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Method for production of refined dried ramie by microbial blending and degumming

A technology for ramie essence, dried hemp and microorganisms, applied in the field of microorganisms, can solve the problems of inability to completely decompose the compatibility of various components of ramie colloid, and achieve the effects of reducing comprehensive production costs, rapid removal, and low breakage rate.

Active Publication Date: 2016-06-01
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a method for producing ramie fine-dried hemp by mixing and degumming microorganisms, and solve the problems in the prior art that one bacterial strain cannot completely decompose various components of ramie colloid, and two kinds of microorganisms are mixed and fermented and cultivated. Capacitance and other issues

Method used

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  • Method for production of refined dried ramie by microbial blending and degumming

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Experimental program
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Effect test

Embodiment 1

[0041] (1) Preservation, activation and expanded cultivation of Bacillus sp.HG-116

[0042] Strain preservation: Inoculate Bacillus sp.HG-116 strains into 250mL shake flasks filled with 100mL No. 1 medium according to the inoculum amount of 0.5%, and cultivate them at 30°C and 120r / min for 6 hours. The final bacterial solution was stored at -20°C with the glycerol seed preservation method to obtain the HG-116 strain tube;

[0043] Activation: Place the HG-116 strain tube at room temperature for 3 minutes until the bacterial solution melts, inoculate 2.5% of the inoculum into a 1L shaker flask filled with 250mL No. Cultivate under the condition for 1.5h to obtain the activated strain of HG-116.

[0044] Expanded cultivation: The activated strain HG-116 was inoculated into a 50L primary seed tank with 30L No. 3 Cultivate for 7 hours under the condition of air flow per hour. Then inoculate the bacterium solution of the first-level seed tank into the second-level seed tank of 1...

Embodiment 2

[0063] (1) Preservation, activation and expanded cultivation of Bacillus sp.HG-116

[0064] Strain storage: Inoculate Bacillus sp.HG-116 strains into 250mL shake flasks filled with 60mL No. 1 medium according to the inoculum amount of 2.5%, and cultivate them at 40°C and 200r / min for 1.5h. The cultured bacterial solution was stored at -20°C with the glycerol seed preservation method to obtain the HG-116 strain tube;

[0065] Activation: Place the HG-116 strain tube at room temperature for 3 minutes until the bacterial solution melts, inoculate 0.5% of the inoculum into a 1L shaker flask filled with 250mL No. Cultivate under the condition for 6h to obtain the activated strain of HG-116.

[0066] Expanded cultivation: the activated strain HG-116 was inoculated into a 50L primary seed tank with 30L No. 3 / h ventilation conditions for 2h. Then inoculate the bacterium solution of the first-level seed tank into the second-level seed tank of 1t with the specification of 600L No. t...

Embodiment 3

[0085] (1) Preservation, activation and expanded cultivation of Bacillus sp.HG-116

[0086] Strain preservation: Inoculate Bacillus sp.HG-116 strains into 250mL shake flasks filled with 80mL No. 1 medium according to the inoculum amount of 1%, and cultivate them at 36°C and 180r / min for 3 hours. The final bacterial solution was stored at -20°C with the glycerol seed preservation method to obtain the HG-116 strain tube;

[0087] Activation: Place the HG-116 strain tube at room temperature for 3 minutes until the bacterial solution melts, then inoculate 1.2% of the inoculum into a 1L shaker flask containing 250mL No. Cultivate under the condition for 4h to obtain the activated strain of HG-116.

[0088] Expanded cultivation: the activated strain HG-116 was inoculated into a 50L primary seed tank with 30L No. 3 / h ventilation conditions for 4h. Then inoculate the bacterium solution of the first-level seed tank into the second-level seed tank of 1t with the specification of 600...

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Abstract

The invention relates to a method for production of refined dried ramie by microbial blending and degumming, Bacillus Bacillus sp., HG-116 and Bacillus Bacillus sp., HG-247 are used as strains for respective strain activation step and enlarged cultivation step to obtain a bacterium fluid, and the bacterium fluid and water are mixed for fermentation cultivation, enzyme production cultivation and biological degumming to achieve the purpose of rapid and thorough removal of various ramie gum components. After optimization of types of culture mediums and fermentation conditions, two kinds of microorganisms can grow well, cultivation period is short, technical indexes of the produced refined dried ramie are far above the national standards of first-level refined dried ramie, the refined dried ramie is very fluffy due to super low residual gum rate, a spinning pretreatment process is simple, dust is significantly reduced, spinning end breakage rate is low, ramie yarn comprehensive production cost is reduced, and ramie yarn quality is improved significantly.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a method for producing ramie fine-dried hemp by mixing and degumming microorganisms. Background technique [0002] In addition to cellulose, ramie bast also contains colloidal components such as hemicellulose, pectin, and lignin. These colloids wrap the fibers and make them cemented together to form a hard sheet-like bundle. It is necessary to remove the colloids in a certain way to obtain fiber raw materials for spinning and weaving. The degumming method is directly related to the quality of ramie fiber, as well as the environmental protection problems caused by degumming wastewater and waste residue. [0003] Microbial degumming is to inoculate ramie bast with strains capable of degrading gelatin, and the degumming bacteria secrete degumming enzymes to degrade ramie gelatin, and use the degradation products as nutrient sources for growth and reproduction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01C1/00C12N1/20C12R1/07
Inventor 余龙江樊培李为周蓬蓬张非杨玉珍胡祖德胡振华
Owner HUAZHONG UNIV OF SCI & TECH
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