococcus lactis

Method used

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  • New lactic acid bacterium, natural immunostimulant having new lactic acid bacterium as active ingredient, and food or drink containing new lactic acid bacterium
  • New lactic acid bacterium, natural immunostimulant having new lactic acid bacterium as active ingredient, and food or drink containing new lactic acid bacterium
  • New lactic acid bacterium, natural immunostimulant having new lactic acid bacterium as active ingredient, and food or drink containing new lactic acid bacterium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0201]

[0202] After the 11 / 19-B1 strain cultured overnight in GAM medium was sterilized at 121°C for 20 minutes, 50 μL was injected into decapitated muscle specimens of 5th instar silkworms. ) to measure innate immune activation activity.

[0203] The innate immune activation activity based on slow muscle contraction was measured according to the method described in Ishii K., Hamamoto H., Kamimura M., Sekimizu K., J. Biol. Chem. Jan. 25; 283(4): 2185-91 (2008) to proceed.

[0204] That is, 0.05 mL of the above-mentioned sample was administered into the blood of a decapitated muscle specimen of a fifth-instar silkworm, and the body length was measured when the C value was maximum (about 10 minutes later), and the body length after injection was subtracted from the body length before injection. The length of the body is divided by the body length before injection, and the obtained value is the measured C value (contraction value, ContractionValue).

Embodiment 2

[0224]

[0225] >

[0226] The cultured 11 / 19-B1 strain was sterilized at 121° C. for 20 minutes, and then concentrated. 20.0 mg of the concentrated 11 / 19-B1 strain culture solution, 40 mg of lactose, 20 mg of starch, and 5 mg of low-substituted hydroxypropylcellulose were uniformly mixed, and an 8% by mass aqueous solution of hydroxypropylmethylcellulose was used as a binder Agent, using wet granulation method to manufacture granules for tableting. 0.5 mg to 1 mg of magnesium stearate necessary for providing lubricity is added thereto, and the tablet is compressed using a tablet machine to form tablets.

[0227] >

[0228] 10.0 mg of the concentrated 11 / 19-B1 strain culture solution was dissolved in 10 mL of a 2 mass % 2-hydroxypropyl-β-cyclodextrin aqueous solution to prepare a liquid preparation for injection.

Embodiment 3

[0230]

[0231] Milk was sterilized at 95° C. for 5 minutes, then cooled to 40° C., and 0.001 parts by mass of 11 / 19-B1 strain was added. Then, it fermented at 37 degreeC for 72 hours, and obtained fermented milk. After cooling this fermented milk at 10° C. or lower, flavor and physical properties were confirmed.

[0232] As a result, both flavor and physical properties were extremely favorable.

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PUM

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Abstract

The present invention pertains to a lactic acid bacterium belonging to the genus Lactococcus and having a receipt number NITE ABP-01694 with the Patent Microorganisms Depositary (NPMD) of the National Institute of Technology and Evaluation (NITE), a natural immunostimulant having this lactic acid bacterium as an active ingredient, a food or drink containing this lactic acid bacterium or this natural immunostimulant, and yogurt containing lactic acid bacteria, wherein the yogurt has resistance to at least one bacterium selected from the group comprising methicillin-sensitive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus mundtii.

Description

【Technical field】 [0001] The present invention relates to a novel lactic acid bacterium, a natural immune activator containing the novel lactic acid bacterium as an active ingredient, and a food and drink containing the novel lactic acid bacterium. 【Background technique】 [0002] Lactic acid bacteria have been used to ferment food since ancient times, and are used industrially in the production of food, pharmaceuticals, and probiotics. Lactic acid bacteria are Gram-positive, catalase-negative, non-endospore-forming, and non-motile. [0003] It is known that Lactococcus lactis (L. lactis) is a non-pathogenic Gram-positive lactic acid bacterium, which does not belong to the human intestinal flora, and does not form colonies in the oral cavity and intestinal tract. In addition, its whole genome has been deciphered, and analyzes of genetics, vector system, and gene expression system have also been carried out. Recently, it has been reported that recombinant Lactococcus lactis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12A23L33/135A61K35/744A61P37/04C12R1/01
CPCA23C9/123C12N1/20A23V2002/00A61K35/744A23L33/135A61P37/04C12R2001/46C12N1/205A23V2400/231A23V2200/324A61K35/74
Inventor 关水和久西田智
Owner GENOME PHARMA INST
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